Quick method for identification of inoculation nodules, efficiency of N2 fixation and cross inoculation of tropical legumes

Detalhes bibliográficos
Autor(a) principal: Stamford, Newton Pereira
Data de Publicação: 2014
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/13857
Resumo: Experiments using leonard jars, soil pots, and field experiments were conducted to observe cross-inoculation in some tropical legumes, the possibility of using black nodule-producing strains for identification of Rhizobium nodules and also the efficiency of such strains in nitrogen fixation. The tropical legumes used were: glycine (Neotonia wightii), lablab (Lablab purpureus), centro (Centrosema pubescens), tropical kudzu (Pueraria phaseoloides) and calopo (Calopogonium mucunoides), inoculated with black nodule-producing strains and normal strains. In cross-inoculation behavior certain host specificity was observed in centro. Lablab and calopo formed black nodules with all black nodule-producing strains, centro with C-102 and CM-7, and glycine produced no black nodules. Strains showed differences in nitrogen fixation efficiency and, in a general way, black nodule-producing strains showed highest efficiency in nitrogen fixation. Results indicated that the black nodule-producing strains may be used in Rhizobium strain identification. 
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spelling Quick method for identification of inoculation nodules, efficiency of N2 fixation and cross inoculation of tropical legumesMétodo rápido de identificação dos nódulos produzidos pelo inoculante, eficiência na fixação do N2 e inoculação cruzada em leguminosas tropicaisRhizobium identification; black nodule strains; Centrosema pubescens; tropical kudzuidentificação de Rhizobium; nódulos pretos; estirpes; Centrosema pubescens; kudzu tropicalExperiments using leonard jars, soil pots, and field experiments were conducted to observe cross-inoculation in some tropical legumes, the possibility of using black nodule-producing strains for identification of Rhizobium nodules and also the efficiency of such strains in nitrogen fixation. The tropical legumes used were: glycine (Neotonia wightii), lablab (Lablab purpureus), centro (Centrosema pubescens), tropical kudzu (Pueraria phaseoloides) and calopo (Calopogonium mucunoides), inoculated with black nodule-producing strains and normal strains. In cross-inoculation behavior certain host specificity was observed in centro. Lablab and calopo formed black nodules with all black nodule-producing strains, centro with C-102 and CM-7, and glycine produced no black nodules. Strains showed differences in nitrogen fixation efficiency and, in a general way, black nodule-producing strains showed highest efficiency in nitrogen fixation. Results indicated that the black nodule-producing strains may be used in Rhizobium strain identification. Foram conduzidos experimentos em vasos-de-leonard, em vasos com solo, e em campo, para verificar a inoculação cruzada, o uso de estirpes formadoras de nódulos pretos na identificação do Rhizobium do inoculante, e sua eficiência na fixação do N2. Foram usadas as leguminosas labe-labe (Lablab purpureus), kudzu tropical (Pueraria phaseoloides), calopogônio (Calopogonium mucunoides), centrosema (Centrosema pubescens), e soja perene (Neotonia wightii), inoculadas com estirpes formadoras de nódulos pretos e de nódulos de coloração normal. Na inoculação cruzada, centrosema mostrou maior grau de especificidade hospedeira. Labe-labe e calopogônio formaram nódulos pretos com todas as estirpes formadoras de nódulos pretos, centrosema e kudzu tropical com as estirpes C-102 e CM-7, e soja perene não apresentou nódulos com esta característica. As estirpes apresentaram diferença na eficiência da fixação do N2 com as estirpes formadoras de nódulos pretos, mostrando-se, em geral, mais eficazes. Os resultados indicaram que as estirpes formadoras de nódulos pretos podem ser usadas para identificar os nódulos produzidos pelo Rhizobium do inoculante. Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraStamford, Newton Pereira2014-04-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/13857Pesquisa Agropecuaria Brasileira; v.23, n.4, abr. 1988; 357-362Pesquisa Agropecuária Brasileira; v.23, n.4, abr. 1988; 357-3621678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/13857/7861info:eu-repo/semantics/openAccess2014-09-11T17:38:34Zoai:ojs.seer.sct.embrapa.br:article/13857Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-09-11T17:38:34Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Quick method for identification of inoculation nodules, efficiency of N2 fixation and cross inoculation of tropical legumes
Método rápido de identificação dos nódulos produzidos pelo inoculante, eficiência na fixação do N2 e inoculação cruzada em leguminosas tropicais
title Quick method for identification of inoculation nodules, efficiency of N2 fixation and cross inoculation of tropical legumes
spellingShingle Quick method for identification of inoculation nodules, efficiency of N2 fixation and cross inoculation of tropical legumes
Stamford, Newton Pereira
Rhizobium identification; black nodule strains; Centrosema pubescens; tropical kudzu
identificação de Rhizobium; nódulos pretos; estirpes; Centrosema pubescens; kudzu tropical
title_short Quick method for identification of inoculation nodules, efficiency of N2 fixation and cross inoculation of tropical legumes
title_full Quick method for identification of inoculation nodules, efficiency of N2 fixation and cross inoculation of tropical legumes
title_fullStr Quick method for identification of inoculation nodules, efficiency of N2 fixation and cross inoculation of tropical legumes
title_full_unstemmed Quick method for identification of inoculation nodules, efficiency of N2 fixation and cross inoculation of tropical legumes
title_sort Quick method for identification of inoculation nodules, efficiency of N2 fixation and cross inoculation of tropical legumes
author Stamford, Newton Pereira
author_facet Stamford, Newton Pereira
author_role author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Stamford, Newton Pereira
dc.subject.por.fl_str_mv Rhizobium identification; black nodule strains; Centrosema pubescens; tropical kudzu
identificação de Rhizobium; nódulos pretos; estirpes; Centrosema pubescens; kudzu tropical
topic Rhizobium identification; black nodule strains; Centrosema pubescens; tropical kudzu
identificação de Rhizobium; nódulos pretos; estirpes; Centrosema pubescens; kudzu tropical
description Experiments using leonard jars, soil pots, and field experiments were conducted to observe cross-inoculation in some tropical legumes, the possibility of using black nodule-producing strains for identification of Rhizobium nodules and also the efficiency of such strains in nitrogen fixation. The tropical legumes used were: glycine (Neotonia wightii), lablab (Lablab purpureus), centro (Centrosema pubescens), tropical kudzu (Pueraria phaseoloides) and calopo (Calopogonium mucunoides), inoculated with black nodule-producing strains and normal strains. In cross-inoculation behavior certain host specificity was observed in centro. Lablab and calopo formed black nodules with all black nodule-producing strains, centro with C-102 and CM-7, and glycine produced no black nodules. Strains showed differences in nitrogen fixation efficiency and, in a general way, black nodule-producing strains showed highest efficiency in nitrogen fixation. Results indicated that the black nodule-producing strains may be used in Rhizobium strain identification. 
publishDate 2014
dc.date.none.fl_str_mv 2014-04-17
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/13857
url https://seer.sct.embrapa.br/index.php/pab/article/view/13857
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/13857/7861
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.23, n.4, abr. 1988; 357-362
Pesquisa Agropecuária Brasileira; v.23, n.4, abr. 1988; 357-362
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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