A influência do ozônio sobre biofilmes de Candida monoespécie e biofilmes mistos de Candida spp./Streptococcus mutans

Detalhes bibliográficos
Autor(a) principal: Higa, Bárbara
Data de Publicação: 2022
Outros Autores: Cintra, Bianca Souza, Álvarez, Carmen Magaly, Ribeiro, Arthur Barcelos, Ferreira, Jair Camargo, Tavares, Denise Crispim, Enriquez, Vanessa, Martinez, Luis R., Pires, Regina Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório do Centro Universitário Braz Cubas
Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/4036
Resumo: This study explores the growth of bacterial, fungal, and interkingdom biofilms under aerobiosis or microaerobic conditions and the effect of ozonated sunflower oil on these biofilms. Candida species and Streptococcus mutans were used to study this interaction due to their importance in oral health and disease as these microorganisms display a synergistic relationship that manifests in the onset of caries and tooth decay. Biofilms were developed in a 96-well microtiter plate at 37ºC for 24 h, under aerobiosis or microaerobic conditions, and treated with ozonated oil for 5 to 120 min. All the microorganisms formed biofilms in both oxygenation conditions. Scanning electron microscopy was used to visualize biofilm morphology. Rodent experiments were performed to verify the oil-related toxicity and its efficacy in oral candidiasis. The growth of all Candida species was increased when co-cultured with S. mutans, whilst the growth of bacterium was greater only when co-cultured with C. krusei and C. orthopsilosis under aerobiosis and microaerobic conditions, respectively. Regardless of the oxygenation condition, ozonated oil significantly reduced the viability of all the tested biofilms and infected mice, showing remarkable microbicidal activity as corroborated with confocal microscopy and minimal toxicity. Thus, ozonated oil therapy can be explored as a strategy to control diseases associated with these biofilms especially in the oral cavity.
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spelling A influência do ozônio sobre biofilmes de Candida monoespécie e biofilmes mistos de Candida spp./Streptococcus mutansOzonated oil is effective at killing Candida species and Streptococcus mutans biofilm-derived cells under aerobic and microaerobic conditionsBiofilmsCandidaOral candidiasisOzoneStreptococcus mutansSunflower oilCNPQ::CIENCIAS DA SAUDE::SAUDE COLETIVAThis study explores the growth of bacterial, fungal, and interkingdom biofilms under aerobiosis or microaerobic conditions and the effect of ozonated sunflower oil on these biofilms. Candida species and Streptococcus mutans were used to study this interaction due to their importance in oral health and disease as these microorganisms display a synergistic relationship that manifests in the onset of caries and tooth decay. Biofilms were developed in a 96-well microtiter plate at 37ºC for 24 h, under aerobiosis or microaerobic conditions, and treated with ozonated oil for 5 to 120 min. All the microorganisms formed biofilms in both oxygenation conditions. Scanning electron microscopy was used to visualize biofilm morphology. Rodent experiments were performed to verify the oil-related toxicity and its efficacy in oral candidiasis. The growth of all Candida species was increased when co-cultured with S. mutans, whilst the growth of bacterium was greater only when co-cultured with C. krusei and C. orthopsilosis under aerobiosis and microaerobic conditions, respectively. Regardless of the oxygenation condition, ozonated oil significantly reduced the viability of all the tested biofilms and infected mice, showing remarkable microbicidal activity as corroborated with confocal microscopy and minimal toxicity. Thus, ozonated oil therapy can be explored as a strategy to control diseases associated with these biofilms especially in the oral cavity.Este estudo explora o crescimento de biofilmes bacterianos, fúngicos e inter-reinos sob condições de aerobiose ou microaeróbicas e o efeito do óleo de girassol ozonizado nesses biofilmes. Espécies de Candida e Streptococcus mutans foram utilizadas para estudar essa interação devido à sua importância na saúde bucal e na doença, pois esses microrganismos apresentam uma relação sinérgica que se manifesta no aparecimento de cáries e cáries dentárias. Os biofilmes foram desenvolvidos em uma placa de microtitulação de 96 poços a 37ºC por 24 h, sob condições de aerobiose ou microaeróbica, e tratados com óleo ozonizado por 5 a 120 min. Todos os microrganismos formaram biofilmes em ambas as condições de oxigenação. A microscopia eletrônica de varredura foi usada para visualizar a morfologia do biofilme. Experimentos com roedores foram realizados para verificar a toxicidade do óleo e sua eficácia na candidíase oral. O crescimento de todas as espécies de Candida foi maior quando co-cultivadas com S. mutans, enquanto o crescimento da bactéria foi maior apenas quando co-cultivadas com C. krusei e C. orthopsilosis em condições de aerobiose e microaeróbica, respectivamente. Independentemente da condição de oxigenação, o óleo ozonizado reduziu significativamente a viabilidade de todos os biofilmes testados e camundongos infectados, apresentando notável atividade microbicida corroborada com microscopia confocal e toxicidade mínima. Assim, a terapia com óleo ozonizado pode ser explorada como estratégia de controle de doenças associadas a esses biofilmes principalmente na cavidade bucal.Universidade de FrancaBrasilUNIFRAN2022-09-22T16:47:08Z2022-09-22T16:47:08Z2022-07-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://repositorio.cruzeirodosul.edu.br/handle/123456789/4036engMedical MycologyHiga, BárbaraCintra, Bianca SouzaÁlvarez, Carmen MagalyRibeiro, Arthur BarcelosFerreira, Jair CamargoTavares, Denise CrispimEnriquez, VanessaMartinez, Luis R.Pires, Regina Helenainfo:eu-repo/semantics/openAccessreponame:Repositório do Centro Universitário Braz Cubasinstname:Centro Universitário Braz Cubas (CUB)instacron:CUB2022-09-22T20:50:44Zoai:repositorio.cruzeirodosul.edu.br:123456789/4036Repositório InstitucionalPUBhttps://repositorio.brazcubas.edu.br/oai/requestbibli@brazcubas.edu.bropendoar:2022-09-22T20:50:44Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)false
dc.title.none.fl_str_mv A influência do ozônio sobre biofilmes de Candida monoespécie e biofilmes mistos de Candida spp./Streptococcus mutans
Ozonated oil is effective at killing Candida species and Streptococcus mutans biofilm-derived cells under aerobic and microaerobic conditions
title A influência do ozônio sobre biofilmes de Candida monoespécie e biofilmes mistos de Candida spp./Streptococcus mutans
spellingShingle A influência do ozônio sobre biofilmes de Candida monoespécie e biofilmes mistos de Candida spp./Streptococcus mutans
Higa, Bárbara
Biofilms
Candida
Oral candidiasis
Ozone
Streptococcus mutans
Sunflower oil
CNPQ::CIENCIAS DA SAUDE::SAUDE COLETIVA
title_short A influência do ozônio sobre biofilmes de Candida monoespécie e biofilmes mistos de Candida spp./Streptococcus mutans
title_full A influência do ozônio sobre biofilmes de Candida monoespécie e biofilmes mistos de Candida spp./Streptococcus mutans
title_fullStr A influência do ozônio sobre biofilmes de Candida monoespécie e biofilmes mistos de Candida spp./Streptococcus mutans
title_full_unstemmed A influência do ozônio sobre biofilmes de Candida monoespécie e biofilmes mistos de Candida spp./Streptococcus mutans
title_sort A influência do ozônio sobre biofilmes de Candida monoespécie e biofilmes mistos de Candida spp./Streptococcus mutans
author Higa, Bárbara
author_facet Higa, Bárbara
Cintra, Bianca Souza
Álvarez, Carmen Magaly
Ribeiro, Arthur Barcelos
Ferreira, Jair Camargo
Tavares, Denise Crispim
Enriquez, Vanessa
Martinez, Luis R.
Pires, Regina Helena
author_role author
author2 Cintra, Bianca Souza
Álvarez, Carmen Magaly
Ribeiro, Arthur Barcelos
Ferreira, Jair Camargo
Tavares, Denise Crispim
Enriquez, Vanessa
Martinez, Luis R.
Pires, Regina Helena
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Higa, Bárbara
Cintra, Bianca Souza
Álvarez, Carmen Magaly
Ribeiro, Arthur Barcelos
Ferreira, Jair Camargo
Tavares, Denise Crispim
Enriquez, Vanessa
Martinez, Luis R.
Pires, Regina Helena
dc.subject.por.fl_str_mv Biofilms
Candida
Oral candidiasis
Ozone
Streptococcus mutans
Sunflower oil
CNPQ::CIENCIAS DA SAUDE::SAUDE COLETIVA
topic Biofilms
Candida
Oral candidiasis
Ozone
Streptococcus mutans
Sunflower oil
CNPQ::CIENCIAS DA SAUDE::SAUDE COLETIVA
description This study explores the growth of bacterial, fungal, and interkingdom biofilms under aerobiosis or microaerobic conditions and the effect of ozonated sunflower oil on these biofilms. Candida species and Streptococcus mutans were used to study this interaction due to their importance in oral health and disease as these microorganisms display a synergistic relationship that manifests in the onset of caries and tooth decay. Biofilms were developed in a 96-well microtiter plate at 37ºC for 24 h, under aerobiosis or microaerobic conditions, and treated with ozonated oil for 5 to 120 min. All the microorganisms formed biofilms in both oxygenation conditions. Scanning electron microscopy was used to visualize biofilm morphology. Rodent experiments were performed to verify the oil-related toxicity and its efficacy in oral candidiasis. The growth of all Candida species was increased when co-cultured with S. mutans, whilst the growth of bacterium was greater only when co-cultured with C. krusei and C. orthopsilosis under aerobiosis and microaerobic conditions, respectively. Regardless of the oxygenation condition, ozonated oil significantly reduced the viability of all the tested biofilms and infected mice, showing remarkable microbicidal activity as corroborated with confocal microscopy and minimal toxicity. Thus, ozonated oil therapy can be explored as a strategy to control diseases associated with these biofilms especially in the oral cavity.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-22T16:47:08Z
2022-09-22T16:47:08Z
2022-07-23
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.cruzeirodosul.edu.br/handle/123456789/4036
url https://repositorio.cruzeirodosul.edu.br/handle/123456789/4036
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Medical Mycology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de Franca
Brasil
UNIFRAN
publisher.none.fl_str_mv Universidade de Franca
Brasil
UNIFRAN
dc.source.none.fl_str_mv reponame:Repositório do Centro Universitário Braz Cubas
instname:Centro Universitário Braz Cubas (CUB)
instacron:CUB
instname_str Centro Universitário Braz Cubas (CUB)
instacron_str CUB
institution CUB
reponame_str Repositório do Centro Universitário Braz Cubas
collection Repositório do Centro Universitário Braz Cubas
repository.name.fl_str_mv Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)
repository.mail.fl_str_mv bibli@brazcubas.edu.br
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