Biochemical and phylogenetic characterization of the wastewater tolerant Chlamydomonas biconvexa Embrapa|LBA40 strain cultivated in palm oil mill effluent.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131384 https://doi.org/10.1371/journal.pone.0249089 |
Resumo: | The increasing demand for water, food and energy poses challenges for the world´s sustainability. Tropical palm oil is currently the major source of vegetable oil worldwide with a production that exceeds 55 million tons per year, while generating over 200 million tons of palm oil mill effluent (POME). It could potentially be used as a substrate for production of microalgal biomass though. In this study, the microalgal strain Chlamydomonas biconvexa Embrapa|LBA40, originally isolated from a sugarcane vinasse stabilization pond, was selected among 17 strains tested for growth in POME retrieved from anaerobic ponds of a palm oil industrial plant located within the Amazon rainforest region. During cultivation in POME, C. biconvexa Embrapa|LBA40 biomass productivity reached 190.60 mgDW ? L-1 ? d-1 using 15L airlift flat plate photobioreactors. Carbohydrates comprised the major fraction of algal biomass (31.96%), while the lipidic fraction reached up to 11.3% of dry mass. Reductions of 99% in ammonium and nitrite, as well as 98% reduction in phosphate present in POME were detected after 5 days of algal cultivation. This suggests that the aerobic pond stage, usually used in palm oil industrial plants to reduce POME inorganic load, could be substituted by high rate photobioreactors, significantly reducing the time and area requirements for wastewater treatment. In addition, the complete mitochondrial genome of C. biconvexa Embrapa|LBA40 strain was sequenced, revealing a compact mitogenome, with 15.98 kb in size, a total of 14 genes, of which 9 are protein coding genes. Phylogenetic analysis confirmed the strain taxonomic status within the Chlamydomonas genus, opening up opportunities for future genetic modification and molecular breeding programs in these species. |
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Biochemical and phylogenetic characterization of the wastewater tolerant Chlamydomonas biconvexa Embrapa|LBA40 strain cultivated in palm oil mill effluent.Chlamydomonas biconvexa Embrapa|LBA40BiomassaPalm oilsMicrobial biomassMicrobial geneticsWastewaterThe increasing demand for water, food and energy poses challenges for the world´s sustainability. Tropical palm oil is currently the major source of vegetable oil worldwide with a production that exceeds 55 million tons per year, while generating over 200 million tons of palm oil mill effluent (POME). It could potentially be used as a substrate for production of microalgal biomass though. In this study, the microalgal strain Chlamydomonas biconvexa Embrapa|LBA40, originally isolated from a sugarcane vinasse stabilization pond, was selected among 17 strains tested for growth in POME retrieved from anaerobic ponds of a palm oil industrial plant located within the Amazon rainforest region. During cultivation in POME, C. biconvexa Embrapa|LBA40 biomass productivity reached 190.60 mgDW ? L-1 ? d-1 using 15L airlift flat plate photobioreactors. Carbohydrates comprised the major fraction of algal biomass (31.96%), while the lipidic fraction reached up to 11.3% of dry mass. Reductions of 99% in ammonium and nitrite, as well as 98% reduction in phosphate present in POME were detected after 5 days of algal cultivation. This suggests that the aerobic pond stage, usually used in palm oil industrial plants to reduce POME inorganic load, could be substituted by high rate photobioreactors, significantly reducing the time and area requirements for wastewater treatment. In addition, the complete mitochondrial genome of C. biconvexa Embrapa|LBA40 strain was sequenced, revealing a compact mitogenome, with 15.98 kb in size, a total of 14 genes, of which 9 are protein coding genes. Phylogenetic analysis confirmed the strain taxonomic status within the Chlamydomonas genus, opening up opportunities for future genetic modification and molecular breeding programs in these species.PATRÍCIA VERDUGO PASCOAL, Universidade Federal da BahiaDÁGON MANOEL RIBEIRO, Universidade de BrasíliaCAROLINA RIBEIRO CEREIJOHUGO SANTANARODRIGO CARVALHO NASCIMENTOANDREI STECCA STEINDORFLORENA COSTA GARCIA CALSING, CNPAEEDUARDO FERNANDES FORMIGHIERI, CNPAEBRUNO DOS SANTOS A F BRASIL, SPD.PASCOAL, P. V.RIBEIRO, D. M.CEREIJO, C. R.SANTANA, H.NASCIMENTO, R. C.STEINDORF, A. S.GARCIA, L. C.FORMIGHIERI, E. F.BRASIL, B. dos S. A. F.2021-04-19T19:33:52Z2021-04-19T19:33:52Z2021-04-192021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePLoS One, v. 16, n. 4, e0249089, 2021.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131384https://doi.org/10.1371/journal.pone.0249089enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-04-19T19:34:03Zoai:www.alice.cnptia.embrapa.br:doc/1131384Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-04-19T19:34:03falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-04-19T19:34:03Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Biochemical and phylogenetic characterization of the wastewater tolerant Chlamydomonas biconvexa Embrapa|LBA40 strain cultivated in palm oil mill effluent. |
title |
Biochemical and phylogenetic characterization of the wastewater tolerant Chlamydomonas biconvexa Embrapa|LBA40 strain cultivated in palm oil mill effluent. |
spellingShingle |
Biochemical and phylogenetic characterization of the wastewater tolerant Chlamydomonas biconvexa Embrapa|LBA40 strain cultivated in palm oil mill effluent. PASCOAL, P. V. Chlamydomonas biconvexa Embrapa|LBA40 Biomassa Palm oils Microbial biomass Microbial genetics Wastewater |
title_short |
Biochemical and phylogenetic characterization of the wastewater tolerant Chlamydomonas biconvexa Embrapa|LBA40 strain cultivated in palm oil mill effluent. |
title_full |
Biochemical and phylogenetic characterization of the wastewater tolerant Chlamydomonas biconvexa Embrapa|LBA40 strain cultivated in palm oil mill effluent. |
title_fullStr |
Biochemical and phylogenetic characterization of the wastewater tolerant Chlamydomonas biconvexa Embrapa|LBA40 strain cultivated in palm oil mill effluent. |
title_full_unstemmed |
Biochemical and phylogenetic characterization of the wastewater tolerant Chlamydomonas biconvexa Embrapa|LBA40 strain cultivated in palm oil mill effluent. |
title_sort |
Biochemical and phylogenetic characterization of the wastewater tolerant Chlamydomonas biconvexa Embrapa|LBA40 strain cultivated in palm oil mill effluent. |
author |
PASCOAL, P. V. |
author_facet |
PASCOAL, P. V. RIBEIRO, D. M. CEREIJO, C. R. SANTANA, H. NASCIMENTO, R. C. STEINDORF, A. S. GARCIA, L. C. FORMIGHIERI, E. F. BRASIL, B. dos S. A. F. |
author_role |
author |
author2 |
RIBEIRO, D. M. CEREIJO, C. R. SANTANA, H. NASCIMENTO, R. C. STEINDORF, A. S. GARCIA, L. C. FORMIGHIERI, E. F. BRASIL, B. dos S. A. F. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
PATRÍCIA VERDUGO PASCOAL, Universidade Federal da Bahia DÁGON MANOEL RIBEIRO, Universidade de Brasília CAROLINA RIBEIRO CEREIJO HUGO SANTANA RODRIGO CARVALHO NASCIMENTO ANDREI STECCA STEINDORF LORENA COSTA GARCIA CALSING, CNPAE EDUARDO FERNANDES FORMIGHIERI, CNPAE BRUNO DOS SANTOS A F BRASIL, SPD. |
dc.contributor.author.fl_str_mv |
PASCOAL, P. V. RIBEIRO, D. M. CEREIJO, C. R. SANTANA, H. NASCIMENTO, R. C. STEINDORF, A. S. GARCIA, L. C. FORMIGHIERI, E. F. BRASIL, B. dos S. A. F. |
dc.subject.por.fl_str_mv |
Chlamydomonas biconvexa Embrapa|LBA40 Biomassa Palm oils Microbial biomass Microbial genetics Wastewater |
topic |
Chlamydomonas biconvexa Embrapa|LBA40 Biomassa Palm oils Microbial biomass Microbial genetics Wastewater |
description |
The increasing demand for water, food and energy poses challenges for the world´s sustainability. Tropical palm oil is currently the major source of vegetable oil worldwide with a production that exceeds 55 million tons per year, while generating over 200 million tons of palm oil mill effluent (POME). It could potentially be used as a substrate for production of microalgal biomass though. In this study, the microalgal strain Chlamydomonas biconvexa Embrapa|LBA40, originally isolated from a sugarcane vinasse stabilization pond, was selected among 17 strains tested for growth in POME retrieved from anaerobic ponds of a palm oil industrial plant located within the Amazon rainforest region. During cultivation in POME, C. biconvexa Embrapa|LBA40 biomass productivity reached 190.60 mgDW ? L-1 ? d-1 using 15L airlift flat plate photobioreactors. Carbohydrates comprised the major fraction of algal biomass (31.96%), while the lipidic fraction reached up to 11.3% of dry mass. Reductions of 99% in ammonium and nitrite, as well as 98% reduction in phosphate present in POME were detected after 5 days of algal cultivation. This suggests that the aerobic pond stage, usually used in palm oil industrial plants to reduce POME inorganic load, could be substituted by high rate photobioreactors, significantly reducing the time and area requirements for wastewater treatment. In addition, the complete mitochondrial genome of C. biconvexa Embrapa|LBA40 strain was sequenced, revealing a compact mitogenome, with 15.98 kb in size, a total of 14 genes, of which 9 are protein coding genes. Phylogenetic analysis confirmed the strain taxonomic status within the Chlamydomonas genus, opening up opportunities for future genetic modification and molecular breeding programs in these species. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04-19T19:33:52Z 2021-04-19T19:33:52Z 2021-04-19 2021 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PLoS One, v. 16, n. 4, e0249089, 2021. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131384 https://doi.org/10.1371/journal.pone.0249089 |
identifier_str_mv |
PLoS One, v. 16, n. 4, e0249089, 2021. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131384 https://doi.org/10.1371/journal.pone.0249089 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503504435347456 |