DNA barcodes for soil animal taxonomy
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/1523 |
Resumo: | The biodiversity of soil communities remains very poorly known and understood. Soil biological sciences are strongly affected by the taxonomic crisis, and most groups of animals in that biota suffer from a strong taxonomic impediment. The objective of this work was to investigate how DNA barcoding – a novel method using a microgenomic tag for species identification and discrimination – permits better evaluation of the taxonomy of soil biota. A total of 1,152 barcode sequences were analyzed for two major groups of animals, collembolans and earthworms, which presented broad taxonomic and geographic sampling. Besides strongly reflecting the taxonomic impediment for both groups, with a large number of species-level divergent lineages remaining unnamed so far, the results also highlight a high level (15%) of cryptic diversity within known species of both earthworms and collembolans. These results are supportive of recent local studies using a similar approach. Within an impeded taxonomic system for soil animals, DNA-assisted identification tools can facilitate and improve biodiversity exploration and description. DNA-barcoding campaigns are rapidly developing in soil animals and the community of soil biologists is urged to embrace these methods. |
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DNA barcodes for soil animal taxonomyCódigo de barras de DNA para a taxonomia de animais do soloCollembola; cryptic diversity; DNA barcoding; Oligochaeta; species identification; taxonomic crisis; taxonomic impedimentCollembola; diversidade críptica; código de barras de DNA; Oligochaeta; identificação de espécies; crise taxonômica; impedimento taxonômicoThe biodiversity of soil communities remains very poorly known and understood. Soil biological sciences are strongly affected by the taxonomic crisis, and most groups of animals in that biota suffer from a strong taxonomic impediment. The objective of this work was to investigate how DNA barcoding – a novel method using a microgenomic tag for species identification and discrimination – permits better evaluation of the taxonomy of soil biota. A total of 1,152 barcode sequences were analyzed for two major groups of animals, collembolans and earthworms, which presented broad taxonomic and geographic sampling. Besides strongly reflecting the taxonomic impediment for both groups, with a large number of species-level divergent lineages remaining unnamed so far, the results also highlight a high level (15%) of cryptic diversity within known species of both earthworms and collembolans. These results are supportive of recent local studies using a similar approach. Within an impeded taxonomic system for soil animals, DNA-assisted identification tools can facilitate and improve biodiversity exploration and description. DNA-barcoding campaigns are rapidly developing in soil animals and the community of soil biologists is urged to embrace these methods. A biodiversidade das comunidades do solo continua muito pouco conhecida e entendida. A biologia do solo é fortemente afetada pela crise taxonômica, e a maior parte dos grupos de animais dessa biota sofre forte impedimento taxonômico. O objetivo deste trabalho foi determinar como o código de barras de DNA – um método novo que usa uma etiqueta microgenômica para identificação e discriminação de espécies – permite uma melhor avaliação da taxonomia da biota edáfica. Foram analisadas 1.152 sequências de códigos de barras de dois grupos principais de animais, colêmbolos e minhocas, que apresentaram ampla amostragem taxonômica e geográfica. Além de refletir fortemente o impedimento taxonômico de ambos os grupos, com um grande número de linhagens divergentes no nível da espécie que ainda não está descrita, os resultados também destacam um alto (15%) nível de diversidade críptica dentro de espécies conhecidas de minhocas e colêmbolos. Esses resultados apóiam estudos locais recentes que usaram métodos similares. Considerando as dificuldades taxonômicas enfrentadas para identificar os animais de solo, ferramentas de identificação usando DNA podem facilitar e melhorar a exploração da biodiversidade e a sua descrição. As campanhas de código de barras de DNA estão se desenvolvendo rapidamente com animais do solo, e a comunidade de biólogos é incitada a adotar esses métodos. Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraGenome Canada, NSERC, Ontario Genomics Institute, NSFGenome Canada, NSERC, Ontario Genomics Institute, NSFRougerie, RodolpheDecaens, ThibaudDeharveng, LouisPorco, DavidJames, Sam W.Chang, Chih-HanRichard, BenoitPotapov, MikhailSuhardjono, YayukHebert, Paul D. N2010-11-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/1523Pesquisa Agropecuaria Brasileira; v.44, n.8, ago. 2009: Thematic Issue XV International Colloquium on Soil Zoology and XX Colloquium on Apterygota; 789-801Pesquisa Agropecuária Brasileira; v.44, n.8, ago. 2009: Thematic Issue XV International Colloquium on Soil Zoology and XX Colloquium on Apterygota; 789-8011678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/1523/5769https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1523/2168https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1523/2324https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1523/2325info:eu-repo/semantics/openAccess2012-06-17T11:52:04Zoai:ojs.seer.sct.embrapa.br:article/1523Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T11:52:04Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
DNA barcodes for soil animal taxonomy Código de barras de DNA para a taxonomia de animais do solo |
title |
DNA barcodes for soil animal taxonomy |
spellingShingle |
DNA barcodes for soil animal taxonomy Rougerie, Rodolphe Collembola; cryptic diversity; DNA barcoding; Oligochaeta; species identification; taxonomic crisis; taxonomic impediment Collembola; diversidade críptica; código de barras de DNA; Oligochaeta; identificação de espécies; crise taxonômica; impedimento taxonômico |
title_short |
DNA barcodes for soil animal taxonomy |
title_full |
DNA barcodes for soil animal taxonomy |
title_fullStr |
DNA barcodes for soil animal taxonomy |
title_full_unstemmed |
DNA barcodes for soil animal taxonomy |
title_sort |
DNA barcodes for soil animal taxonomy |
author |
Rougerie, Rodolphe |
author_facet |
Rougerie, Rodolphe Decaens, Thibaud Deharveng, Louis Porco, David James, Sam W. Chang, Chih-Han Richard, Benoit Potapov, Mikhail Suhardjono, Yayuk Hebert, Paul D. N |
author_role |
author |
author2 |
Decaens, Thibaud Deharveng, Louis Porco, David James, Sam W. Chang, Chih-Han Richard, Benoit Potapov, Mikhail Suhardjono, Yayuk Hebert, Paul D. N |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Genome Canada, NSERC, Ontario Genomics Institute, NSF Genome Canada, NSERC, Ontario Genomics Institute, NSF |
dc.contributor.author.fl_str_mv |
Rougerie, Rodolphe Decaens, Thibaud Deharveng, Louis Porco, David James, Sam W. Chang, Chih-Han Richard, Benoit Potapov, Mikhail Suhardjono, Yayuk Hebert, Paul D. N |
dc.subject.por.fl_str_mv |
Collembola; cryptic diversity; DNA barcoding; Oligochaeta; species identification; taxonomic crisis; taxonomic impediment Collembola; diversidade críptica; código de barras de DNA; Oligochaeta; identificação de espécies; crise taxonômica; impedimento taxonômico |
topic |
Collembola; cryptic diversity; DNA barcoding; Oligochaeta; species identification; taxonomic crisis; taxonomic impediment Collembola; diversidade críptica; código de barras de DNA; Oligochaeta; identificação de espécies; crise taxonômica; impedimento taxonômico |
description |
The biodiversity of soil communities remains very poorly known and understood. Soil biological sciences are strongly affected by the taxonomic crisis, and most groups of animals in that biota suffer from a strong taxonomic impediment. The objective of this work was to investigate how DNA barcoding – a novel method using a microgenomic tag for species identification and discrimination – permits better evaluation of the taxonomy of soil biota. A total of 1,152 barcode sequences were analyzed for two major groups of animals, collembolans and earthworms, which presented broad taxonomic and geographic sampling. Besides strongly reflecting the taxonomic impediment for both groups, with a large number of species-level divergent lineages remaining unnamed so far, the results also highlight a high level (15%) of cryptic diversity within known species of both earthworms and collembolans. These results are supportive of recent local studies using a similar approach. Within an impeded taxonomic system for soil animals, DNA-assisted identification tools can facilitate and improve biodiversity exploration and description. DNA-barcoding campaigns are rapidly developing in soil animals and the community of soil biologists is urged to embrace these methods. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-11-18 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/1523 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/1523 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/1523/5769 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1523/2168 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1523/2324 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1523/2325 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.44, n.8, ago. 2009: Thematic Issue XV International Colloquium on Soil Zoology and XX Colloquium on Apterygota; 789-801 Pesquisa Agropecuária Brasileira; v.44, n.8, ago. 2009: Thematic Issue XV International Colloquium on Soil Zoology and XX Colloquium on Apterygota; 789-801 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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