Spinning gland transcriptomics from two main Clades of Spiders (Order: Araneae) - insights on their molecular, anatomical and behavioral evolution.
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/920286 |
Resumo: | Characterized by distinctive evolutionary adaptations, spiders provide a comprehensive system for evolutionary and developmental studies of anatomical organs, including silk and venom production. Here we performed cDNA sequencing using massively parallel sequencers (454 GS-FLX Titanium) to generate ~80,000 reads from the spinning gland of Actinopus spp. (infraorder: Mygalomorphae) and Gasteracantha cancriformis (infraorder: Araneomorphae, Orbiculariae clade). Actinopus spp. retains primitive characteristics on web usage and presents a single undifferentiated spinning gland while the orbiculariae spiders have seven differentiated spinning glands and complex patterns of web usage. MIRA, Celera Assembler and CAP3 software were used to cluster NGS reads for each spider. CAP3 unigenes passed through a pipeline for automatic annotation, classification by biological function, and comparative transcriptomics. Genes related to spider silks were manually curated and analyzed. Although a single spidroin gene family was found in Actinopus spp., a vast repertoire of specialized spider silk proteins was encountered in orbiculariae. Astacin-like metalloproteases (meprin subfamily) were shown to be some of the most sampled unigenes and duplicated gene families in G. cancriformis since its evolutionary split from mygalomorphs. Our results confirm that the evolution of the molecular repertoire of silk proteins was accompanied by the (i) anatomical differentiation of spinning glands and (ii) behavioral complexification in the web usage. Finally, a phylogenetic tree was constructed to cluster most of the known spidroins in gene clades. This is the first large-scale, multi-organism transcriptome for spider spinning glands and a first step into a broad understanding of spider web systems biology and evolution. |
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Spinning gland transcriptomics from two main Clades of Spiders (Order: Araneae) - insights on their molecular, anatomical and behavioral evolution.Sequencia de DNASequenciamentoAranhasCharacterized by distinctive evolutionary adaptations, spiders provide a comprehensive system for evolutionary and developmental studies of anatomical organs, including silk and venom production. Here we performed cDNA sequencing using massively parallel sequencers (454 GS-FLX Titanium) to generate ~80,000 reads from the spinning gland of Actinopus spp. (infraorder: Mygalomorphae) and Gasteracantha cancriformis (infraorder: Araneomorphae, Orbiculariae clade). Actinopus spp. retains primitive characteristics on web usage and presents a single undifferentiated spinning gland while the orbiculariae spiders have seven differentiated spinning glands and complex patterns of web usage. MIRA, Celera Assembler and CAP3 software were used to cluster NGS reads for each spider. CAP3 unigenes passed through a pipeline for automatic annotation, classification by biological function, and comparative transcriptomics. Genes related to spider silks were manually curated and analyzed. Although a single spidroin gene family was found in Actinopus spp., a vast repertoire of specialized spider silk proteins was encountered in orbiculariae. Astacin-like metalloproteases (meprin subfamily) were shown to be some of the most sampled unigenes and duplicated gene families in G. cancriformis since its evolutionary split from mygalomorphs. Our results confirm that the evolution of the molecular repertoire of silk proteins was accompanied by the (i) anatomical differentiation of spinning glands and (ii) behavioral complexification in the web usage. Finally, a phylogenetic tree was constructed to cluster most of the known spidroins in gene clades. This is the first large-scale, multi-organism transcriptome for spider spinning glands and a first step into a broad understanding of spider web systems biology and evolution.FRANCISCO PROSDOCIMI, UFRJ, UCB; DANIELA MATIAS DE C BITTENCOURT, CPAA; FELIPE RODRIGUES DA SILVA, CNPTIA; MATIAS KIRST, University of Florida; PAULO C. MOTA, UnB; ELIBIO LEOPOLDO RECH FILHO, CENARGEN.PROSDOCIMI, PBITTENCOURT, D.SILVA, F. R. daKIRST, M.MOTA, P. C.RECH, E. L.2018-02-05T23:32:25Z2018-02-05T23:32:25Z2012-03-2620112018-02-05T23:32:25Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlos One, San Francisco, v. 6, n. 6, p. 1-15, June 2011.http://www.alice.cnptia.embrapa.br/alice/handle/doc/92028610.1371/journal.pone.0021634enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-02-05T23:32:32Zoai:www.alice.cnptia.embrapa.br:doc/920286Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-02-05T23:32:32falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-02-05T23:32:32Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Spinning gland transcriptomics from two main Clades of Spiders (Order: Araneae) - insights on their molecular, anatomical and behavioral evolution. |
title |
Spinning gland transcriptomics from two main Clades of Spiders (Order: Araneae) - insights on their molecular, anatomical and behavioral evolution. |
spellingShingle |
Spinning gland transcriptomics from two main Clades of Spiders (Order: Araneae) - insights on their molecular, anatomical and behavioral evolution. PROSDOCIMI, P Sequencia de DNA Sequenciamento Aranhas |
title_short |
Spinning gland transcriptomics from two main Clades of Spiders (Order: Araneae) - insights on their molecular, anatomical and behavioral evolution. |
title_full |
Spinning gland transcriptomics from two main Clades of Spiders (Order: Araneae) - insights on their molecular, anatomical and behavioral evolution. |
title_fullStr |
Spinning gland transcriptomics from two main Clades of Spiders (Order: Araneae) - insights on their molecular, anatomical and behavioral evolution. |
title_full_unstemmed |
Spinning gland transcriptomics from two main Clades of Spiders (Order: Araneae) - insights on their molecular, anatomical and behavioral evolution. |
title_sort |
Spinning gland transcriptomics from two main Clades of Spiders (Order: Araneae) - insights on their molecular, anatomical and behavioral evolution. |
author |
PROSDOCIMI, P |
author_facet |
PROSDOCIMI, P BITTENCOURT, D. SILVA, F. R. da KIRST, M. MOTA, P. C. RECH, E. L. |
author_role |
author |
author2 |
BITTENCOURT, D. SILVA, F. R. da KIRST, M. MOTA, P. C. RECH, E. L. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
FRANCISCO PROSDOCIMI, UFRJ, UCB; DANIELA MATIAS DE C BITTENCOURT, CPAA; FELIPE RODRIGUES DA SILVA, CNPTIA; MATIAS KIRST, University of Florida; PAULO C. MOTA, UnB; ELIBIO LEOPOLDO RECH FILHO, CENARGEN. |
dc.contributor.author.fl_str_mv |
PROSDOCIMI, P BITTENCOURT, D. SILVA, F. R. da KIRST, M. MOTA, P. C. RECH, E. L. |
dc.subject.por.fl_str_mv |
Sequencia de DNA Sequenciamento Aranhas |
topic |
Sequencia de DNA Sequenciamento Aranhas |
description |
Characterized by distinctive evolutionary adaptations, spiders provide a comprehensive system for evolutionary and developmental studies of anatomical organs, including silk and venom production. Here we performed cDNA sequencing using massively parallel sequencers (454 GS-FLX Titanium) to generate ~80,000 reads from the spinning gland of Actinopus spp. (infraorder: Mygalomorphae) and Gasteracantha cancriformis (infraorder: Araneomorphae, Orbiculariae clade). Actinopus spp. retains primitive characteristics on web usage and presents a single undifferentiated spinning gland while the orbiculariae spiders have seven differentiated spinning glands and complex patterns of web usage. MIRA, Celera Assembler and CAP3 software were used to cluster NGS reads for each spider. CAP3 unigenes passed through a pipeline for automatic annotation, classification by biological function, and comparative transcriptomics. Genes related to spider silks were manually curated and analyzed. Although a single spidroin gene family was found in Actinopus spp., a vast repertoire of specialized spider silk proteins was encountered in orbiculariae. Astacin-like metalloproteases (meprin subfamily) were shown to be some of the most sampled unigenes and duplicated gene families in G. cancriformis since its evolutionary split from mygalomorphs. Our results confirm that the evolution of the molecular repertoire of silk proteins was accompanied by the (i) anatomical differentiation of spinning glands and (ii) behavioral complexification in the web usage. Finally, a phylogenetic tree was constructed to cluster most of the known spidroins in gene clades. This is the first large-scale, multi-organism transcriptome for spider spinning glands and a first step into a broad understanding of spider web systems biology and evolution. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2012-03-26 2018-02-05T23:32:25Z 2018-02-05T23:32:25Z 2018-02-05T23:32:25Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Plos One, San Francisco, v. 6, n. 6, p. 1-15, June 2011. http://www.alice.cnptia.embrapa.br/alice/handle/doc/920286 10.1371/journal.pone.0021634 |
identifier_str_mv |
Plos One, San Francisco, v. 6, n. 6, p. 1-15, June 2011. 10.1371/journal.pone.0021634 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/920286 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503449305415680 |