Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population.
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/862208 |
Resumo: | A doubled-haploid (DH) population, obtained by anther culture of F 1 plants from a cross between a highly susceptible rice cultivar Lijiangxintuanheigu and the resistant somaclone (SC09), of the cultivar Araguaia, was used to identify RAPD markers linked to the blast resistance gene Pi-ar. The 86 DH plants, inoculated with the race IB-9 of Magnaporthe oryzae, segregated in 1:1 ratio of resistant and susceptible plants. Of the 67 primers used 31 produced DNA profiles that differentiated resistant and susceptible bulks as well as the parental cultivars. The resistance gene was found linked to the primer OPS162072 (?AGGGGGTTCC?) at a distance of 3.6 cM. The selection efficiency of this primer was assessed in a BC3 F1 population derived from another cross between a susceptible cultivar IAC 201 and SC09. The marker OPS16 showed efficiency of 86.9%, when six resistant and two susceptible plants were considered as negatives in RAPD analysis. |
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Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population.Duplo haplóideArrozOryza sativaAnteraBrusoneDoença de plantaMarcador molecularRiceDoubled haploidsAnther cultureMagnaporthe griseaA doubled-haploid (DH) population, obtained by anther culture of F 1 plants from a cross between a highly susceptible rice cultivar Lijiangxintuanheigu and the resistant somaclone (SC09), of the cultivar Araguaia, was used to identify RAPD markers linked to the blast resistance gene Pi-ar. The 86 DH plants, inoculated with the race IB-9 of Magnaporthe oryzae, segregated in 1:1 ratio of resistant and susceptible plants. Of the 67 primers used 31 produced DNA profiles that differentiated resistant and susceptible bulks as well as the parental cultivars. The resistance gene was found linked to the primer OPS162072 (?AGGGGGTTCC?) at a distance of 3.6 cM. The selection efficiency of this primer was assessed in a BC3 F1 population derived from another cross between a susceptible cultivar IAC 201 and SC09. The marker OPS16 showed efficiency of 86.9%, when six resistant and two susceptible plants were considered as negatives in RAPD analysis.LEILA GARCÊS DE ARAÚJO, UFGANNE SITARAMA PRABHU, CNPAFPEDRO ANTONIO ARRAES PEREIRA, CNPAFGISELE BARATA DA SILVA, UFV.ARAÚJO, L. G. dePRABHU, A. S.PEREIRA, P. A. A.SILVA, G. B. da2011-04-09T14:42:41Z2011-04-09T14:42:41Z2010-09-1420102011-04-10T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCrop Breeding and Applied Biotechnology, v. 10, n. 1, p. 23-31, Mar. 2010.http://www.alice.cnptia.embrapa.br/alice/handle/doc/862208enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T21:40:50Zoai:www.alice.cnptia.embrapa.br:doc/862208Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T21:40:50falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T21:40:50Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population. |
title |
Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population. |
spellingShingle |
Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population. ARAÚJO, L. G. de Duplo haplóide Arroz Oryza sativa Antera Brusone Doença de planta Marcador molecular Rice Doubled haploids Anther culture Magnaporthe grisea |
title_short |
Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population. |
title_full |
Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population. |
title_fullStr |
Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population. |
title_full_unstemmed |
Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population. |
title_sort |
Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population. |
author |
ARAÚJO, L. G. de |
author_facet |
ARAÚJO, L. G. de PRABHU, A. S. PEREIRA, P. A. A. SILVA, G. B. da |
author_role |
author |
author2 |
PRABHU, A. S. PEREIRA, P. A. A. SILVA, G. B. da |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
LEILA GARCÊS DE ARAÚJO, UFG ANNE SITARAMA PRABHU, CNPAF PEDRO ANTONIO ARRAES PEREIRA, CNPAF GISELE BARATA DA SILVA, UFV. |
dc.contributor.author.fl_str_mv |
ARAÚJO, L. G. de PRABHU, A. S. PEREIRA, P. A. A. SILVA, G. B. da |
dc.subject.por.fl_str_mv |
Duplo haplóide Arroz Oryza sativa Antera Brusone Doença de planta Marcador molecular Rice Doubled haploids Anther culture Magnaporthe grisea |
topic |
Duplo haplóide Arroz Oryza sativa Antera Brusone Doença de planta Marcador molecular Rice Doubled haploids Anther culture Magnaporthe grisea |
description |
A doubled-haploid (DH) population, obtained by anther culture of F 1 plants from a cross between a highly susceptible rice cultivar Lijiangxintuanheigu and the resistant somaclone (SC09), of the cultivar Araguaia, was used to identify RAPD markers linked to the blast resistance gene Pi-ar. The 86 DH plants, inoculated with the race IB-9 of Magnaporthe oryzae, segregated in 1:1 ratio of resistant and susceptible plants. Of the 67 primers used 31 produced DNA profiles that differentiated resistant and susceptible bulks as well as the parental cultivars. The resistance gene was found linked to the primer OPS162072 (?AGGGGGTTCC?) at a distance of 3.6 cM. The selection efficiency of this primer was assessed in a BC3 F1 population derived from another cross between a susceptible cultivar IAC 201 and SC09. The marker OPS16 showed efficiency of 86.9%, when six resistant and two susceptible plants were considered as negatives in RAPD analysis. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-09-14 2010 2011-04-09T14:42:41Z 2011-04-09T14:42:41Z 2011-04-10T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Crop Breeding and Applied Biotechnology, v. 10, n. 1, p. 23-31, Mar. 2010. http://www.alice.cnptia.embrapa.br/alice/handle/doc/862208 |
identifier_str_mv |
Crop Breeding and Applied Biotechnology, v. 10, n. 1, p. 23-31, Mar. 2010. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/862208 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503321420038144 |