Expression patterns of three a-expansin isoforms in Coffea arabica during fruit development.
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/880493 |
Resumo: | As a first step towards understanding the physiological role and regulation of the expansin gene (EXP) family in Coffea arabica fruits during growth and maturation, we identified 11 expansin genes, nine belonging to the expansin family (EXPA), one EXLA and one EXLB, through in silico analysis of expressed sequence tags (ESTs). Within the expansin family, three isoforms were selected for detailed examination based on their high expression in coffee fruits or because they were specifically induced during different fruit developmental stages, according to the EST information. The expression patterns were analysed in different fruit tissues (perisperm, endosperm and pericarp) of C. arabica cv. IAPAR-59 and C. arabica cv. IAPAR-59 Grau´ do, the latter being a closely related cultivar with a larger fruit size. Accumulation of CaEXPA1 and CaEXPA3 transcripts was high in the perisperm (tissue responsible for coffee bean size) and in the early stages of pericarp development. Transcripts of CaEXPA2 were detected only in the pericarp during the later stages of fruit maturation and ripening. There was no detectable transcription of the three EXPs analysed in the endosperm. The observed differences in mRNA expression levels of CaEXPA1 and CaEXP3 in the perisperm of IAPAR-59 and IAPAR-59 Grau´do suggest the participation of these two isoforms in the regulation of grain size. |
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Expression patterns of three a-expansin isoforms in Coffea arabica during fruit development.PerispermCoffeeExpasion isoformsFruit ripeningGrain qualityAs a first step towards understanding the physiological role and regulation of the expansin gene (EXP) family in Coffea arabica fruits during growth and maturation, we identified 11 expansin genes, nine belonging to the expansin family (EXPA), one EXLA and one EXLB, through in silico analysis of expressed sequence tags (ESTs). Within the expansin family, three isoforms were selected for detailed examination based on their high expression in coffee fruits or because they were specifically induced during different fruit developmental stages, according to the EST information. The expression patterns were analysed in different fruit tissues (perisperm, endosperm and pericarp) of C. arabica cv. IAPAR-59 and C. arabica cv. IAPAR-59 Grau´ do, the latter being a closely related cultivar with a larger fruit size. Accumulation of CaEXPA1 and CaEXPA3 transcripts was high in the perisperm (tissue responsible for coffee bean size) and in the early stages of pericarp development. Transcripts of CaEXPA2 were detected only in the pericarp during the later stages of fruit maturation and ripening. There was no detectable transcription of the three EXPs analysed in the endosperm. The observed differences in mRNA expression levels of CaEXPA1 and CaEXP3 in the perisperm of IAPAR-59 and IAPAR-59 Grau´do suggest the participation of these two isoforms in the regulation of grain size.IAPAR; IAPAR/Universidade Estadual de Londrina; IAPAR; CIRAD/UMR/DAP; IAPAR; LUIZ FILIPE PROTASIO PEREIRA, SAPC.BUDZINSKI, I. G. F.SANTOS, T. B.SERA, T.POT, D.VIEIRA, L. G. E.PEREIRA, L. F. P.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2011-03-1120112011-10-04T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlant Biology, Germany, v.13, n. 3, p. 462?471, 2011.http://www.alice.cnptia.embrapa.br/alice/handle/doc/880493enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:33:40Zoai:www.alice.cnptia.embrapa.br:doc/880493Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T22:33:40falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:33:40Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Expression patterns of three a-expansin isoforms in Coffea arabica during fruit development. |
title |
Expression patterns of three a-expansin isoforms in Coffea arabica during fruit development. |
spellingShingle |
Expression patterns of three a-expansin isoforms in Coffea arabica during fruit development. BUDZINSKI, I. G. F. Perisperm Coffee Expasion isoforms Fruit ripening Grain quality |
title_short |
Expression patterns of three a-expansin isoforms in Coffea arabica during fruit development. |
title_full |
Expression patterns of three a-expansin isoforms in Coffea arabica during fruit development. |
title_fullStr |
Expression patterns of three a-expansin isoforms in Coffea arabica during fruit development. |
title_full_unstemmed |
Expression patterns of three a-expansin isoforms in Coffea arabica during fruit development. |
title_sort |
Expression patterns of three a-expansin isoforms in Coffea arabica during fruit development. |
author |
BUDZINSKI, I. G. F. |
author_facet |
BUDZINSKI, I. G. F. SANTOS, T. B. SERA, T. POT, D. VIEIRA, L. G. E. PEREIRA, L. F. P. |
author_role |
author |
author2 |
SANTOS, T. B. SERA, T. POT, D. VIEIRA, L. G. E. PEREIRA, L. F. P. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
IAPAR; IAPAR/Universidade Estadual de Londrina; IAPAR; CIRAD/UMR/DAP; IAPAR; LUIZ FILIPE PROTASIO PEREIRA, SAPC. |
dc.contributor.author.fl_str_mv |
BUDZINSKI, I. G. F. SANTOS, T. B. SERA, T. POT, D. VIEIRA, L. G. E. PEREIRA, L. F. P. |
dc.subject.por.fl_str_mv |
Perisperm Coffee Expasion isoforms Fruit ripening Grain quality |
topic |
Perisperm Coffee Expasion isoforms Fruit ripening Grain quality |
description |
As a first step towards understanding the physiological role and regulation of the expansin gene (EXP) family in Coffea arabica fruits during growth and maturation, we identified 11 expansin genes, nine belonging to the expansin family (EXPA), one EXLA and one EXLB, through in silico analysis of expressed sequence tags (ESTs). Within the expansin family, three isoforms were selected for detailed examination based on their high expression in coffee fruits or because they were specifically induced during different fruit developmental stages, according to the EST information. The expression patterns were analysed in different fruit tissues (perisperm, endosperm and pericarp) of C. arabica cv. IAPAR-59 and C. arabica cv. IAPAR-59 Grau´ do, the latter being a closely related cultivar with a larger fruit size. Accumulation of CaEXPA1 and CaEXPA3 transcripts was high in the perisperm (tissue responsible for coffee bean size) and in the early stages of pericarp development. Transcripts of CaEXPA2 were detected only in the pericarp during the later stages of fruit maturation and ripening. There was no detectable transcription of the three EXPs analysed in the endosperm. The observed differences in mRNA expression levels of CaEXPA1 and CaEXP3 in the perisperm of IAPAR-59 and IAPAR-59 Grau´do suggest the participation of these two isoforms in the regulation of grain size. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2011-03-11 2011 2011-10-04T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Plant Biology, Germany, v.13, n. 3, p. 462?471, 2011. http://www.alice.cnptia.embrapa.br/alice/handle/doc/880493 |
identifier_str_mv |
Plant Biology, Germany, v.13, n. 3, p. 462?471, 2011. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/880493 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503340802965504 |