Pre-Slaughter stress affects ryanodine receptor protein gene expression and the water-holding capacity in fillets of the nile Tilapia.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1026757 |
Resumo: | Current study evaluated the effect of pre-slaughter stress on serum cortisol levels, pH, colorimetry, water-holding capacity (WHC) and gene expression of ryanodine receptors (RyR1 and RyR3) in the Nile tilapia. A 3x4 factorial scheme experiment was conducted comprising three densities (100, 200, 400 kg/m³) with four transportation times (60, 120, 180, and 240 minutes).Transportation times alone reduced cortisol levels up to 180 minutes, followed by increased WHC and mRNA expression, RyR1 and RyR3 (200 kg/m³ density). No effect of density x transportation time interacted on the evaluated parameters. Results provided the first evidence that pre-slaughter stress affected ryanodine gene expression receptors and, consequently, the water-holding capacity in tilapia fillets. |
id |
EMBR_bb8fe25039fbc3d98998167b848f6493 |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/1026757 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
Pre-Slaughter stress affects ryanodine receptor protein gene expression and the water-holding capacity in fillets of the nile Tilapia.Live fishFish behaviourTilapiaPeixeStressFish meatSlaughterfishCurrent study evaluated the effect of pre-slaughter stress on serum cortisol levels, pH, colorimetry, water-holding capacity (WHC) and gene expression of ryanodine receptors (RyR1 and RyR3) in the Nile tilapia. A 3x4 factorial scheme experiment was conducted comprising three densities (100, 200, 400 kg/m³) with four transportation times (60, 120, 180, and 240 minutes).Transportation times alone reduced cortisol levels up to 180 minutes, followed by increased WHC and mRNA expression, RyR1 and RyR3 (200 kg/m³ density). No effect of density x transportation time interacted on the evaluated parameters. Results provided the first evidence that pre-slaughter stress affected ryanodine gene expression receptors and, consequently, the water-holding capacity in tilapia fillets.ELENICE S. R. GOES, UEM; JORGE ANTONIO FERREIRA DE LARA, CPAP; ELIANE GASPARINO, UEM; ANA P. DEL VESCO, UEM; MARCIO D. GOES, UFPR; LUIZ ALEXANDRE FILHO, UEM; RICARDO P. RIBEIRO, UEM.GOES, E. S. R.LARA, J. A. F. deGASPARINO, E.DEL VESCO, A. P.GOES, M. D.ALEXANDRE FILHO, L.RIBEIRO, R. P.2015-10-20T11:11:11Z2015-10-20T11:11:11Z2015-10-2020152016-01-28T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlos One, v. 10, n. 6, p. 1-14, junho, 2015.http://www.alice.cnptia.embrapa.br/alice/handle/doc/102675710.1371/journal.pone.0129145enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T02:56:24Zoai:www.alice.cnptia.embrapa.br:doc/1026757Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T02:56:24falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T02:56:24Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Pre-Slaughter stress affects ryanodine receptor protein gene expression and the water-holding capacity in fillets of the nile Tilapia. |
title |
Pre-Slaughter stress affects ryanodine receptor protein gene expression and the water-holding capacity in fillets of the nile Tilapia. |
spellingShingle |
Pre-Slaughter stress affects ryanodine receptor protein gene expression and the water-holding capacity in fillets of the nile Tilapia. GOES, E. S. R. Live fish Fish behaviour Tilapia Peixe Stress Fish meat Slaughter fish |
title_short |
Pre-Slaughter stress affects ryanodine receptor protein gene expression and the water-holding capacity in fillets of the nile Tilapia. |
title_full |
Pre-Slaughter stress affects ryanodine receptor protein gene expression and the water-holding capacity in fillets of the nile Tilapia. |
title_fullStr |
Pre-Slaughter stress affects ryanodine receptor protein gene expression and the water-holding capacity in fillets of the nile Tilapia. |
title_full_unstemmed |
Pre-Slaughter stress affects ryanodine receptor protein gene expression and the water-holding capacity in fillets of the nile Tilapia. |
title_sort |
Pre-Slaughter stress affects ryanodine receptor protein gene expression and the water-holding capacity in fillets of the nile Tilapia. |
author |
GOES, E. S. R. |
author_facet |
GOES, E. S. R. LARA, J. A. F. de GASPARINO, E. DEL VESCO, A. P. GOES, M. D. ALEXANDRE FILHO, L. RIBEIRO, R. P. |
author_role |
author |
author2 |
LARA, J. A. F. de GASPARINO, E. DEL VESCO, A. P. GOES, M. D. ALEXANDRE FILHO, L. RIBEIRO, R. P. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
ELENICE S. R. GOES, UEM; JORGE ANTONIO FERREIRA DE LARA, CPAP; ELIANE GASPARINO, UEM; ANA P. DEL VESCO, UEM; MARCIO D. GOES, UFPR; LUIZ ALEXANDRE FILHO, UEM; RICARDO P. RIBEIRO, UEM. |
dc.contributor.author.fl_str_mv |
GOES, E. S. R. LARA, J. A. F. de GASPARINO, E. DEL VESCO, A. P. GOES, M. D. ALEXANDRE FILHO, L. RIBEIRO, R. P. |
dc.subject.por.fl_str_mv |
Live fish Fish behaviour Tilapia Peixe Stress Fish meat Slaughter fish |
topic |
Live fish Fish behaviour Tilapia Peixe Stress Fish meat Slaughter fish |
description |
Current study evaluated the effect of pre-slaughter stress on serum cortisol levels, pH, colorimetry, water-holding capacity (WHC) and gene expression of ryanodine receptors (RyR1 and RyR3) in the Nile tilapia. A 3x4 factorial scheme experiment was conducted comprising three densities (100, 200, 400 kg/m³) with four transportation times (60, 120, 180, and 240 minutes).Transportation times alone reduced cortisol levels up to 180 minutes, followed by increased WHC and mRNA expression, RyR1 and RyR3 (200 kg/m³ density). No effect of density x transportation time interacted on the evaluated parameters. Results provided the first evidence that pre-slaughter stress affected ryanodine gene expression receptors and, consequently, the water-holding capacity in tilapia fillets. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-20T11:11:11Z 2015-10-20T11:11:11Z 2015-10-20 2015 2016-01-28T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Plos One, v. 10, n. 6, p. 1-14, junho, 2015. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1026757 10.1371/journal.pone.0129145 |
identifier_str_mv |
Plos One, v. 10, n. 6, p. 1-14, junho, 2015. 10.1371/journal.pone.0129145 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1026757 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503412801339392 |