Intracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/8082 |
Resumo: | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Imunofarmacologia. Belo Horizonte, MG, Brazil |
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Guerra, Amaral Débora CristinaRachid, Milene AlvarengaVilela, Marcia de CarvalhoCampos, Roberta Dayrell de LimaFerreira, Gustavo PortelaRodrigues, David HenriqueQueiroz, Norinne LacerdaMiranda, Aline Silva deCosta, Vivian VasconcelosCampos, Marco Antônio da SilvaKroon, Erna GeessienTeixeira, Mauro MartinsTeixeira Junior, Antonio Lucio2014-07-25T18:08:36Z2014-07-25T18:08:36Z2011AMARAL, Débora Cristina et al. Intracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice. Journal of Neuroinflammation. 2011; 8: 2310.1186/1742-2094-8-23https://www.arca.fiocruz.br/handle/icict/80821742-2094engBioMed CentralIntracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Imunofarmacologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Imunofarmacologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Imunofarmacologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Imunofarmacologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Imunofarmacologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Imunofarmacologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Imunofarmacologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Imunofarmacologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz.Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Imunofarmacologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Imunofarmacologia. Belo Horizonte, MG, BrazilBackground: Dengue, one of the most important arboviral diseases of humans, may cause severe systemic disease. Although dengue virus (DENV) has been considered to be a non-neurotropic virus, dengue infection hás been associated recently with a series of neurological syndromes, including encephalitis. In this work, we evaluated behavioral changes and inflammatory parameters in C57BL/6 mice infected with non-adapted dengue virus 3 (DENV-3) genotype I. Methods: C57BL/6 mice received 4 × 103 PFU of DENV-3 by an intracranial route. We evaluated the trafficking of leukocytes in brain microvasculature using intravital microscopy, and evaluated chemokine and cytokine profiling by an ELISA test at 3 and 6 days post infection (p.i.). Furthermore, we determined myeloperoxidase activity and immune cell populations, and also performed histopathological analysis and immunostaining for the virus in brain tissue. Results: All animals developed signs of encephalitis and died by day 8 p.i. Motor behavior and muscle tone and strength parameters declined at day 7 p.i. We observed increased leukocyte rolling and adhesion in brain microvasculature of infected mice at days 3 and 6 p.i. The infection was followed by significant increases in IFN-g, TNF-a, CCL2, CCL5, CXCL1, and CXCL2. Histological analysis showed evidence of meningoencephalitis and reactive gliosis. Increased numbers of neutrophils, CD4+ and CD8+ T cells were detected in brain of infected animals, notably at day 6 p.i. Cells immunoreactive for anti-NS-3 were visualized throughout the brain. Conclusion: Intracerebral infection with non-adapted DENV-3 ind uces encephalitis and behavioral changes that precede lethality in mice.Dengue/pathologyMeningoencephalitis/virology MiceMeningoencephalitis/mortalityInbred C57BLinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALIntracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice.pdfIntracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice.pdfapplication/pdf1009998https://www.arca.fiocruz.br/bitstream/icict/8082/1/Intracerebral%20infection%20with%20dengue-3%20virus%20induces%20meningoencephalitis%20and%20behavioral%20changes%20that%20precede%20lethality%20in%20mice.pdf5a58b209c846d4306b8b258af9d55485MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8082/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTIntracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice.pdf.txtIntracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice.pdf.txtExtracted texttext/plain49303https://www.arca.fiocruz.br/bitstream/icict/8082/3/Intracerebral%20infection%20with%20dengue-3%20virus%20induces%20meningoencephalitis%20and%20behavioral%20changes%20that%20precede%20lethality%20in%20mice.pdf.txtffd7ce2226883bcafc27fac06a63e7b5MD53icict/80822018-04-06 08:48:47.464oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:48:47Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Intracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice |
| title |
Intracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice |
| spellingShingle |
Intracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice Guerra, Amaral Débora Cristina Dengue/pathology Meningoencephalitis/virology Mice Meningoencephalitis/mortality Inbred C57BL |
| title_short |
Intracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice |
| title_full |
Intracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice |
| title_fullStr |
Intracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice |
| title_full_unstemmed |
Intracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice |
| title_sort |
Intracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice |
| author |
Guerra, Amaral Débora Cristina |
| author_facet |
Guerra, Amaral Débora Cristina Rachid, Milene Alvarenga Vilela, Marcia de Carvalho Campos, Roberta Dayrell de Lima Ferreira, Gustavo Portela Rodrigues, David Henrique Queiroz, Norinne Lacerda Miranda, Aline Silva de Costa, Vivian Vasconcelos Campos, Marco Antônio da Silva Kroon, Erna Geessien Teixeira, Mauro Martins Teixeira Junior, Antonio Lucio |
| author_role |
author |
| author2 |
Rachid, Milene Alvarenga Vilela, Marcia de Carvalho Campos, Roberta Dayrell de Lima Ferreira, Gustavo Portela Rodrigues, David Henrique Queiroz, Norinne Lacerda Miranda, Aline Silva de Costa, Vivian Vasconcelos Campos, Marco Antônio da Silva Kroon, Erna Geessien Teixeira, Mauro Martins Teixeira Junior, Antonio Lucio |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Guerra, Amaral Débora Cristina Rachid, Milene Alvarenga Vilela, Marcia de Carvalho Campos, Roberta Dayrell de Lima Ferreira, Gustavo Portela Rodrigues, David Henrique Queiroz, Norinne Lacerda Miranda, Aline Silva de Costa, Vivian Vasconcelos Campos, Marco Antônio da Silva Kroon, Erna Geessien Teixeira, Mauro Martins Teixeira Junior, Antonio Lucio |
| dc.subject.en.pt_BR.fl_str_mv |
Dengue/pathology Meningoencephalitis/virology Mice Meningoencephalitis/mortality Inbred C57BL |
| topic |
Dengue/pathology Meningoencephalitis/virology Mice Meningoencephalitis/mortality Inbred C57BL |
| description |
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Imunofarmacologia. Belo Horizonte, MG, Brazil |
| publishDate |
2011 |
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2011 |
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2014-07-25T18:08:36Z |
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2014-07-25T18:08:36Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
AMARAL, Débora Cristina et al. Intracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice. Journal of Neuroinflammation. 2011; 8: 23 |
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https://www.arca.fiocruz.br/handle/icict/8082 |
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10.1186/1742-2094-8-23 |
| dc.identifier.doi.none.fl_str_mv |
1742-2094 |
| identifier_str_mv |
AMARAL, Débora Cristina et al. Intracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice. Journal of Neuroinflammation. 2011; 8: 23 10.1186/1742-2094-8-23 1742-2094 |
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BioMed Central |
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BioMed Central |
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