Bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae (Hübner) (Lepidoptera: Pyralidae).
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1041438 |
Resumo: | Biochemical and feeding assays were performed in order to assess the bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae. The biochemical assays were based on the ability of protease inhibitors (PI) present in sesame seeds to inhibit bovine pancreatic trypsin and digestive enzymes of insect larvae. The stability of the inhibitors was also assessed at different pH and temperature. The feeding bioassays were carried out in plates containing second instar larvae fed on diet with sesame protein extract. It was observed that all genotypes presented inhibition when tested with BPT, ranging from 51 to 90%, among them BRS Seda, CNPA G3 and CNPA G4 showed average of 69% of inhibitory activity for intestinal homogenate. The FII-fractions in these three genotypes showed thermal and pH stabilities at 40°C and 8.5, respectively. In feeding bioassay, P. interpunctella larvae were susceptible to SPCE of all three genotypes, revealing average mortality of 74% to SPCE and 45% to FII-fractions. Among all genotypes, BRS Seda was more effective to inhibition of digestive enzymes of insects and considered the more suitable genotype for further using in sesame breeding program to resistance to P. interpunctella. |
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Bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae (Hübner) (Lepidoptera: Pyralidae).Sesamum indicum LTripisinaSeedPlodia interpunctellaGergelimSementeGenótipoLepidopteraBiochemical and feeding assays were performed in order to assess the bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae. The biochemical assays were based on the ability of protease inhibitors (PI) present in sesame seeds to inhibit bovine pancreatic trypsin and digestive enzymes of insect larvae. The stability of the inhibitors was also assessed at different pH and temperature. The feeding bioassays were carried out in plates containing second instar larvae fed on diet with sesame protein extract. It was observed that all genotypes presented inhibition when tested with BPT, ranging from 51 to 90%, among them BRS Seda, CNPA G3 and CNPA G4 showed average of 69% of inhibitory activity for intestinal homogenate. The FII-fractions in these three genotypes showed thermal and pH stabilities at 40°C and 8.5, respectively. In feeding bioassay, P. interpunctella larvae were susceptible to SPCE of all three genotypes, revealing average mortality of 74% to SPCE and 45% to FII-fractions. Among all genotypes, BRS Seda was more effective to inhibition of digestive enzymes of insects and considered the more suitable genotype for further using in sesame breeding program to resistance to P. interpunctella.GÉSSICA LAIZE BERTO GOMES, UEPB; ROSEANE CAVALCANTI DOS SANTOS, CNPA; FABIO AQUINO DE ALBUQUERQUE, CNPA; NAIR HELENA CASTRO ARRIEL, CNPA; LIZIANE MARIA DE LIMA, CNPA.GOMES, G. L. B.SANTOS, R. C. dosALBUQUERQUE, F. A. deARRIEL, N. H. C.LIMA, L. M. de2016-03-18T11:11:11Z2016-03-18T11:11:11Z2016-03-1820152016-03-21T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAfrican Journal of Agricultural Research, v. 10. n. 35, p. 3517-3524, Aug. 2015.1991-637Xhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1041438enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T02:47:45Zoai:www.alice.cnptia.embrapa.br:doc/1041438Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T02:47:45Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae (Hübner) (Lepidoptera: Pyralidae). |
title |
Bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae (Hübner) (Lepidoptera: Pyralidae). |
spellingShingle |
Bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae (Hübner) (Lepidoptera: Pyralidae). GOMES, G. L. B. Sesamum indicum L Tripisina Seed Plodia interpunctella Gergelim Semente Genótipo Lepidoptera |
title_short |
Bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae (Hübner) (Lepidoptera: Pyralidae). |
title_full |
Bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae (Hübner) (Lepidoptera: Pyralidae). |
title_fullStr |
Bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae (Hübner) (Lepidoptera: Pyralidae). |
title_full_unstemmed |
Bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae (Hübner) (Lepidoptera: Pyralidae). |
title_sort |
Bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae (Hübner) (Lepidoptera: Pyralidae). |
author |
GOMES, G. L. B. |
author_facet |
GOMES, G. L. B. SANTOS, R. C. dos ALBUQUERQUE, F. A. de ARRIEL, N. H. C. LIMA, L. M. de |
author_role |
author |
author2 |
SANTOS, R. C. dos ALBUQUERQUE, F. A. de ARRIEL, N. H. C. LIMA, L. M. de |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
GÉSSICA LAIZE BERTO GOMES, UEPB; ROSEANE CAVALCANTI DOS SANTOS, CNPA; FABIO AQUINO DE ALBUQUERQUE, CNPA; NAIR HELENA CASTRO ARRIEL, CNPA; LIZIANE MARIA DE LIMA, CNPA. |
dc.contributor.author.fl_str_mv |
GOMES, G. L. B. SANTOS, R. C. dos ALBUQUERQUE, F. A. de ARRIEL, N. H. C. LIMA, L. M. de |
dc.subject.por.fl_str_mv |
Sesamum indicum L Tripisina Seed Plodia interpunctella Gergelim Semente Genótipo Lepidoptera |
topic |
Sesamum indicum L Tripisina Seed Plodia interpunctella Gergelim Semente Genótipo Lepidoptera |
description |
Biochemical and feeding assays were performed in order to assess the bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae. The biochemical assays were based on the ability of protease inhibitors (PI) present in sesame seeds to inhibit bovine pancreatic trypsin and digestive enzymes of insect larvae. The stability of the inhibitors was also assessed at different pH and temperature. The feeding bioassays were carried out in plates containing second instar larvae fed on diet with sesame protein extract. It was observed that all genotypes presented inhibition when tested with BPT, ranging from 51 to 90%, among them BRS Seda, CNPA G3 and CNPA G4 showed average of 69% of inhibitory activity for intestinal homogenate. The FII-fractions in these three genotypes showed thermal and pH stabilities at 40°C and 8.5, respectively. In feeding bioassay, P. interpunctella larvae were susceptible to SPCE of all three genotypes, revealing average mortality of 74% to SPCE and 45% to FII-fractions. Among all genotypes, BRS Seda was more effective to inhibition of digestive enzymes of insects and considered the more suitable genotype for further using in sesame breeding program to resistance to P. interpunctella. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2016-03-18T11:11:11Z 2016-03-18T11:11:11Z 2016-03-18 2016-03-21T11:11:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
African Journal of Agricultural Research, v. 10. n. 35, p. 3517-3524, Aug. 2015. 1991-637X http://www.alice.cnptia.embrapa.br/alice/handle/doc/1041438 |
identifier_str_mv |
African Journal of Agricultural Research, v. 10. n. 35, p. 3517-3524, Aug. 2015. 1991-637X |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1041438 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1822721192311652352 |