An approach on the in vitro maintenance of sugarcane with views for conservation and monitoring of plant nuclear DNA contents via flow cytometry.
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1023802 |
Resumo: | In vitro conservation techniques can be utilized for germplasm maintenance. However, few reports on the in vitro conservation of sugarcane species are present in the literature. The objective of this study was to subject sugarcane plants to in vitro under minimal growth conditions and to evaluate the survival, regeneration, and the monitoring of nuclear DNA content levels of the plants. Shoots from 10 sugarcane varie-ties (Saccharum spp.) were introduced into two media: MC1, consisting of half-strength Murashige and Skoog (MS) salts and 3% sorbitol, or MC2, similar to the first formulation, but additionally supplemented with 3.8 ?Mabscisicacid(ABA). |
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An approach on the in vitro maintenance of sugarcane with views for conservation and monitoring of plant nuclear DNA contents via flow cytometry.Cana de açúcarFlow cytometrySugarcaneIn vitro conservation techniques can be utilized for germplasm maintenance. However, few reports on the in vitro conservation of sugarcane species are present in the literature. The objective of this study was to subject sugarcane plants to in vitro under minimal growth conditions and to evaluate the survival, regeneration, and the monitoring of nuclear DNA content levels of the plants. Shoots from 10 sugarcane varie-ties (Saccharum spp.) were introduced into two media: MC1, consisting of half-strength Murashige and Skoog (MS) salts and 3% sorbitol, or MC2, similar to the first formulation, but additionally supplemented with 3.8 ?Mabscisicacid(ABA).GABRIELA FERREIRA NOGUEIRA; LEILA APARECIDA SALLES PIO, UFLA; MOACIR PASQUAL, UFLA; ADRIANE LEITE DO AMARAL, CPATC; JONNY EVERSON SCHERWINSKI PEREIRA, CENARGEN.NOGUEIRA, G. F.PIO, L. A. S.PASQUAL, M.AMARAL, A. L. doCHERWINSKI-PEREIRA, J. E.2018-07-19T01:01:18Z2018-07-19T01:01:18Z2015-09-1420152018-07-19T01:01:18Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleIn Vitro Cellular & Developmental Biology. Plant, v. 51, p. 220-230, 2015.http://www.alice.cnptia.embrapa.br/alice/handle/doc/102380210.1007/s11627-014-9660-yenginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-07-19T01:01:25Zoai:www.alice.cnptia.embrapa.br:doc/1023802Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-07-19T01:01:25falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-07-19T01:01:25Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
An approach on the in vitro maintenance of sugarcane with views for conservation and monitoring of plant nuclear DNA contents via flow cytometry. |
title |
An approach on the in vitro maintenance of sugarcane with views for conservation and monitoring of plant nuclear DNA contents via flow cytometry. |
spellingShingle |
An approach on the in vitro maintenance of sugarcane with views for conservation and monitoring of plant nuclear DNA contents via flow cytometry. NOGUEIRA, G. F. Cana de açúcar Flow cytometry Sugarcane |
title_short |
An approach on the in vitro maintenance of sugarcane with views for conservation and monitoring of plant nuclear DNA contents via flow cytometry. |
title_full |
An approach on the in vitro maintenance of sugarcane with views for conservation and monitoring of plant nuclear DNA contents via flow cytometry. |
title_fullStr |
An approach on the in vitro maintenance of sugarcane with views for conservation and monitoring of plant nuclear DNA contents via flow cytometry. |
title_full_unstemmed |
An approach on the in vitro maintenance of sugarcane with views for conservation and monitoring of plant nuclear DNA contents via flow cytometry. |
title_sort |
An approach on the in vitro maintenance of sugarcane with views for conservation and monitoring of plant nuclear DNA contents via flow cytometry. |
author |
NOGUEIRA, G. F. |
author_facet |
NOGUEIRA, G. F. PIO, L. A. S. PASQUAL, M. AMARAL, A. L. do CHERWINSKI-PEREIRA, J. E. |
author_role |
author |
author2 |
PIO, L. A. S. PASQUAL, M. AMARAL, A. L. do CHERWINSKI-PEREIRA, J. E. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
GABRIELA FERREIRA NOGUEIRA; LEILA APARECIDA SALLES PIO, UFLA; MOACIR PASQUAL, UFLA; ADRIANE LEITE DO AMARAL, CPATC; JONNY EVERSON SCHERWINSKI PEREIRA, CENARGEN. |
dc.contributor.author.fl_str_mv |
NOGUEIRA, G. F. PIO, L. A. S. PASQUAL, M. AMARAL, A. L. do CHERWINSKI-PEREIRA, J. E. |
dc.subject.por.fl_str_mv |
Cana de açúcar Flow cytometry Sugarcane |
topic |
Cana de açúcar Flow cytometry Sugarcane |
description |
In vitro conservation techniques can be utilized for germplasm maintenance. However, few reports on the in vitro conservation of sugarcane species are present in the literature. The objective of this study was to subject sugarcane plants to in vitro under minimal growth conditions and to evaluate the survival, regeneration, and the monitoring of nuclear DNA content levels of the plants. Shoots from 10 sugarcane varie-ties (Saccharum spp.) were introduced into two media: MC1, consisting of half-strength Murashige and Skoog (MS) salts and 3% sorbitol, or MC2, similar to the first formulation, but additionally supplemented with 3.8 ?Mabscisicacid(ABA). |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-14 2015 2018-07-19T01:01:18Z 2018-07-19T01:01:18Z 2018-07-19T01:01:18Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
In Vitro Cellular & Developmental Biology. Plant, v. 51, p. 220-230, 2015. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1023802 10.1007/s11627-014-9660-y |
identifier_str_mv |
In Vitro Cellular & Developmental Biology. Plant, v. 51, p. 220-230, 2015. 10.1007/s11627-014-9660-y |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1023802 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503459082338304 |