Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea.
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120225 |
Resumo: | A new bipartite begomovirus (family Geminiviridae) was detected on cowpea (Vigna unguiculata) plants exhibiting bright golden mosaic symptoms on leaves under field conditions in Brazil. Complete consensus sequences of DNA-A and DNA-B components of an isolate of the virus (PE?088) were obtained by nanopore sequencing and confirmed by Sanger sequencing. The genome components presented the typical genomic organization of New World (NW) begomoviruses. Pairwise sequence comparisons revealed low levels of identity with other begomovirus species previously reported infecting cowpea around the world. Phylogenetic analysis using complete sequences of DNA-A components revealed that the closest relatives of PE?088 (85-87% nucleotide sequence identities) were three legume-infecting begomoviruses from Brazil: bean golden mosaic virus, macroptilium common mosaic virus and macroptilium yellow vein virus. According to the current classification criteria, PE?088 represents a new species in the genus Begomovirus, tentatively named as cowpea bright yellow mosaic virus (CoBYMV). |
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Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea.Feijao caupiNanoporeCaupiMosca BrancaFeijãoDoençaGeminiviridaeVigna unguiculata subsp. unguiculata var. spontaneaBean golden mosaic virusBegomovirusA new bipartite begomovirus (family Geminiviridae) was detected on cowpea (Vigna unguiculata) plants exhibiting bright golden mosaic symptoms on leaves under field conditions in Brazil. Complete consensus sequences of DNA-A and DNA-B components of an isolate of the virus (PE?088) were obtained by nanopore sequencing and confirmed by Sanger sequencing. The genome components presented the typical genomic organization of New World (NW) begomoviruses. Pairwise sequence comparisons revealed low levels of identity with other begomovirus species previously reported infecting cowpea around the world. Phylogenetic analysis using complete sequences of DNA-A components revealed that the closest relatives of PE?088 (85-87% nucleotide sequence identities) were three legume-infecting begomoviruses from Brazil: bean golden mosaic virus, macroptilium common mosaic virus and macroptilium yellow vein virus. According to the current classification criteria, PE?088 represents a new species in the genus Begomovirus, tentatively named as cowpea bright yellow mosaic virus (CoBYMV).FERNANDA Y. B. NAITO, UNIVERSIDADE DE BRASÍLIA; FERNANDO L. MELO, UNIVERSIDADE DE BRASÍLIA; MARIA ESTHER DE N FONSECA BOITEUX, CNPH; CARLOS ANTONIO FERNANDES SANTOS, CPATSA; CAROLINA R. CHANES, UNIVERSIDADE CATÓLICA DE BRASÍLIA; BERGMANN M. RIBEIRO, UNIVERSIDADE DE BRASÍLIA; ROBERT L. GILBERTSON, UNIVERSITY OF CALIFORNIA; LEONARDO SILVA BOITEUX, CNPH; RITA DE CÁSSIA PEREIRA-CARVALHO, UNIVERSIDADE DE BRASÍLIA.NAITO, F. Y. B.MELO, F. L.FONSECA, M. E. N.SANTOS, C. A. F.CHANES, C. R.RIBEIRO, B. M.GILBERTSON, R. L.BOITEUX, L. S.PEREIRA-CARVALHO, R. de C.2020-02-13T18:16:37Z2020-02-13T18:16:37Z2020-02-1320192020-02-13T18:16:37Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleArchives of Virology, v. 164, n. 7, p. 1907-1910, 2019.http://www.alice.cnptia.embrapa.br/alice/handle/doc/112022510.1007/s00705-019-04254-5enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-02-13T18:16:43Zoai:www.alice.cnptia.embrapa.br:doc/1120225Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-02-13T18:16:43falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-02-13T18:16:43Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea. |
title |
Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea. |
spellingShingle |
Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea. NAITO, F. Y. B. Feijao caupi Nanopore Caupi Mosca Branca Feijão Doença Geminiviridae Vigna unguiculata subsp. unguiculata var. spontanea Bean golden mosaic virus Begomovirus |
title_short |
Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea. |
title_full |
Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea. |
title_fullStr |
Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea. |
title_full_unstemmed |
Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea. |
title_sort |
Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea. |
author |
NAITO, F. Y. B. |
author_facet |
NAITO, F. Y. B. MELO, F. L. FONSECA, M. E. N. SANTOS, C. A. F. CHANES, C. R. RIBEIRO, B. M. GILBERTSON, R. L. BOITEUX, L. S. PEREIRA-CARVALHO, R. de C. |
author_role |
author |
author2 |
MELO, F. L. FONSECA, M. E. N. SANTOS, C. A. F. CHANES, C. R. RIBEIRO, B. M. GILBERTSON, R. L. BOITEUX, L. S. PEREIRA-CARVALHO, R. de C. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
FERNANDA Y. B. NAITO, UNIVERSIDADE DE BRASÍLIA; FERNANDO L. MELO, UNIVERSIDADE DE BRASÍLIA; MARIA ESTHER DE N FONSECA BOITEUX, CNPH; CARLOS ANTONIO FERNANDES SANTOS, CPATSA; CAROLINA R. CHANES, UNIVERSIDADE CATÓLICA DE BRASÍLIA; BERGMANN M. RIBEIRO, UNIVERSIDADE DE BRASÍLIA; ROBERT L. GILBERTSON, UNIVERSITY OF CALIFORNIA; LEONARDO SILVA BOITEUX, CNPH; RITA DE CÁSSIA PEREIRA-CARVALHO, UNIVERSIDADE DE BRASÍLIA. |
dc.contributor.author.fl_str_mv |
NAITO, F. Y. B. MELO, F. L. FONSECA, M. E. N. SANTOS, C. A. F. CHANES, C. R. RIBEIRO, B. M. GILBERTSON, R. L. BOITEUX, L. S. PEREIRA-CARVALHO, R. de C. |
dc.subject.por.fl_str_mv |
Feijao caupi Nanopore Caupi Mosca Branca Feijão Doença Geminiviridae Vigna unguiculata subsp. unguiculata var. spontanea Bean golden mosaic virus Begomovirus |
topic |
Feijao caupi Nanopore Caupi Mosca Branca Feijão Doença Geminiviridae Vigna unguiculata subsp. unguiculata var. spontanea Bean golden mosaic virus Begomovirus |
description |
A new bipartite begomovirus (family Geminiviridae) was detected on cowpea (Vigna unguiculata) plants exhibiting bright golden mosaic symptoms on leaves under field conditions in Brazil. Complete consensus sequences of DNA-A and DNA-B components of an isolate of the virus (PE?088) were obtained by nanopore sequencing and confirmed by Sanger sequencing. The genome components presented the typical genomic organization of New World (NW) begomoviruses. Pairwise sequence comparisons revealed low levels of identity with other begomovirus species previously reported infecting cowpea around the world. Phylogenetic analysis using complete sequences of DNA-A components revealed that the closest relatives of PE?088 (85-87% nucleotide sequence identities) were three legume-infecting begomoviruses from Brazil: bean golden mosaic virus, macroptilium common mosaic virus and macroptilium yellow vein virus. According to the current classification criteria, PE?088 represents a new species in the genus Begomovirus, tentatively named as cowpea bright yellow mosaic virus (CoBYMV). |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2020-02-13T18:16:37Z 2020-02-13T18:16:37Z 2020-02-13 2020-02-13T18:16:37Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Archives of Virology, v. 164, n. 7, p. 1907-1910, 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120225 10.1007/s00705-019-04254-5 |
identifier_str_mv |
Archives of Virology, v. 164, n. 7, p. 1907-1910, 2019. 10.1007/s00705-019-04254-5 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120225 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
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EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503490083487744 |