Flow Cytometry in the Study of Cell Death

Detalhes bibliográficos
Autor(a) principal: Bertho, Álvaro L.
Data de Publicação: 2000
Outros Autores: Santiago, Marta A., Coutinho, Sérgio G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44068
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Laboratório de Imunidade Celular e Humoral. Rio de Janeiro, RJ, Brasil.
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spelling Bertho, Álvaro L.Santiago, Marta A.Coutinho, Sérgio G.2020-10-19T18:51:06Z2020-10-19T18:51:06Z2000BERTHO, Álvaro L.; SANTIAGO, Marta A.; COUTINHO, Sérgio G. Flow Cytometry in the Study of Cell Death. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 3, p. 429-433, May/June 2000.0074-0276https://www.arca.fiocruz.br/handle/icict/4406810.1590/S0074-027620000003000201678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.ApoptoseCitometria de fluxoMorte celularNecroseApoptosisFlow cytometryCell deathNecrosisFlow Cytometry in the Study of Cell Deathinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Laboratório de Imunidade Celular e Humoral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Laboratório de Imunidade Celular e Humoral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Laboratório de Imunidade Celular e Humoral. Rio de Janeiro, RJ, Brasil.In this report we present a concise review concerning the use of flow cytometric methods to characterize and differentiate between two different mechanisms of cell death, apoptosis and necrosis. The applications of these techniques to clinical and basic research are also considered. The following cell features are useful to characterize the mode of cell death: (1) activation of an endonuclease in apoptotic cells results in extraction of the low molecular weight DNA following cell permeabilization, which, in turn, leads to their decreased stainability with DNA-specific fluorochromes. Measurements of DNA content make it possible to identify apoptotic cells and to recognize the cell cycle phase specificity of apoptotic process; (2) plasma membrane integrity, which is lost in necrotic but not in apoptotic cells; (3) the decrease in forward light scatter, paralleled either by no change or an increase in side scatter, represent early changes during apoptosis. The data presented indicate that flow cytometry can be applied to basic research of the molecular and biochemical mechanisms of apoptosis, as well as in the clinical situations, where the ability to monitor early signs of apoptosis in some systems may be predictive for the outcome of some treatment protocols.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44068/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSergioGCoutinho_AlvaroBertho_etal_IOC_2000.pdfSergioGCoutinho_AlvaroBertho_etal_IOC_2000.pdfapplication/pdf59957https://www.arca.fiocruz.br/bitstream/icict/44068/2/SergioGCoutinho_AlvaroBertho_etal_IOC_2000.pdfb770fc49f9806820162edfb180d5ebb9MD52TEXTSergioGCoutinho_AlvaroBertho_etal_IOC_2000.pdf.txtSergioGCoutinho_AlvaroBertho_etal_IOC_2000.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Flow Cytometry in the Study of Cell Death
title Flow Cytometry in the Study of Cell Death
spellingShingle Flow Cytometry in the Study of Cell Death
Bertho, Álvaro L.
Apoptose
Citometria de fluxo
Morte celular
Necrose
Apoptosis
Flow cytometry
Cell death
Necrosis
title_short Flow Cytometry in the Study of Cell Death
title_full Flow Cytometry in the Study of Cell Death
title_fullStr Flow Cytometry in the Study of Cell Death
title_full_unstemmed Flow Cytometry in the Study of Cell Death
title_sort Flow Cytometry in the Study of Cell Death
author Bertho, Álvaro L.
author_facet Bertho, Álvaro L.
Santiago, Marta A.
Coutinho, Sérgio G.
author_role author
author2 Santiago, Marta A.
Coutinho, Sérgio G.
author2_role author
author
dc.contributor.author.fl_str_mv Bertho, Álvaro L.
Santiago, Marta A.
Coutinho, Sérgio G.
dc.subject.other.pt_BR.fl_str_mv Apoptose
Citometria de fluxo
Morte celular
Necrose
topic Apoptose
Citometria de fluxo
Morte celular
Necrose
Apoptosis
Flow cytometry
Cell death
Necrosis
dc.subject.en.pt_BR.fl_str_mv Apoptosis
Flow cytometry
Cell death
Necrosis
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Laboratório de Imunidade Celular e Humoral. Rio de Janeiro, RJ, Brasil.
publishDate 2000
dc.date.issued.fl_str_mv 2000
dc.date.accessioned.fl_str_mv 2020-10-19T18:51:06Z
dc.date.available.fl_str_mv 2020-10-19T18:51:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv BERTHO, Álvaro L.; SANTIAGO, Marta A.; COUTINHO, Sérgio G. Flow Cytometry in the Study of Cell Death. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 3, p. 429-433, May/June 2000.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44068
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762000000300020
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv BERTHO, Álvaro L.; SANTIAGO, Marta A.; COUTINHO, Sérgio G. Flow Cytometry in the Study of Cell Death. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 3, p. 429-433, May/June 2000.
0074-0276
10.1590/S0074-02762000000300020
1678-8060
url https://www.arca.fiocruz.br/handle/icict/44068
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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