New high-sensitive rhAmp method for A1 allele detection in A2 milk samples.
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121088 https://doi.org/10.1016/j.foodchem.2020.126167 |
Resumo: | Cows milk may contain two types of - casein: A1 and A2. A1 digestion is associated with the release of - casomorphine-7 peptide, which can cause adverse gastrointestinal effects. Two methods high-resolution melting (HRM) and rhAmp® SNP genotyping - were developed to identify the - casein gene (CSN2) A1 and A2 alleles directly in milk. DNA milk samples from 45 animals were examined and 10 samples were also sequenced to confirm the accuracy of the assays. The analytical sensitivities of both strategies for A1 allele identification were evaluated by testing decreasing dilutions of A1 allele DNA copies (500 - 5 copies) in the A2 sample. The limits of detection for A1 in A2 samples were 10% (100 copies) and 2% (10 copies) for HRM and rhAmp, respectively. Both techniques were specific, differentiating between A1 and A2 alleles. However, we recommend rhAmp genotyping testing over HRM because of its enhanced sensitivity for A1. |
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New high-sensitive rhAmp method for A1 allele detection in A2 milk samples.B caseinHRMRhAmpGenotypingAllelesCows milk may contain two types of - casein: A1 and A2. A1 digestion is associated with the release of - casomorphine-7 peptide, which can cause adverse gastrointestinal effects. Two methods high-resolution melting (HRM) and rhAmp® SNP genotyping - were developed to identify the - casein gene (CSN2) A1 and A2 alleles directly in milk. DNA milk samples from 45 animals were examined and 10 samples were also sequenced to confirm the accuracy of the assays. The analytical sensitivities of both strategies for A1 allele identification were evaluated by testing decreasing dilutions of A1 allele DNA copies (500 - 5 copies) in the A2 sample. The limits of detection for A1 in A2 samples were 10% (100 copies) and 2% (10 copies) for HRM and rhAmp, respectively. Both techniques were specific, differentiating between A1 and A2 alleles. However, we recommend rhAmp genotyping testing over HRM because of its enhanced sensitivity for A1.Rodrigo Giglioti, Instituto de Zootecnia; Gunta Gutmanis, Instituto de Zootecnia; Luciana Morita Katiki, Instituto de Zootecnia; CINTIA HIROMI OKINO, CPPSE; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; Anibal Eugênio Vercesi Filho, Instituto de Zootecnia.GIGLIOTI, R.GUTMANIS, G.KATIKI, L. M.OKINO, C. H.OLIVEIRA, M. C. de S.VERCESI FILHO, A. E.2020-04-03T00:39:33Z2020-04-03T00:39:33Z2020-03-1020202020-04-20T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7 p.Food Chemistry, v. 313, 2020. 126167.0308-8146http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121088https://doi.org/10.1016/j.foodchem.2020.126167enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-04-03T00:39:40Zoai:www.alice.cnptia.embrapa.br:doc/1121088Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-04-03T00:39:40falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-04-03T00:39:40Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
New high-sensitive rhAmp method for A1 allele detection in A2 milk samples. |
title |
New high-sensitive rhAmp method for A1 allele detection in A2 milk samples. |
spellingShingle |
New high-sensitive rhAmp method for A1 allele detection in A2 milk samples. GIGLIOTI, R. B casein HRM RhAmp Genotyping Alleles |
title_short |
New high-sensitive rhAmp method for A1 allele detection in A2 milk samples. |
title_full |
New high-sensitive rhAmp method for A1 allele detection in A2 milk samples. |
title_fullStr |
New high-sensitive rhAmp method for A1 allele detection in A2 milk samples. |
title_full_unstemmed |
New high-sensitive rhAmp method for A1 allele detection in A2 milk samples. |
title_sort |
New high-sensitive rhAmp method for A1 allele detection in A2 milk samples. |
author |
GIGLIOTI, R. |
author_facet |
GIGLIOTI, R. GUTMANIS, G. KATIKI, L. M. OKINO, C. H. OLIVEIRA, M. C. de S. VERCESI FILHO, A. E. |
author_role |
author |
author2 |
GUTMANIS, G. KATIKI, L. M. OKINO, C. H. OLIVEIRA, M. C. de S. VERCESI FILHO, A. E. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Rodrigo Giglioti, Instituto de Zootecnia; Gunta Gutmanis, Instituto de Zootecnia; Luciana Morita Katiki, Instituto de Zootecnia; CINTIA HIROMI OKINO, CPPSE; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; Anibal Eugênio Vercesi Filho, Instituto de Zootecnia. |
dc.contributor.author.fl_str_mv |
GIGLIOTI, R. GUTMANIS, G. KATIKI, L. M. OKINO, C. H. OLIVEIRA, M. C. de S. VERCESI FILHO, A. E. |
dc.subject.por.fl_str_mv |
B casein HRM RhAmp Genotyping Alleles |
topic |
B casein HRM RhAmp Genotyping Alleles |
description |
Cows milk may contain two types of - casein: A1 and A2. A1 digestion is associated with the release of - casomorphine-7 peptide, which can cause adverse gastrointestinal effects. Two methods high-resolution melting (HRM) and rhAmp® SNP genotyping - were developed to identify the - casein gene (CSN2) A1 and A2 alleles directly in milk. DNA milk samples from 45 animals were examined and 10 samples were also sequenced to confirm the accuracy of the assays. The analytical sensitivities of both strategies for A1 allele identification were evaluated by testing decreasing dilutions of A1 allele DNA copies (500 - 5 copies) in the A2 sample. The limits of detection for A1 in A2 samples were 10% (100 copies) and 2% (10 copies) for HRM and rhAmp, respectively. Both techniques were specific, differentiating between A1 and A2 alleles. However, we recommend rhAmp genotyping testing over HRM because of its enhanced sensitivity for A1. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04-03T00:39:33Z 2020-04-03T00:39:33Z 2020-03-10 2020 2020-04-20T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Food Chemistry, v. 313, 2020. 126167. 0308-8146 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121088 https://doi.org/10.1016/j.foodchem.2020.126167 |
identifier_str_mv |
Food Chemistry, v. 313, 2020. 126167. 0308-8146 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121088 https://doi.org/10.1016/j.foodchem.2020.126167 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 p. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503491570368512 |