Genetic analysis of opaque2 modifier gene activity in maize endosperm.
Autor(a) principal: | |
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Data de Publicação: | 1995 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/471684 |
Resumo: | Modifier genes have been described that convert the soft endosperm of opaque2 mutants to a hard, vitreous phenotype. The mode of action and the components of the genetic system involved in this seed modification ar poorly understood. We used genetic and biochemical analyses to investigate the number of opaque2 modifier genes, their mode of action and their relationship to the biochemical alterations in the modified endosperm. Using two inbred opaque2 lines, we showed that the activity of opaque2 modifier genes is influenced by the genetic back-ground. Analysis of segregating progenies and recombinant inbred lines derived from crosses between opaque2 and modified opaque2 genotypes indicated two independent loci affecting seed opacity and density. Consistent association between endosperm modification and enhanced accumulation of the gamma-zein storage protein suggested that either this protein is directly involved in the process of seed modification, or else that a modifier gene could be tightly linked to the genes responsible for gamma-zein synthesis. |
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Genetic analysis of opaque2 modifier gene activity in maize endosperm.Opaco2Analise geneticaGem modificadosMaizeGenetic analysisModifier genesRecombinant inbredsOpaque2ZeinsMilhoZeínaendospermModifier genes have been described that convert the soft endosperm of opaque2 mutants to a hard, vitreous phenotype. The mode of action and the components of the genetic system involved in this seed modification ar poorly understood. We used genetic and biochemical analyses to investigate the number of opaque2 modifier genes, their mode of action and their relationship to the biochemical alterations in the modified endosperm. Using two inbred opaque2 lines, we showed that the activity of opaque2 modifier genes is influenced by the genetic back-ground. Analysis of segregating progenies and recombinant inbred lines derived from crosses between opaque2 and modified opaque2 genotypes indicated two independent loci affecting seed opacity and density. Consistent association between endosperm modification and enhanced accumulation of the gamma-zein storage protein suggested that either this protein is directly involved in the process of seed modification, or else that a modifier gene could be tightly linked to the genes responsible for gamma-zein synthesis.EMBRAPA-CNPMS.LOPES, M. A.LARKINS, B. A.2020-08-24T04:10:14Z2020-08-24T04:10:14Z1996-04-291995info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTheoretical and Applied Genetics, Berlin, v. 91, n. 1, p. 274-281, 1995.http://www.alice.cnptia.embrapa.br/alice/handle/doc/471684enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-08-24T04:10:39Zoai:www.alice.cnptia.embrapa.br:doc/471684Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-08-24T04:10:39falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-08-24T04:10:39Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genetic analysis of opaque2 modifier gene activity in maize endosperm. |
title |
Genetic analysis of opaque2 modifier gene activity in maize endosperm. |
spellingShingle |
Genetic analysis of opaque2 modifier gene activity in maize endosperm. LOPES, M. A. Opaco2 Analise genetica Gem modificados Maize Genetic analysis Modifier genes Recombinant inbreds Opaque2 Zeins Milho Zeína endosperm |
title_short |
Genetic analysis of opaque2 modifier gene activity in maize endosperm. |
title_full |
Genetic analysis of opaque2 modifier gene activity in maize endosperm. |
title_fullStr |
Genetic analysis of opaque2 modifier gene activity in maize endosperm. |
title_full_unstemmed |
Genetic analysis of opaque2 modifier gene activity in maize endosperm. |
title_sort |
Genetic analysis of opaque2 modifier gene activity in maize endosperm. |
author |
LOPES, M. A. |
author_facet |
LOPES, M. A. LARKINS, B. A. |
author_role |
author |
author2 |
LARKINS, B. A. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
EMBRAPA-CNPMS. |
dc.contributor.author.fl_str_mv |
LOPES, M. A. LARKINS, B. A. |
dc.subject.por.fl_str_mv |
Opaco2 Analise genetica Gem modificados Maize Genetic analysis Modifier genes Recombinant inbreds Opaque2 Zeins Milho Zeína endosperm |
topic |
Opaco2 Analise genetica Gem modificados Maize Genetic analysis Modifier genes Recombinant inbreds Opaque2 Zeins Milho Zeína endosperm |
description |
Modifier genes have been described that convert the soft endosperm of opaque2 mutants to a hard, vitreous phenotype. The mode of action and the components of the genetic system involved in this seed modification ar poorly understood. We used genetic and biochemical analyses to investigate the number of opaque2 modifier genes, their mode of action and their relationship to the biochemical alterations in the modified endosperm. Using two inbred opaque2 lines, we showed that the activity of opaque2 modifier genes is influenced by the genetic back-ground. Analysis of segregating progenies and recombinant inbred lines derived from crosses between opaque2 and modified opaque2 genotypes indicated two independent loci affecting seed opacity and density. Consistent association between endosperm modification and enhanced accumulation of the gamma-zein storage protein suggested that either this protein is directly involved in the process of seed modification, or else that a modifier gene could be tightly linked to the genes responsible for gamma-zein synthesis. |
publishDate |
1995 |
dc.date.none.fl_str_mv |
1995 1996-04-29 2020-08-24T04:10:14Z 2020-08-24T04:10:14Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Theoretical and Applied Genetics, Berlin, v. 91, n. 1, p. 274-281, 1995. http://www.alice.cnptia.embrapa.br/alice/handle/doc/471684 |
identifier_str_mv |
Theoretical and Applied Genetics, Berlin, v. 91, n. 1, p. 274-281, 1995. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/471684 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503494868140032 |