Multiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Responders

Detalhes bibliográficos
Autor(a) principal: Alves, Jéssica Rafaela dos Santos
Data de Publicação: 2021
Outros Autores: Araújo, Fernanda Fortes de, Pires, Camilla V., Carvalho, Andréa Teixeira de, Lima, Barbara Andreza Soares, Torres, Letícia de Menezes, Ntumngia, Francis B., Adams, John H., Kano, Flora Satiko, Carvalho, Luzia Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/53266
Resumo: Molecular Biology and Malaria Immunology. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
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spelling Alves, Jéssica Rafaela dos SantosAraújo, Fernanda Fortes dePires, Camilla V.Carvalho, Andréa Teixeira deLima, Barbara Andreza SoaresTorres, Letícia de MenezesNtumngia, Francis B.Adams, John H.Kano, Flora SatikoCarvalho, Luzia Helena2022-06-14T17:47:46Z2022-06-14T17:47:46Z2021ALVES, Jéssica Rafaela dos Santos et al. Multiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Responders. Front Immunol., v. 12, 704653, 2021. doi: 10.3389/fimmu.2021.704653.1664-3224https://www.arca.fiocruz.br/handle/icict/5326610.3389/fimmu.2021.704653engFrontiers Research FoundationMultiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Respondersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMolecular Biology and Malaria Immunology. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Integrated Research Group in Biomarkers. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Center for Global Health and Infectious Diseases Research. Department of Global Health. College of Public Health. University of South Florida. Tampa, FL, United States.Integrated Research Group in Biomarkers. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Molecular Biology and Malaria Immunology. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Molecular Biology and Malaria Immunology. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Center for Global Health and Infectious Diseases Research. Department of Global Health. College of Public Health. University of South Florida. Tampa, FL, United States.Center for Global Health and Infectious Diseases Research. Department of Global Health. College of Public Health. University of South Florida. Tampa, FL, United States.Molecular Biology and Malaria Immunology. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Molecular Biology and Malaria Immunology. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Malaria remains a major public health problem worldwide, and Plasmodium vivax is the most widely distributed malaria parasite. Naturally acquired binding inhibitory antibodies (BIAbs) to region II of the Duffy binding protein (DBPII), a P. vivax ligand that is critical for reticulocyte invasion, are associated with a reduced risk of clinical malaria. Owing to methodological issues in evaluating antibodies that inhibit the DBPII-DARC interaction, a limited number of studies have investigated DBPII BIAbs in P. vivax-exposed populations. Based on the assumption that individuals with a consistent BIAb response are characterized by strain-transcending immune responses, we hypothesized that detecting broadly reactive DBPII antibodies would indicate the presence of BIAb response. By taking advantage of an engineered DBPII immunogen targeting conserved DBPII neutralizing epitopes (DEKnull-2), we standardized a multiplex flow cytometry-based serological assay to detect broadly neutralizing IgG antibodies. For this study, a standard in vitro cytoadherence assay with COS-7 cells expressing DBPII was used to test for DBPII BIAb response in long-term P. vivax-exposed Amazonian individuals. Taken together, the results demonstrate that this DBPII-based multiplex assay facilitates identifying DBPII BIAb carriers. Of relevance, the ability of the multiplex assay to identify BIAb responders was highly accurate when the positivity for all antigens was considered. In conclusion, the standardized DBPII-based flow cytometric assay confirmed that DBPII-BIAb activity was associated with the breadth rather than the magnitude of anti-DBPII antibodies. Altogether, our results suggest that multiplex detection of broadly DBPII-reactive antibodies facilitates preliminary screening of BIAb responders.Duffy binding proteinPlasmodium vivaxinhibitory antibodiesmalariamultiplex assayinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/53266/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALMultiplexed Microsphere-Based Flow Cytometric.pdfMultiplexed Microsphere-Based Flow Cytometric.pdfapplication/pdf1300428https://www.arca.fiocruz.br/bitstream/icict/53266/2/Multiplexed%20Microsphere-Based%20Flow%20Cytometric.pdfae4049a0a69db0cbd1a93b2804801376MD52icict/532662022-11-29 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dc.title.pt_BR.fl_str_mv Multiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Responders
title Multiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Responders
spellingShingle Multiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Responders
Alves, Jéssica Rafaela dos Santos
Duffy binding protein
Plasmodium vivax
inhibitory antibodies
malaria
multiplex assay
title_short Multiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Responders
title_full Multiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Responders
title_fullStr Multiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Responders
title_full_unstemmed Multiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Responders
title_sort Multiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Responders
author Alves, Jéssica Rafaela dos Santos
author_facet Alves, Jéssica Rafaela dos Santos
Araújo, Fernanda Fortes de
Pires, Camilla V.
Carvalho, Andréa Teixeira de
Lima, Barbara Andreza Soares
Torres, Letícia de Menezes
Ntumngia, Francis B.
Adams, John H.
Kano, Flora Satiko
Carvalho, Luzia Helena
author_role author
author2 Araújo, Fernanda Fortes de
Pires, Camilla V.
Carvalho, Andréa Teixeira de
Lima, Barbara Andreza Soares
Torres, Letícia de Menezes
Ntumngia, Francis B.
Adams, John H.
Kano, Flora Satiko
Carvalho, Luzia Helena
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alves, Jéssica Rafaela dos Santos
Araújo, Fernanda Fortes de
Pires, Camilla V.
Carvalho, Andréa Teixeira de
Lima, Barbara Andreza Soares
Torres, Letícia de Menezes
Ntumngia, Francis B.
Adams, John H.
Kano, Flora Satiko
Carvalho, Luzia Helena
dc.subject.en.pt_BR.fl_str_mv Duffy binding protein
Plasmodium vivax
inhibitory antibodies
malaria
multiplex assay
topic Duffy binding protein
Plasmodium vivax
inhibitory antibodies
malaria
multiplex assay
description Molecular Biology and Malaria Immunology. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-06-14T17:47:46Z
dc.date.available.fl_str_mv 2022-06-14T17:47:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALVES, Jéssica Rafaela dos Santos et al. Multiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Responders. Front Immunol., v. 12, 704653, 2021. doi: 10.3389/fimmu.2021.704653.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/53266
dc.identifier.issn.pt_BR.fl_str_mv 1664-3224
dc.identifier.doi.none.fl_str_mv 10.3389/fimmu.2021.704653
identifier_str_mv ALVES, Jéssica Rafaela dos Santos et al. Multiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Responders. Front Immunol., v. 12, 704653, 2021. doi: 10.3389/fimmu.2021.704653.
1664-3224
10.3389/fimmu.2021.704653
url https://www.arca.fiocruz.br/handle/icict/53266
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/53266/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/53266/2/Multiplexed%20Microsphere-Based%20Flow%20Cytometric.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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