PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains

Detalhes bibliográficos
Autor(a) principal: Clementino, Maysa Beatriz Mandetta
Data de Publicação: 2001
Outros Autores: Filippis, Ivano de, Nascimento, Carlos Roberto Sobrinho do, Branquinho, Maria Regina, Rocha, Carmem Lucia, Martins, Orlando Bonifácio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11284
http://dx.doi.org/10.1128/jcm.39.11.3865-3870.2001
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.
id CRUZ_d5b59f048fe35534ac3d99cff23af297
oai_identifier_str oai:www.arca.fiocruz.br:icict/11284
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Clementino, Maysa Beatriz MandettaFilippis, Ivano deNascimento, Carlos Roberto Sobrinho doBranquinho, Maria ReginaRocha, Carmem LuciaMartins, Orlando Bonifácio2015-07-13T17:56:02Z2015-07-13T17:56:02Z2001CLEMENTINO, M. B. M. et al. PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains. J. Clin. Microbiol., Washington DC, v. 39, n. 11, p. 3865-3870, 2001.0095-1137https://www.arca.fiocruz.br/handle/icict/11284http://dx.doi.org/10.1128/jcm.39.11.3865-3870.2001engAmerican Society for MicrobiologyDNA IntergênicoDNA Espaçador RibossômicoEnterobacter cloacaeInfecções por EnterobacteriaceaeFilogeniaReação em Cadeia da PolimeraseTécnica de Amplificação ao Acaso de DNA PolimórficoDNA, IntergenicDNA, Ribosomal SpacerEnterobacter cloacaeEnterobacteriaceae InfectionsPhylogenyPolymerase Chain ReactionRandom Amplified Polymorphic DNA TechniqueDNA IntergênicoDNA Espaçador RibossômicoEnterobacter cloacaeInfecções por EnterobacteriaceaeFilogeniaReação em Cadeia da PolimeraseTécnica de Amplificação ao Acaso de DNA PolimórficoPCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strainsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Centro de Ciência da Saúde. Rio de Janeiro, RJ, Brasil.PCR analysis of tRNA intergenic spacer (tDNA-PCR) and of the 16S-23S internal transcribed spacer (ITS-PCR) and random amplified polymorphic DNA (RAPD) analysis were evaluated for their usefulness in characterization of Enterobacter cloacae strains isolated from both clinical origins and vaccine microbial contamination. tDNA-PCR presented specific and reproducible patterns for Enterobacter sakazakii ATCC 29004, Enterobacter aerogenes ATCC 13048, and Enterobacter cloacae ATCC 13047 and 23355 that presented the same profile for all 16 E. cloacae isolates, offering an alternative tool for species-level identification. ITS-PCR and RAPD analysis yielded completely different banding patterns for the 20 strains studied, except for E. cloacae strains isolated from different batches of vaccine that exhibited a unique pattern, suggesting contamination by the same strain. The combined use of tDNA-PCR and ITS-PCR in a one-step protocol allows accurate identification and typing of E. cloacae strains a few hours after the colony has been isolated.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/11284/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALJ_Clin_Microbiol_39_3865-3870.pdfJ_Clin_Microbiol_39_3865-3870.pdfapplication/pdf1005017https://www.arca.fiocruz.br/bitstream/icict/11284/2/J_Clin_Microbiol_39_3865-3870.pdf9856b55849af30df4fd8367911252d9dMD52TEXTJ_Clin_Microbiol_39_3865-3870.pdf.txtJ_Clin_Microbiol_39_3865-3870.pdf.txtExtracted texttext/plain30039https://www.arca.fiocruz.br/bitstream/icict/11284/3/J_Clin_Microbiol_39_3865-3870.pdf.txt846eaec79d8eb049756742f9ad364aa2MD53icict/112842018-04-04 07:58:16.196oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-04T10:58:16Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en.fl_str_mv PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains
title PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains
spellingShingle PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains
Clementino, Maysa Beatriz Mandetta
DNA Intergênico
DNA Espaçador Ribossômico
Enterobacter cloacae
Infecções por Enterobacteriaceae
Filogenia
Reação em Cadeia da Polimerase
Técnica de Amplificação ao Acaso de DNA Polimórfico
DNA, Intergenic
DNA, Ribosomal Spacer
Enterobacter cloacae
Enterobacteriaceae Infections
Phylogeny
Polymerase Chain Reaction
Random Amplified Polymorphic DNA Technique
DNA Intergênico
DNA Espaçador Ribossômico
Enterobacter cloacae
Infecções por Enterobacteriaceae
Filogenia
Reação em Cadeia da Polimerase
Técnica de Amplificação ao Acaso de DNA Polimórfico
title_short PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains
title_full PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains
title_fullStr PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains
title_full_unstemmed PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains
title_sort PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains
author Clementino, Maysa Beatriz Mandetta
author_facet Clementino, Maysa Beatriz Mandetta
Filippis, Ivano de
Nascimento, Carlos Roberto Sobrinho do
Branquinho, Maria Regina
Rocha, Carmem Lucia
Martins, Orlando Bonifácio
author_role author
author2 Filippis, Ivano de
Nascimento, Carlos Roberto Sobrinho do
Branquinho, Maria Regina
Rocha, Carmem Lucia
Martins, Orlando Bonifácio
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Clementino, Maysa Beatriz Mandetta
Filippis, Ivano de
Nascimento, Carlos Roberto Sobrinho do
Branquinho, Maria Regina
Rocha, Carmem Lucia
Martins, Orlando Bonifácio
dc.subject.other.pt_BR.fl_str_mv DNA Intergênico
DNA Espaçador Ribossômico
Enterobacter cloacae
Infecções por Enterobacteriaceae
Filogenia
Reação em Cadeia da Polimerase
Técnica de Amplificação ao Acaso de DNA Polimórfico
topic DNA Intergênico
DNA Espaçador Ribossômico
Enterobacter cloacae
Infecções por Enterobacteriaceae
Filogenia
Reação em Cadeia da Polimerase
Técnica de Amplificação ao Acaso de DNA Polimórfico
DNA, Intergenic
DNA, Ribosomal Spacer
Enterobacter cloacae
Enterobacteriaceae Infections
Phylogeny
Polymerase Chain Reaction
Random Amplified Polymorphic DNA Technique
DNA Intergênico
DNA Espaçador Ribossômico
Enterobacter cloacae
Infecções por Enterobacteriaceae
Filogenia
Reação em Cadeia da Polimerase
Técnica de Amplificação ao Acaso de DNA Polimórfico
dc.subject.en.en.fl_str_mv DNA, Intergenic
DNA, Ribosomal Spacer
Enterobacter cloacae
Enterobacteriaceae Infections
Phylogeny
Polymerase Chain Reaction
Random Amplified Polymorphic DNA Technique
dc.subject.decs.pt_BR.fl_str_mv DNA Intergênico
DNA Espaçador Ribossômico
Enterobacter cloacae
Infecções por Enterobacteriaceae
Filogenia
Reação em Cadeia da Polimerase
Técnica de Amplificação ao Acaso de DNA Polimórfico
description Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2015-07-13T17:56:02Z
dc.date.available.fl_str_mv 2015-07-13T17:56:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CLEMENTINO, M. B. M. et al. PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains. J. Clin. Microbiol., Washington DC, v. 39, n. 11, p. 3865-3870, 2001.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11284
dc.identifier.issn.none.fl_str_mv 0095-1137
dc.identifier.doi.none.fl_str_mv http://dx.doi.org/10.1128/jcm.39.11.3865-3870.2001
identifier_str_mv CLEMENTINO, M. B. M. et al. PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains. J. Clin. Microbiol., Washington DC, v. 39, n. 11, p. 3865-3870, 2001.
0095-1137
url https://www.arca.fiocruz.br/handle/icict/11284
http://dx.doi.org/10.1128/jcm.39.11.3865-3870.2001
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/11284/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/11284/2/J_Clin_Microbiol_39_3865-3870.pdf
https://www.arca.fiocruz.br/bitstream/icict/11284/3/J_Clin_Microbiol_39_3865-3870.pdf.txt
bitstream.checksum.fl_str_mv 8b4c200b4e10021c5683c6ccaba07169
9856b55849af30df4fd8367911252d9d
846eaec79d8eb049756742f9ad364aa2
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324885203714048

Registros relacionados