Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil

Detalhes bibliográficos
Autor(a) principal: Ferreira, Maicom Vinícios
Data de Publicação: 2022
Outros Autores: Gava, Danielle, Schaefer, Rejane, Pierozan, Ricardo Luis, Zanella, Janice Reis Ciacci
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/27121
Resumo: The objective of this work was to evaluate the infection caused by influenza A virus (IAV) subtypes and its incidence in pig nurseries in the state of Santa Catarina, Brazil. A total of 423 nursery pigs were sampled in 11 farms, and IAV circulation, viral RNA, and antibodies were identified. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) was used to detect viral RNA in nasal swab samples (70.2%) and to subtype 33 viruses, of which 18 (54.5%) from six nurseries were the H3N2 virus, 6 (18.1%) from two nurseries were H1N1pdm, and 9 (27.2%) from three nurseries could not be subtyped. The enzyme-linked immunosorbent assay (ELISA) detected the presence of IAV antibodies (68%), which was confirmed by the hemagglutination inhibition test, revealing a higher prevalence of antibodies for the H3N2 virus (38.0%), followed by H1N1pdm (23.8%) and H1N2 (3.23%). The obtained data showed that 10.3% of the swine reacted to at least two viral antigens. There is a high prevalence of influenza A virus infection in all 11 piglets nurseries sampled by viral RNA and antibody detection. H3N2 and H1N1pdm, in this order, are the most detected viral subtypes in the 11 sampled nurseries.
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spelling Influenza A virus circulation in pig nurseries in the state of Santa Catarina, BrazilCirculação de vírus influenza A em creches suínas no estado de Santa Catarina, BrasilSus scrofa domesticus; respiratory diseases; viral diseases; zoonosis.Sus scrofa domesticus; doenças respiratórias; doenças virais; zoonose.The objective of this work was to evaluate the infection caused by influenza A virus (IAV) subtypes and its incidence in pig nurseries in the state of Santa Catarina, Brazil. A total of 423 nursery pigs were sampled in 11 farms, and IAV circulation, viral RNA, and antibodies were identified. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) was used to detect viral RNA in nasal swab samples (70.2%) and to subtype 33 viruses, of which 18 (54.5%) from six nurseries were the H3N2 virus, 6 (18.1%) from two nurseries were H1N1pdm, and 9 (27.2%) from three nurseries could not be subtyped. The enzyme-linked immunosorbent assay (ELISA) detected the presence of IAV antibodies (68%), which was confirmed by the hemagglutination inhibition test, revealing a higher prevalence of antibodies for the H3N2 virus (38.0%), followed by H1N1pdm (23.8%) and H1N2 (3.23%). The obtained data showed that 10.3% of the swine reacted to at least two viral antigens. There is a high prevalence of influenza A virus infection in all 11 piglets nurseries sampled by viral RNA and antibody detection. H3N2 and H1N1pdm, in this order, are the most detected viral subtypes in the 11 sampled nurseries.O objetivo deste trabalho foi avaliar a infecção por subtipos do vírus influenza A (IAV) e a sua incidência em creches de suínos, no estado de Santa Catarina, Brasil. Foram amostrados 423 leitões de creche em 11 granjas, e foram identificados circulação de IAV, RNA viral e anticorpos. A transcriptase reversa seguida de quantificação por reação em cadeia da polimerase (RT-qPCR) foi utilizada para detectar o RNA viral em amostras de swab nasal (70,2%) e para subtipar 33 vírus, dos quais 18 (54,5%) de seis creches eram o vírus H3N2, 6 (18,1%) de duas creches eram o H1N1pdm e 9 (27,2%) de três creches não puderam ser subtipificados. O ensaio de imunoabsorção enzimática (ELISA) detectou a presença de anticorpos IAV (68%), a qual foi confirmada pelo teste de inibição da hemaglutinação, tendo revelado maior prevalência de anticorpos para o vírus H3N2 (38,0%), seguido por H1N1pdm (23,8%) e H1N2 (3,23%). Os dados obtidos mostraram que 10,3% dos suínos reagiram a pelo menos dois antígenos virais. Há alta prevalência de infecção pelo vírus influenza A em todas as 11 creches de leitões amostradas por RNA viral e detecção de anticorpos. H3N2 e H1N1pdm, nesta ordem, são os subtipos virais mais detectados nas 11 creches amostradas.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraBRF S.A., Boehringer IngelheimEmbrapaprofessionals of the agricultural development team of BRF S.A. and of the animal health laboratory of Embrapa Swine and Poultry.BRF S.A., Boehringer IngelheimEmbrapaprofissionais da equipe de desenvolvimento agropecuário da BRF S.A. e do Laboratório de Sanidade Animal da Embrapa Suínos e Aves.Ferreira, Maicom ViníciosGava, DanielleSchaefer, RejanePierozan, Ricardo LuisZanella, Janice Reis Ciacci2022-09-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/27121Pesquisa Agropecuaria Brasileira; V.57, Jan./Dec., 2022: Publicação contínua em volume anual; e02816Pesquisa Agropecuária Brasileira; V.57, Jan./Dec., 2022: Publicação contínua em volume anual; e028161678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/27121/15061Direitos autorais 2022 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2023-01-23T13:40:23Zoai:ojs.seer.sct.embrapa.br:article/27121Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2023-01-23T13:40:23Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil
Circulação de vírus influenza A em creches suínas no estado de Santa Catarina, Brasil
title Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil
spellingShingle Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil
Ferreira, Maicom Vinícios
Sus scrofa domesticus; respiratory diseases; viral diseases; zoonosis.
Sus scrofa domesticus; doenças respiratórias; doenças virais; zoonose.
title_short Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil
title_full Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil
title_fullStr Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil
title_full_unstemmed Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil
title_sort Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil
author Ferreira, Maicom Vinícios
author_facet Ferreira, Maicom Vinícios
Gava, Danielle
Schaefer, Rejane
Pierozan, Ricardo Luis
Zanella, Janice Reis Ciacci
author_role author
author2 Gava, Danielle
Schaefer, Rejane
Pierozan, Ricardo Luis
Zanella, Janice Reis Ciacci
author2_role author
author
author
author
dc.contributor.none.fl_str_mv BRF S.A., Boehringer Ingelheim
Embrapa
professionals of the agricultural development team of BRF S.A. and of the animal health laboratory of Embrapa Swine and Poultry.
BRF S.A., Boehringer Ingelheim
Embrapa
profissionais da equipe de desenvolvimento agropecuário da BRF S.A. e do Laboratório de Sanidade Animal da Embrapa Suínos e Aves.
dc.contributor.author.fl_str_mv Ferreira, Maicom Vinícios
Gava, Danielle
Schaefer, Rejane
Pierozan, Ricardo Luis
Zanella, Janice Reis Ciacci
dc.subject.por.fl_str_mv Sus scrofa domesticus; respiratory diseases; viral diseases; zoonosis.
Sus scrofa domesticus; doenças respiratórias; doenças virais; zoonose.
topic Sus scrofa domesticus; respiratory diseases; viral diseases; zoonosis.
Sus scrofa domesticus; doenças respiratórias; doenças virais; zoonose.
description The objective of this work was to evaluate the infection caused by influenza A virus (IAV) subtypes and its incidence in pig nurseries in the state of Santa Catarina, Brazil. A total of 423 nursery pigs were sampled in 11 farms, and IAV circulation, viral RNA, and antibodies were identified. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) was used to detect viral RNA in nasal swab samples (70.2%) and to subtype 33 viruses, of which 18 (54.5%) from six nurseries were the H3N2 virus, 6 (18.1%) from two nurseries were H1N1pdm, and 9 (27.2%) from three nurseries could not be subtyped. The enzyme-linked immunosorbent assay (ELISA) detected the presence of IAV antibodies (68%), which was confirmed by the hemagglutination inhibition test, revealing a higher prevalence of antibodies for the H3N2 virus (38.0%), followed by H1N1pdm (23.8%) and H1N2 (3.23%). The obtained data showed that 10.3% of the swine reacted to at least two viral antigens. There is a high prevalence of influenza A virus infection in all 11 piglets nurseries sampled by viral RNA and antibody detection. H3N2 and H1N1pdm, in this order, are the most detected viral subtypes in the 11 sampled nurseries.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-14
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/27121
url https://seer.sct.embrapa.br/index.php/pab/article/view/27121
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/27121/15061
dc.rights.driver.fl_str_mv Direitos autorais 2022 Pesquisa Agropecuária Brasileira
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2022 Pesquisa Agropecuária Brasileira
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; V.57, Jan./Dec., 2022: Publicação contínua em volume anual; e02816
Pesquisa Agropecuária Brasileira; V.57, Jan./Dec., 2022: Publicação contínua em volume anual; e02816
1678-3921
0100-104x
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repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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