Phenotypic and molecular detection of Salmonella sp. on growing, rearing and production phases in a commercial group of laying hens
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000600503 |
Resumo: | Abstract: This present study was developed with the objective of detect Salmonella sp. by conventional bacteriology and qPCR techniques in samples of flooring material from transport crates (meconium); raising environment (swab of cages and drinking fountains); cloacal swab; food and insects from growing, rearing and production phases in a commercial group of laying hens. A total of 864 samples were collected, among whom 248 originated from growing, 392 from rearing and 224 from production phase. Among the 864 samples, 2,8% where positives in bacteriologic technique and 15.3% in qPCR. Contamination was higher in growing and rearing phases and declined in production phase. Twenty four isolations of Salmonella where typified as Salmonella Agona (41.7%), Salmonella Livingstone (33.3%), Salmonella Cerro (16.7%), Salmonella Senftenberg (4.2%) and Salmonella Schwarzengrund (4.2%). During growing phase Salmonella Livingstone was identified. These findings suggest vertical contamination in the group. During rearing and production phases, isolated materials belong to serovars Agona, Cerro, Senftenberg and Schwarzengrund, pointing to horizontal contamination. It is possible to conclude that both vertical and horizontal contaminations are important during the cycle of commercial egg production and contamination in rearing phase is higher than in growing and production phases. |
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Phenotypic and molecular detection of Salmonella sp. on growing, rearing and production phases in a commercial group of laying hensAlphitobius diaperinusbacteriologycage flooringenvironmentqPCRAbstract: This present study was developed with the objective of detect Salmonella sp. by conventional bacteriology and qPCR techniques in samples of flooring material from transport crates (meconium); raising environment (swab of cages and drinking fountains); cloacal swab; food and insects from growing, rearing and production phases in a commercial group of laying hens. A total of 864 samples were collected, among whom 248 originated from growing, 392 from rearing and 224 from production phase. Among the 864 samples, 2,8% where positives in bacteriologic technique and 15.3% in qPCR. Contamination was higher in growing and rearing phases and declined in production phase. Twenty four isolations of Salmonella where typified as Salmonella Agona (41.7%), Salmonella Livingstone (33.3%), Salmonella Cerro (16.7%), Salmonella Senftenberg (4.2%) and Salmonella Schwarzengrund (4.2%). During growing phase Salmonella Livingstone was identified. These findings suggest vertical contamination in the group. During rearing and production phases, isolated materials belong to serovars Agona, Cerro, Senftenberg and Schwarzengrund, pointing to horizontal contamination. It is possible to conclude that both vertical and horizontal contaminations are important during the cycle of commercial egg production and contamination in rearing phase is higher than in growing and production phases.Colégio Brasileiro de Patologia Animal - CBPA2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000600503Pesquisa Veterinária Brasileira v.36 n.6 2016reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2016000600007info:eu-repo/semantics/openAccessMoraes,Dunya M.C.Andrade,Maria A.Duarte,Sabrina C.Bastos,Thiago S.A.Arnhold,EmmanuelJayme,Valéria de S.Nunes,Iolanda Aparecidaeng2016-08-12T00:00:00Zoai:scielo:S0100-736X2016000600503Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2016-08-12T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Phenotypic and molecular detection of Salmonella sp. on growing, rearing and production phases in a commercial group of laying hens |
title |
Phenotypic and molecular detection of Salmonella sp. on growing, rearing and production phases in a commercial group of laying hens |
spellingShingle |
Phenotypic and molecular detection of Salmonella sp. on growing, rearing and production phases in a commercial group of laying hens Moraes,Dunya M.C. Alphitobius diaperinus bacteriology cage flooring environment qPCR |
title_short |
Phenotypic and molecular detection of Salmonella sp. on growing, rearing and production phases in a commercial group of laying hens |
title_full |
Phenotypic and molecular detection of Salmonella sp. on growing, rearing and production phases in a commercial group of laying hens |
title_fullStr |
Phenotypic and molecular detection of Salmonella sp. on growing, rearing and production phases in a commercial group of laying hens |
title_full_unstemmed |
Phenotypic and molecular detection of Salmonella sp. on growing, rearing and production phases in a commercial group of laying hens |
title_sort |
Phenotypic and molecular detection of Salmonella sp. on growing, rearing and production phases in a commercial group of laying hens |
author |
Moraes,Dunya M.C. |
author_facet |
Moraes,Dunya M.C. Andrade,Maria A. Duarte,Sabrina C. Bastos,Thiago S.A. Arnhold,Emmanuel Jayme,Valéria de S. Nunes,Iolanda Aparecida |
author_role |
author |
author2 |
Andrade,Maria A. Duarte,Sabrina C. Bastos,Thiago S.A. Arnhold,Emmanuel Jayme,Valéria de S. Nunes,Iolanda Aparecida |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Moraes,Dunya M.C. Andrade,Maria A. Duarte,Sabrina C. Bastos,Thiago S.A. Arnhold,Emmanuel Jayme,Valéria de S. Nunes,Iolanda Aparecida |
dc.subject.por.fl_str_mv |
Alphitobius diaperinus bacteriology cage flooring environment qPCR |
topic |
Alphitobius diaperinus bacteriology cage flooring environment qPCR |
description |
Abstract: This present study was developed with the objective of detect Salmonella sp. by conventional bacteriology and qPCR techniques in samples of flooring material from transport crates (meconium); raising environment (swab of cages and drinking fountains); cloacal swab; food and insects from growing, rearing and production phases in a commercial group of laying hens. A total of 864 samples were collected, among whom 248 originated from growing, 392 from rearing and 224 from production phase. Among the 864 samples, 2,8% where positives in bacteriologic technique and 15.3% in qPCR. Contamination was higher in growing and rearing phases and declined in production phase. Twenty four isolations of Salmonella where typified as Salmonella Agona (41.7%), Salmonella Livingstone (33.3%), Salmonella Cerro (16.7%), Salmonella Senftenberg (4.2%) and Salmonella Schwarzengrund (4.2%). During growing phase Salmonella Livingstone was identified. These findings suggest vertical contamination in the group. During rearing and production phases, isolated materials belong to serovars Agona, Cerro, Senftenberg and Schwarzengrund, pointing to horizontal contamination. It is possible to conclude that both vertical and horizontal contaminations are important during the cycle of commercial egg production and contamination in rearing phase is higher than in growing and production phases. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000600503 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000600503 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-736X2016000600007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.36 n.6 2016 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122236033236992 |