Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs

Detalhes bibliográficos
Autor(a) principal: Penha, Euler Moraes
Data de Publicação: 2014
Outros Autores: Meira, Cássio Santana, Guimarães, Elisalva Teixeira, Mendonça, Marcus Vinícius Pinheiro, Gravely, Faye Alice, Pinheiro, Cláudia Maria Bahia, Pinheiro, Taiana Maria Bahia, Melo, Stella Maria Barrouin, Santos, Ricardo Ribeiro dos, Soares, Milena Botelho Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7764
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital de Medicina Veterinária. Escola de Medicina Veterinária e Zootecnia. Salvador, BA, Brasil
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spelling Penha, Euler MoraesMeira, Cássio SantanaGuimarães, Elisalva TeixeiraMendonça, Marcus Vinícius PinheiroGravely, Faye AlicePinheiro, Cláudia Maria BahiaPinheiro, Taiana Maria BahiaMelo, Stella Maria BarrouinSantos, Ricardo Ribeiro dosSoares, Milena Botelho Pereira2014-06-03T19:00:40Z2014-06-03T19:00:40Z2014PENHA, E. M. et al. Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs. Stem Cells International, p.37521, 2014.1687-966Xhttps://www.arca.fiocruz.br/handle/icict/7764dx.doi.org/10.1155/2014/437521engHindawi Publishing CorporationUse of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital de Medicina Veterinária. Escola de Medicina Veterinária e Zootecnia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil /Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade do Estado da Bahia. Departamento de Ciências da Vida. Salvador, BA, BrasilHospital Espanhol. Salvador, BA, BrasilA Arca Veterinária. Salvador, BA, BrasilEstácio-FIB. Centro Universitário Estácio da Bahia. Salvador, BA, BrasilEstácio-FIB. Centro Universitário Estácio da Bahia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital de Medicina Veterinária. Escola de Medicina Veterinária e Zootecnia. Salvador, BA, BrasilCentro de Biotecnologia e Terapia Celular. Hospital São Rafael. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Centro de Biotecnologia e Terapia Celular. Hospital São Rafael. Salvador, BA, BrasilThe use of stem cells in injury repair has been extensively investigated. Here, we examined the therapeutic effects of autologous bone marrow mesenchymal stem cells (MSC) transplantation in four dogs with natural traumatic spinal cord injuries. MSC were cultured in vitro, and proliferation rate and cell viability were evaluated. Cell suspensions were prepared and surgically administered into the spinal cord. The animals were clinically evaluated and examined by nuclear magnetic resonance. Ten days after the surgical procedure and MSC transplantation, we observed a progressive recovery of the panniculus reflex and diminished superficial and deep pain response, although there were still low proprioceptive reflexes in addition to a hyperreflex in the ataxic hind limb movement responses. Each dog demonstrated an improvement in these gains over time. Conscious reflex recovery occurred simultaneously with moderate improvement in intestine and urinary bladder functions in two of the four dogs. By the 18th month of clinical monitoring, we observed a remarkable clinical amelioration accompanied by improved movement, in three of the four dogs. However, no clinical gain was associated with alterations in magnetic resonance imaging. Our results indicate that MSC are potential candidates for the stem cell therapy following spinal cord injury.Células-troncoMedula ósseaMedula espinhalTransplanteCirurgiaAnimaisCãesRessonância magnéticainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALPenha EM Use of autologous mesenchymal....pdfPenha EM Use of autologous mesenchymal....pdfapplication/pdf2482714https://www.arca.fiocruz.br/bitstream/icict/7764/1/Penha%20EM%20Use%20of%20autologous%20mesenchymal....pdf9305f82a377d77d99f97b37e8abd25d3MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7764/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTPenha EM Use of autologous mesenchymal....pdf.txtPenha EM Use of autologous mesenchymal....pdf.txtExtracted texttext/plain38670https://www.arca.fiocruz.br/bitstream/icict/7764/5/Penha%20EM%20Use%20of%20autologous%20mesenchymal....pdf.txtbc121082864e4cc99ca82dd13c06d0deMD55THUMBNAILPenha EM Use of autologous mesenchymal....pdf.jpgPenha EM Use of autologous mesenchymal....pdf.jpgGenerated Thumbnailimage/jpeg1957https://www.arca.fiocruz.br/bitstream/icict/7764/4/Penha%20EM%20Use%20of%20autologous%20mesenchymal....pdf.jpg88ec26936b63f13056ba7f3416463dd0MD54icict/77642023-03-15 14:33:19.684oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs
title Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs
spellingShingle Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs
Penha, Euler Moraes
Células-tronco
Medula óssea
Medula espinhal
Transplante
Cirurgia
Animais
Cães
Ressonância magnética
title_short Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs
title_full Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs
title_fullStr Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs
title_full_unstemmed Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs
title_sort Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs
author Penha, Euler Moraes
author_facet Penha, Euler Moraes
Meira, Cássio Santana
Guimarães, Elisalva Teixeira
Mendonça, Marcus Vinícius Pinheiro
Gravely, Faye Alice
Pinheiro, Cláudia Maria Bahia
Pinheiro, Taiana Maria Bahia
Melo, Stella Maria Barrouin
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
author_role author
author2 Meira, Cássio Santana
Guimarães, Elisalva Teixeira
Mendonça, Marcus Vinícius Pinheiro
Gravely, Faye Alice
Pinheiro, Cláudia Maria Bahia
Pinheiro, Taiana Maria Bahia
Melo, Stella Maria Barrouin
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Penha, Euler Moraes
Meira, Cássio Santana
Guimarães, Elisalva Teixeira
Mendonça, Marcus Vinícius Pinheiro
Gravely, Faye Alice
Pinheiro, Cláudia Maria Bahia
Pinheiro, Taiana Maria Bahia
Melo, Stella Maria Barrouin
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
dc.subject.decs.pt_BR.fl_str_mv Células-tronco
Medula óssea
Medula espinhal
Transplante
Cirurgia
Animais
Cães
Ressonância magnética
topic Células-tronco
Medula óssea
Medula espinhal
Transplante
Cirurgia
Animais
Cães
Ressonância magnética
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital de Medicina Veterinária. Escola de Medicina Veterinária e Zootecnia. Salvador, BA, Brasil
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-06-03T19:00:40Z
dc.date.available.fl_str_mv 2014-06-03T19:00:40Z
dc.date.issued.fl_str_mv 2014
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PENHA, E. M. et al. Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs. Stem Cells International, p.37521, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7764
dc.identifier.issn.none.fl_str_mv 1687-966X
dc.identifier.doi.none.fl_str_mv dx.doi.org/10.1155/2014/437521
identifier_str_mv PENHA, E. M. et al. Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs. Stem Cells International, p.37521, 2014.
1687-966X
dx.doi.org/10.1155/2014/437521
url https://www.arca.fiocruz.br/handle/icict/7764
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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