Diet crude protein reduction on follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1146377 https://doi.org/10.1590/1984-3143-AR2021-0088 |
Resumo: | This study evaluated the effect of crude protein (CP) reduction in four diets (156, 139, 132, and 127 g Kg-1 DM) maintaining constant metabolizable protein (188 g/day) on the follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows. Twenty-two Girolando cows with average of 21.55 ±3.19 L daily milk yield, 105.30 ±22.62 days in lactation and 3.22 ±0.03 body condition score were selected. To reduce CP in diets and maintain constant metabolizable protein, urea and soybean meal were gradually replaced by lignosulfonate-treated soybean meal (SoyPass®, Cargill), resulting in an increase in rumenundegradable protein and a reduction in rumen degradable protein. A linear and quadratic reduction was observed in the plasma and follicular fluid urea nitrogen concentration following CP reduction, with the most intense reduction occurring in the 127 g Kg-1 DM group (p<0.001). As CP reduced, there was a tendency for a linear increase in the follicular growth rate (P=0.0696), on the number and proportion of viable oocytes (P<0.09), and also a linear increase for the number (P=0.0397) and proportion (P<0.09) of grade I viable oocytes. Plus, there was a linear effect for the number of cumulus oophorus cells. Cows fed with the lowest amount of CP had cumulus-oocyte complexes with higher numbers of cumulus oophorus cells (P=0.0238). Also, the reduction of diet crude protein was followed by a decrease in the probability of oocytes? DNA degradation. In conclusion, the reduction of CP in the diet of mid-lactating Girolando cows, reduces urea nitrogen concentration in both blood plasma and follicular fluid, and, as a consequence, increases the viability of oocytes and the number of cumulus oophorus cells while reducing oocytes? DNA degradation of follicular included cumulus-oocyte complex. The reduction on dietary CP may improve in vivo oocytes-embryo development impacting fertility of lactating dairy cows. |
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Diet crude protein reduction on follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows.ApoptoseBovinoGado LeiteiroNutrição AnimalReprodução AnimalUréiaApoptosisAnimal nutritionAnimal reproductionUreaThis study evaluated the effect of crude protein (CP) reduction in four diets (156, 139, 132, and 127 g Kg-1 DM) maintaining constant metabolizable protein (188 g/day) on the follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows. Twenty-two Girolando cows with average of 21.55 ±3.19 L daily milk yield, 105.30 ±22.62 days in lactation and 3.22 ±0.03 body condition score were selected. To reduce CP in diets and maintain constant metabolizable protein, urea and soybean meal were gradually replaced by lignosulfonate-treated soybean meal (SoyPass®, Cargill), resulting in an increase in rumenundegradable protein and a reduction in rumen degradable protein. A linear and quadratic reduction was observed in the plasma and follicular fluid urea nitrogen concentration following CP reduction, with the most intense reduction occurring in the 127 g Kg-1 DM group (p<0.001). As CP reduced, there was a tendency for a linear increase in the follicular growth rate (P=0.0696), on the number and proportion of viable oocytes (P<0.09), and also a linear increase for the number (P=0.0397) and proportion (P<0.09) of grade I viable oocytes. Plus, there was a linear effect for the number of cumulus oophorus cells. Cows fed with the lowest amount of CP had cumulus-oocyte complexes with higher numbers of cumulus oophorus cells (P=0.0238). Also, the reduction of diet crude protein was followed by a decrease in the probability of oocytes? DNA degradation. In conclusion, the reduction of CP in the diet of mid-lactating Girolando cows, reduces urea nitrogen concentration in both blood plasma and follicular fluid, and, as a consequence, increases the viability of oocytes and the number of cumulus oophorus cells while reducing oocytes? DNA degradation of follicular included cumulus-oocyte complex. The reduction on dietary CP may improve in vivo oocytes-embryo development impacting fertility of lactating dairy cows.LUCIANO DE REZENDE CARVALHEIRA, Universidade Federal Fluminense; GUSTAVO BERVIAN DOS SANTOS, Universidade Federal Fluminense; JASMIM, Universidade Federal do Rio de Janeiro; CLÓVIS RIBEIRO GUIMARÃES, Universidade Federal de Minas Gerais; MARIANA MAGALHAES CAMPOS, CNPGL; FERNANDA SAMARINI MACHADO, CNPGL; ALEXANDRE MENDONÇA PEDROSO, AMP Consultoria; TADEU EDER DA SILVA, University of Wisconsin-Madison; LUIZ ALTAMIRO GARCIA NOGUEIRA, Universidade Federal Fluminense; ANDRÉ LUÍS RIOS RODRIGUES, Universidade Federal Fluminense; BRUNO CAMPOS DE CARVALHO, CNPGL.CARVALHEIRA, L. de R.SANTOS, G. B. dosJASMIMGUIMARÃES, C. R.CAMPOS, M. M.MACHADO, F. S.PEDROSO, A. M.SILVA, T. E. daNOGUEIRA, L. A. G.RODRIGUES, A. L. R.CARVALHO, B. C. de2022-09-13T14:05:39Z2022-09-13T14:05:39Z2022-09-132022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnimal Reproduction, v. 19, n. 3, e20210088, 2022.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1146377https://doi.org/10.1590/1984-3143-AR2021-0088enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2022-09-13T14:05:48Zoai:www.alice.cnptia.embrapa.br:doc/1146377Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542022-09-13T14:05:48falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542022-09-13T14:05:48Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Diet crude protein reduction on follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows. |
title |
Diet crude protein reduction on follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows. |
spellingShingle |
Diet crude protein reduction on follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows. CARVALHEIRA, L. de R. Apoptose Bovino Gado Leiteiro Nutrição Animal Reprodução Animal Uréia Apoptosis Animal nutrition Animal reproduction Urea |
title_short |
Diet crude protein reduction on follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows. |
title_full |
Diet crude protein reduction on follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows. |
title_fullStr |
Diet crude protein reduction on follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows. |
title_full_unstemmed |
Diet crude protein reduction on follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows. |
title_sort |
Diet crude protein reduction on follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows. |
author |
CARVALHEIRA, L. de R. |
author_facet |
CARVALHEIRA, L. de R. SANTOS, G. B. dos JASMIM GUIMARÃES, C. R. CAMPOS, M. M. MACHADO, F. S. PEDROSO, A. M. SILVA, T. E. da NOGUEIRA, L. A. G. RODRIGUES, A. L. R. CARVALHO, B. C. de |
author_role |
author |
author2 |
SANTOS, G. B. dos JASMIM GUIMARÃES, C. R. CAMPOS, M. M. MACHADO, F. S. PEDROSO, A. M. SILVA, T. E. da NOGUEIRA, L. A. G. RODRIGUES, A. L. R. CARVALHO, B. C. de |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
LUCIANO DE REZENDE CARVALHEIRA, Universidade Federal Fluminense; GUSTAVO BERVIAN DOS SANTOS, Universidade Federal Fluminense; JASMIM, Universidade Federal do Rio de Janeiro; CLÓVIS RIBEIRO GUIMARÃES, Universidade Federal de Minas Gerais; MARIANA MAGALHAES CAMPOS, CNPGL; FERNANDA SAMARINI MACHADO, CNPGL; ALEXANDRE MENDONÇA PEDROSO, AMP Consultoria; TADEU EDER DA SILVA, University of Wisconsin-Madison; LUIZ ALTAMIRO GARCIA NOGUEIRA, Universidade Federal Fluminense; ANDRÉ LUÍS RIOS RODRIGUES, Universidade Federal Fluminense; BRUNO CAMPOS DE CARVALHO, CNPGL. |
dc.contributor.author.fl_str_mv |
CARVALHEIRA, L. de R. SANTOS, G. B. dos JASMIM GUIMARÃES, C. R. CAMPOS, M. M. MACHADO, F. S. PEDROSO, A. M. SILVA, T. E. da NOGUEIRA, L. A. G. RODRIGUES, A. L. R. CARVALHO, B. C. de |
dc.subject.por.fl_str_mv |
Apoptose Bovino Gado Leiteiro Nutrição Animal Reprodução Animal Uréia Apoptosis Animal nutrition Animal reproduction Urea |
topic |
Apoptose Bovino Gado Leiteiro Nutrição Animal Reprodução Animal Uréia Apoptosis Animal nutrition Animal reproduction Urea |
description |
This study evaluated the effect of crude protein (CP) reduction in four diets (156, 139, 132, and 127 g Kg-1 DM) maintaining constant metabolizable protein (188 g/day) on the follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows. Twenty-two Girolando cows with average of 21.55 ±3.19 L daily milk yield, 105.30 ±22.62 days in lactation and 3.22 ±0.03 body condition score were selected. To reduce CP in diets and maintain constant metabolizable protein, urea and soybean meal were gradually replaced by lignosulfonate-treated soybean meal (SoyPass®, Cargill), resulting in an increase in rumenundegradable protein and a reduction in rumen degradable protein. A linear and quadratic reduction was observed in the plasma and follicular fluid urea nitrogen concentration following CP reduction, with the most intense reduction occurring in the 127 g Kg-1 DM group (p<0.001). As CP reduced, there was a tendency for a linear increase in the follicular growth rate (P=0.0696), on the number and proportion of viable oocytes (P<0.09), and also a linear increase for the number (P=0.0397) and proportion (P<0.09) of grade I viable oocytes. Plus, there was a linear effect for the number of cumulus oophorus cells. Cows fed with the lowest amount of CP had cumulus-oocyte complexes with higher numbers of cumulus oophorus cells (P=0.0238). Also, the reduction of diet crude protein was followed by a decrease in the probability of oocytes? DNA degradation. In conclusion, the reduction of CP in the diet of mid-lactating Girolando cows, reduces urea nitrogen concentration in both blood plasma and follicular fluid, and, as a consequence, increases the viability of oocytes and the number of cumulus oophorus cells while reducing oocytes? DNA degradation of follicular included cumulus-oocyte complex. The reduction on dietary CP may improve in vivo oocytes-embryo development impacting fertility of lactating dairy cows. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-13T14:05:39Z 2022-09-13T14:05:39Z 2022-09-13 2022 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Animal Reproduction, v. 19, n. 3, e20210088, 2022. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1146377 https://doi.org/10.1590/1984-3143-AR2021-0088 |
identifier_str_mv |
Animal Reproduction, v. 19, n. 3, e20210088, 2022. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1146377 https://doi.org/10.1590/1984-3143-AR2021-0088 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503531096440832 |