Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows.
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/902309 https://doi.org/10.4238/vol10-3gmr1096 |
Resumo: | Among the potential public health problems of animal production, infectious-contagious diseases stand out. Mastitis is among the main diseases affecting dairy cattle. One of the most promising options to reduce the problems caused by this disease, besides proper sanitary and management practices, is selective breeding of resistant animals. To shed light on the immune response mechanisms involved in the resistance/susceptibility phenotype to this disease, we quantified the relative expression of the genes IL-2, IL-6, IL-8, IL-12, IFN-γ, TNF-α, TLR-2, SEMA5A, and FEZL in cells of crossbreed dairy cows, divided into two groups, one healthy and the other suffering from clinical mastitis. Total RNA was extracted from the cells in the milk from the animals in each group (with and without clinical mastitis). Gene expression was determined using the real-time PCR method. The levels of gene expression were compared, and the cows with mastitis were found to express 2.5 times more TLR-2 than those free of mastitis (P < 0.05). There were no significant differences in the expression of the other genes. |
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Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows.Real-time PCRResistance to mastitisToll-like receptor 2 geneimmune responseAmong the potential public health problems of animal production, infectious-contagious diseases stand out. Mastitis is among the main diseases affecting dairy cattle. One of the most promising options to reduce the problems caused by this disease, besides proper sanitary and management practices, is selective breeding of resistant animals. To shed light on the immune response mechanisms involved in the resistance/susceptibility phenotype to this disease, we quantified the relative expression of the genes IL-2, IL-6, IL-8, IL-12, IFN-γ, TNF-α, TLR-2, SEMA5A, and FEZL in cells of crossbreed dairy cows, divided into two groups, one healthy and the other suffering from clinical mastitis. Total RNA was extracted from the cells in the milk from the animals in each group (with and without clinical mastitis). Gene expression was determined using the real-time PCR method. The levels of gene expression were compared, and the cows with mastitis were found to express 2.5 times more TLR-2 than those free of mastitis (P < 0.05). There were no significant differences in the expression of the other genes.ISABELA FONSECA, UFV; GUSTAVO RESENDE ANTUNES, UFJF; DAISYLÉA DE SOUZA PAIVA, UFJF; CARLA CHRISTINE LANGE, CNPGL; SIMONE ELISA FACIONI GUIMARÃES, UFV; MARTA FONSECA MARTINS, CNPGL.FONSECA, I.ANTUNES, G. R.PAIVA, D. de S.LANGE, C. C.GUIMARÃES, S. E. F.MARTINS, M. F.2024-02-06T13:33:09Z2024-02-06T13:33:09Z2011-10-042011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 10, n. 3, p. 1295-1303, 2011.http://www.alice.cnptia.embrapa.br/alice/handle/doc/902309https://doi.org/10.4238/vol10-3gmr1096enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2024-02-06T13:33:09Zoai:www.alice.cnptia.embrapa.br:doc/902309Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542024-02-06T13:33:09falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542024-02-06T13:33:09Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows. |
title |
Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows. |
spellingShingle |
Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows. FONSECA, I. Real-time PCR Resistance to mastitis Toll-like receptor 2 gene immune response |
title_short |
Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows. |
title_full |
Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows. |
title_fullStr |
Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows. |
title_full_unstemmed |
Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows. |
title_sort |
Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows. |
author |
FONSECA, I. |
author_facet |
FONSECA, I. ANTUNES, G. R. PAIVA, D. de S. LANGE, C. C. GUIMARÃES, S. E. F. MARTINS, M. F. |
author_role |
author |
author2 |
ANTUNES, G. R. PAIVA, D. de S. LANGE, C. C. GUIMARÃES, S. E. F. MARTINS, M. F. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
ISABELA FONSECA, UFV; GUSTAVO RESENDE ANTUNES, UFJF; DAISYLÉA DE SOUZA PAIVA, UFJF; CARLA CHRISTINE LANGE, CNPGL; SIMONE ELISA FACIONI GUIMARÃES, UFV; MARTA FONSECA MARTINS, CNPGL. |
dc.contributor.author.fl_str_mv |
FONSECA, I. ANTUNES, G. R. PAIVA, D. de S. LANGE, C. C. GUIMARÃES, S. E. F. MARTINS, M. F. |
dc.subject.por.fl_str_mv |
Real-time PCR Resistance to mastitis Toll-like receptor 2 gene immune response |
topic |
Real-time PCR Resistance to mastitis Toll-like receptor 2 gene immune response |
description |
Among the potential public health problems of animal production, infectious-contagious diseases stand out. Mastitis is among the main diseases affecting dairy cattle. One of the most promising options to reduce the problems caused by this disease, besides proper sanitary and management practices, is selective breeding of resistant animals. To shed light on the immune response mechanisms involved in the resistance/susceptibility phenotype to this disease, we quantified the relative expression of the genes IL-2, IL-6, IL-8, IL-12, IFN-γ, TNF-α, TLR-2, SEMA5A, and FEZL in cells of crossbreed dairy cows, divided into two groups, one healthy and the other suffering from clinical mastitis. Total RNA was extracted from the cells in the milk from the animals in each group (with and without clinical mastitis). Gene expression was determined using the real-time PCR method. The levels of gene expression were compared, and the cows with mastitis were found to express 2.5 times more TLR-2 than those free of mastitis (P < 0.05). There were no significant differences in the expression of the other genes. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-10-04 2011 2024-02-06T13:33:09Z 2024-02-06T13:33:09Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, v. 10, n. 3, p. 1295-1303, 2011. http://www.alice.cnptia.embrapa.br/alice/handle/doc/902309 https://doi.org/10.4238/vol10-3gmr1096 |
identifier_str_mv |
Genetics and Molecular Research, v. 10, n. 3, p. 1295-1303, 2011. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/902309 https://doi.org/10.4238/vol10-3gmr1096 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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