Benefits of plant cell suspension to vesicular-arbuscular mycorrhizal fungi in vitro. I. Effects of plant species and cell suspension age.

Detalhes bibliográficos
Autor(a) principal: Paula, Mauro Augusto de
Data de Publicação: 2014
Outros Autores: Siqueira, José Oswaldo, Pinto, José Eduardo Brasil Pereira, Pasqual, Moacir
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/13567
Resumo: The effects of addition of cell suspension on germination, rate and micelial growth of vesicular-arbuscular mycorrhizal fungi spores in vitro were studied, aiming to establish monoxenous culture and sporulation of these fungi in the absence of living roots. Application of lucerne, potato, sweet potato, pueraria and tomato plant cells into agar-plates containing desinfested azygospores of Gigaspora margarita, Gigaspora gigantea and Scutellospora heterogaina, increased azygospore germination and favoured their gemi tubo growth. The beneficial effects were maximum when six-day old pueraria cells were utilized. These cells stimulated micelial growth by as much as 200%, over the no cells control However, neither hyphal growth was directed towards the plant cells nor visual signs of cell colonization by the hyphae or fungal sporulation were observed.
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spelling Benefits of plant cell suspension to vesicular-arbuscular mycorrhizal fungi in vitro. I. Effects of plant species and cell suspension age.Benefícios da suspensão de células vegetais para os fungos micorrízicos vesículo-arbusculares in vitro. I. Efeito da espécie vegetal e da idade das células.micelial growth; spores; monoxenous cultura; absent living roots; azygosporescrescimento micelial; esporos; cultura monoxênica; ausência de raízes vivas; azigósporos.The effects of addition of cell suspension on germination, rate and micelial growth of vesicular-arbuscular mycorrhizal fungi spores in vitro were studied, aiming to establish monoxenous culture and sporulation of these fungi in the absence of living roots. Application of lucerne, potato, sweet potato, pueraria and tomato plant cells into agar-plates containing desinfested azygospores of Gigaspora margarita, Gigaspora gigantea and Scutellospora heterogaina, increased azygospore germination and favoured their gemi tubo growth. The beneficial effects were maximum when six-day old pueraria cells were utilized. These cells stimulated micelial growth by as much as 200%, over the no cells control However, neither hyphal growth was directed towards the plant cells nor visual signs of cell colonization by the hyphae or fungal sporulation were observed.Avaliaram-se os efeitos da adição de suspensão de células de diferentes espécies vegetais sobre a taxa de germinação de esporos e crescimento micelial de fungos micorrízicos vesículo-arbusculares (FMVA), visando o estabelecimento de cultura monoxênica e a esporulação destes fungos na ausência de raízes vivas. A adição de suspensão de células de alfafa, batata, batata-doce, puerária e tomateiro em meio ágar-água, onde azigósporos desinfestados de Gigaspora margarita. Gigaspora gigantea e Scutellospora heterogama foram plaqueados, aumentou a taxa de germinação e o crescimento micelial. Esses efeitos foram máximos quando se utilizou suspensão de células de puerária, com seis dias de idade. A presença de células promoveu crescimento abundante e mais prolongado, exercendo estímulos de até 200%. O crescimento do tubo germinativo, embora estimulado, não foi direcionado para as células vegetais, como também não foram verificados indícios de colonização destas pelas hifas do fungo, nem observados sinais visuais de esporulação.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraPaula, Mauro Augusto deSiqueira, José OswaldoPinto, José Eduardo Brasil PereiraPasqual, Moacir2014-04-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/13567Pesquisa Agropecuaria Brasileira; v.25, n.8, ago. 1990; 1101-1108Pesquisa Agropecuária Brasileira; v.25, n.8, ago. 1990; 1101-11081678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/13567/7701info:eu-repo/semantics/openAccess2014-04-17T14:39:56Zoai:ojs.seer.sct.embrapa.br:article/13567Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-04-17T14:39:56Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Benefits of plant cell suspension to vesicular-arbuscular mycorrhizal fungi in vitro. I. Effects of plant species and cell suspension age.
Benefícios da suspensão de células vegetais para os fungos micorrízicos vesículo-arbusculares in vitro. I. Efeito da espécie vegetal e da idade das células.
title Benefits of plant cell suspension to vesicular-arbuscular mycorrhizal fungi in vitro. I. Effects of plant species and cell suspension age.
spellingShingle Benefits of plant cell suspension to vesicular-arbuscular mycorrhizal fungi in vitro. I. Effects of plant species and cell suspension age.
Paula, Mauro Augusto de
micelial growth; spores; monoxenous cultura; absent living roots; azygospores
crescimento micelial; esporos; cultura monoxênica; ausência de raízes vivas; azigósporos.
title_short Benefits of plant cell suspension to vesicular-arbuscular mycorrhizal fungi in vitro. I. Effects of plant species and cell suspension age.
title_full Benefits of plant cell suspension to vesicular-arbuscular mycorrhizal fungi in vitro. I. Effects of plant species and cell suspension age.
title_fullStr Benefits of plant cell suspension to vesicular-arbuscular mycorrhizal fungi in vitro. I. Effects of plant species and cell suspension age.
title_full_unstemmed Benefits of plant cell suspension to vesicular-arbuscular mycorrhizal fungi in vitro. I. Effects of plant species and cell suspension age.
title_sort Benefits of plant cell suspension to vesicular-arbuscular mycorrhizal fungi in vitro. I. Effects of plant species and cell suspension age.
author Paula, Mauro Augusto de
author_facet Paula, Mauro Augusto de
Siqueira, José Oswaldo
Pinto, José Eduardo Brasil Pereira
Pasqual, Moacir
author_role author
author2 Siqueira, José Oswaldo
Pinto, José Eduardo Brasil Pereira
Pasqual, Moacir
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Paula, Mauro Augusto de
Siqueira, José Oswaldo
Pinto, José Eduardo Brasil Pereira
Pasqual, Moacir
dc.subject.por.fl_str_mv micelial growth; spores; monoxenous cultura; absent living roots; azygospores
crescimento micelial; esporos; cultura monoxênica; ausência de raízes vivas; azigósporos.
topic micelial growth; spores; monoxenous cultura; absent living roots; azygospores
crescimento micelial; esporos; cultura monoxênica; ausência de raízes vivas; azigósporos.
description The effects of addition of cell suspension on germination, rate and micelial growth of vesicular-arbuscular mycorrhizal fungi spores in vitro were studied, aiming to establish monoxenous culture and sporulation of these fungi in the absence of living roots. Application of lucerne, potato, sweet potato, pueraria and tomato plant cells into agar-plates containing desinfested azygospores of Gigaspora margarita, Gigaspora gigantea and Scutellospora heterogaina, increased azygospore germination and favoured their gemi tubo growth. The beneficial effects were maximum when six-day old pueraria cells were utilized. These cells stimulated micelial growth by as much as 200%, over the no cells control However, neither hyphal growth was directed towards the plant cells nor visual signs of cell colonization by the hyphae or fungal sporulation were observed.
publishDate 2014
dc.date.none.fl_str_mv 2014-04-17
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/13567
url https://seer.sct.embrapa.br/index.php/pab/article/view/13567
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/13567/7701
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.25, n.8, ago. 1990; 1101-1108
Pesquisa Agropecuária Brasileira; v.25, n.8, ago. 1990; 1101-1108
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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