ELISA for early diagnosis of histoplasmosis.

Detalhes bibliográficos
Autor(a) principal: Guimarães, A. J.
Data de Publicação: 2004
Outros Autores: Pizzini, C. V., Guedes, H. L. M., Albuquerque, P. C., Peralta, J. M., Hamilton, A. J., Oliveira, R. M. Z.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32963
Resumo: 2019-11-06
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spelling Guimarães, A. J.Pizzini, C. V.Guedes, H. L. M.Albuquerque, P. C.Peralta, J. M.Hamilton, A. J.Oliveira, R. M. Z.2019-05-07T02:03:19Z2019-05-07T02:03:19Z2004GUIMARÃES, Allan Jefferson et al. ELISA for early diagnosis of histoplasmosis. Journal of Medical Microbiology, v. 53, p. 509-514, 2004.0095-1137https://www.arca.fiocruz.br/handle/icict/3296310.1099/jmm.0.05469-01098-660XengAmerican Society for MicrobiologyELISA for early diagnosis of histoplasmosis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2019-11-06Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.King’s College. Guy’s Hospital. St John’s Institute of Dermatology. London, UK.An ELISA was developed and evaluated as a method for detecting antibodies against glycosylated and deglycosylated histoplasmin (HMIN). Sera from patients with histoplasmosis, paracoccidioidomycosis, sporotrichosis, coccidioidomycosis, aspergillosis, cryptococcosis and healthy donors were tested by ELISA against purified, deglycosylated histoplasmin (ptHMIN) and compared with purified, native (i.e. glycosylated) histoplasmin (pHMIN). Although cross-reactivity was not abolished when ptHMIN was used in the test, it was reduced (pHMIN ELISA 93 % versus ptHMIN ELISA 96 %). However, there were statistically significant differences between the sensitivities of these two methods for the detection of antibodies (pHMIN ELISA 57 % versus ptHMIN ELISA 92 %; P , 0.001) and between the efficiency of the methods (pHMIN ELISA 83 % versus ptHMIN ELISA 95 %; P , 0.001). These parameters compare better than previously published data relating to the use of treated HMIN in diagnostic ELISAs. Some of the reactivities of serum samples were compared by immunoblotting using deglycosylated HMIN and by immunodiffusion using the crude antigen. The results demonstrated that cross-reactions with heterologous sera in both ELISAs could also be observed in immunoblotting and arose from shared protein epitopes. These data suggest that ELISA using deglycosylated HMIN is a very sensitive diagnostic method and, by using commercially available antigen, it can be easily standardized and performed faster than previousWestern blot-based tests using the same antigen. It provides a useful adjunct to existing methods of diagnosis that could be applied even in situations where laboratory facilities were relatively limited.ELISAHistoplasmosisDiagnosisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/32963/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Guimarães_Allan_etal_INI_2004.pdfve_Guimarães_Allan_etal_INI_2004.pdfapplication/pdf174005https://www.arca.fiocruz.br/bitstream/icict/32963/2/ve_Guimar%c3%a3es_Allan_etal_INI_2004.pdfbd3b084a480a31613b57f5ea878e8644MD52TEXTve_Guimarães_Allan_etal_INI_2004.pdf.txtve_Guimarães_Allan_etal_INI_2004.pdf.txtExtracted texttext/plain30218https://www.arca.fiocruz.br/bitstream/icict/32963/3/ve_Guimar%c3%a3es_Allan_etal_INI_2004.pdf.txt07e11f1cd70fd74c5f5db289b30367b2MD53icict/329632021-03-24 16:32:24.986oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:24Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv ELISA for early diagnosis of histoplasmosis.
title ELISA for early diagnosis of histoplasmosis.
spellingShingle ELISA for early diagnosis of histoplasmosis.
Guimarães, A. J.
ELISA
Histoplasmosis
Diagnosis
title_short ELISA for early diagnosis of histoplasmosis.
title_full ELISA for early diagnosis of histoplasmosis.
title_fullStr ELISA for early diagnosis of histoplasmosis.
title_full_unstemmed ELISA for early diagnosis of histoplasmosis.
title_sort ELISA for early diagnosis of histoplasmosis.
author Guimarães, A. J.
author_facet Guimarães, A. J.
Pizzini, C. V.
Guedes, H. L. M.
Albuquerque, P. C.
Peralta, J. M.
Hamilton, A. J.
Oliveira, R. M. Z.
author_role author
author2 Pizzini, C. V.
Guedes, H. L. M.
Albuquerque, P. C.
Peralta, J. M.
Hamilton, A. J.
Oliveira, R. M. Z.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Guimarães, A. J.
Pizzini, C. V.
Guedes, H. L. M.
Albuquerque, P. C.
Peralta, J. M.
Hamilton, A. J.
Oliveira, R. M. Z.
dc.subject.en.pt_BR.fl_str_mv ELISA
Histoplasmosis
Diagnosis
topic ELISA
Histoplasmosis
Diagnosis
description 2019-11-06
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2019-05-07T02:03:19Z
dc.date.available.fl_str_mv 2019-05-07T02:03:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GUIMARÃES, Allan Jefferson et al. ELISA for early diagnosis of histoplasmosis. Journal of Medical Microbiology, v. 53, p. 509-514, 2004.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32963
dc.identifier.issn.pt_BR.fl_str_mv 0095-1137
dc.identifier.doi.none.fl_str_mv 10.1099/jmm.0.05469-0
dc.identifier.eissn.none.fl_str_mv 1098-660X
identifier_str_mv GUIMARÃES, Allan Jefferson et al. ELISA for early diagnosis of histoplasmosis. Journal of Medical Microbiology, v. 53, p. 509-514, 2004.
0095-1137
10.1099/jmm.0.05469-0
1098-660X
url https://www.arca.fiocruz.br/handle/icict/32963
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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