Ovarian Grafts 10 days after Xenotransplantation: folliculogenesis and recovery of viable oocytes.

Detalhes bibliográficos
Autor(a) principal: CAMPOS-JUNIOR, P. H. A.
Data de Publicação: 2016
Outros Autores: ALVES, T. J. M., DIAS, M. T., ASSUNÇAO, C. M., MUNK, M., MATTOS, M. S., KRAEMER, L. R., ALMEIDA, B. G., RUSSO, R. C., BARCELOS, L., CAMARGO, L. S. de A., VIANA, J. H. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1062986
Resumo: Abstracts Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in the grafts, as indicated by the presence of primordial, primary, secondary, antral, and atretic follicles. The xenografts showed a greater volumetric density of atretic follicles and higher hyperemia and number of host-derived macrophage and neutrophil (P<0.05), when compared to non-grafted fragments. There was a higher blood perfusion under the back skin in the transplantation sites of host animals than in control and non-grafted (P<0.01). BAX and PRDX1 genes were up-regulated, while BCL2, FSHR, IGF1R and IGF2R were down-regulated, when compared to the control (P<0.01). Twenty seven oocytes were successfully harvested from grafts, and some of these oocytes were able to give rise to blastocysts after in vitro fertilization. However, cleavage and blastocyst rates of xenograft derived oocytes were lower than in control (P<0.01). Despite showing some functional modifications, the ovarian xenografts were able to support folliculogenesis and produce functional oocytes.
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spelling Ovarian Grafts 10 days after Xenotransplantation: folliculogenesis and recovery of viable oocytes.Bovine ovarian graftsOvarian xenograftsoocytesAbstracts Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in the grafts, as indicated by the presence of primordial, primary, secondary, antral, and atretic follicles. The xenografts showed a greater volumetric density of atretic follicles and higher hyperemia and number of host-derived macrophage and neutrophil (P<0.05), when compared to non-grafted fragments. There was a higher blood perfusion under the back skin in the transplantation sites of host animals than in control and non-grafted (P<0.01). BAX and PRDX1 genes were up-regulated, while BCL2, FSHR, IGF1R and IGF2R were down-regulated, when compared to the control (P<0.01). Twenty seven oocytes were successfully harvested from grafts, and some of these oocytes were able to give rise to blastocysts after in vitro fertilization. However, cleavage and blastocyst rates of xenograft derived oocytes were lower than in control (P<0.01). Despite showing some functional modifications, the ovarian xenografts were able to support folliculogenesis and produce functional oocytes.PAULO HENRIQUE ALMEIDA CAMPOS-JUNIOR, UFSJ; THALYS JAIR MELO ALVES, UFSJ; MARCO TULIO DIAS, UFSJ; CAROLINA MARINHO ASSUNÇAO, UFJF; MICHELE MUNK, UFJF; MATHEUS SILVÉRIO MATTOS, UFMG; LUCAS ROCHA KRAEMER, UFMG; BRÍGIDA GOMES ALMEIDA, UFMG; REMO CASTRO RUSSO, UFMG; LUCÍOLA BARCELOS, UFMG; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen.CAMPOS-JUNIOR, P. H. A.ALVES, T. J. M.DIAS, M. T.ASSUNÇAO, C. M.MUNK, M.MATTOS, M. S.KRAEMER, L. R.ALMEIDA, B. G.RUSSO, R. C.BARCELOS, L.CAMARGO, L. S. de A.VIANA, J. H. M.2017-02-07T11:11:11Z2017-02-07T11:11:11Z2017-02-0720162017-02-15T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlos One, v. 11, n. 6, e0158109, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/106298610.1371/journal. pone.0158109enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T04:08:53Zoai:www.alice.cnptia.embrapa.br:doc/1062986Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:08:53Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Ovarian Grafts 10 days after Xenotransplantation: folliculogenesis and recovery of viable oocytes.
title Ovarian Grafts 10 days after Xenotransplantation: folliculogenesis and recovery of viable oocytes.
spellingShingle Ovarian Grafts 10 days after Xenotransplantation: folliculogenesis and recovery of viable oocytes.
CAMPOS-JUNIOR, P. H. A.
Bovine ovarian grafts
Ovarian xenografts
oocytes
title_short Ovarian Grafts 10 days after Xenotransplantation: folliculogenesis and recovery of viable oocytes.
title_full Ovarian Grafts 10 days after Xenotransplantation: folliculogenesis and recovery of viable oocytes.
title_fullStr Ovarian Grafts 10 days after Xenotransplantation: folliculogenesis and recovery of viable oocytes.
title_full_unstemmed Ovarian Grafts 10 days after Xenotransplantation: folliculogenesis and recovery of viable oocytes.
title_sort Ovarian Grafts 10 days after Xenotransplantation: folliculogenesis and recovery of viable oocytes.
author CAMPOS-JUNIOR, P. H. A.
author_facet CAMPOS-JUNIOR, P. H. A.
ALVES, T. J. M.
DIAS, M. T.
ASSUNÇAO, C. M.
MUNK, M.
MATTOS, M. S.
KRAEMER, L. R.
ALMEIDA, B. G.
RUSSO, R. C.
BARCELOS, L.
CAMARGO, L. S. de A.
VIANA, J. H. M.
author_role author
author2 ALVES, T. J. M.
DIAS, M. T.
ASSUNÇAO, C. M.
MUNK, M.
MATTOS, M. S.
KRAEMER, L. R.
ALMEIDA, B. G.
RUSSO, R. C.
BARCELOS, L.
CAMARGO, L. S. de A.
VIANA, J. H. M.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv PAULO HENRIQUE ALMEIDA CAMPOS-JUNIOR, UFSJ; THALYS JAIR MELO ALVES, UFSJ; MARCO TULIO DIAS, UFSJ; CAROLINA MARINHO ASSUNÇAO, UFJF; MICHELE MUNK, UFJF; MATHEUS SILVÉRIO MATTOS, UFMG; LUCAS ROCHA KRAEMER, UFMG; BRÍGIDA GOMES ALMEIDA, UFMG; REMO CASTRO RUSSO, UFMG; LUCÍOLA BARCELOS, UFMG; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen.
dc.contributor.author.fl_str_mv CAMPOS-JUNIOR, P. H. A.
ALVES, T. J. M.
DIAS, M. T.
ASSUNÇAO, C. M.
MUNK, M.
MATTOS, M. S.
KRAEMER, L. R.
ALMEIDA, B. G.
RUSSO, R. C.
BARCELOS, L.
CAMARGO, L. S. de A.
VIANA, J. H. M.
dc.subject.por.fl_str_mv Bovine ovarian grafts
Ovarian xenografts
oocytes
topic Bovine ovarian grafts
Ovarian xenografts
oocytes
description Abstracts Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in the grafts, as indicated by the presence of primordial, primary, secondary, antral, and atretic follicles. The xenografts showed a greater volumetric density of atretic follicles and higher hyperemia and number of host-derived macrophage and neutrophil (P<0.05), when compared to non-grafted fragments. There was a higher blood perfusion under the back skin in the transplantation sites of host animals than in control and non-grafted (P<0.01). BAX and PRDX1 genes were up-regulated, while BCL2, FSHR, IGF1R and IGF2R were down-regulated, when compared to the control (P<0.01). Twenty seven oocytes were successfully harvested from grafts, and some of these oocytes were able to give rise to blastocysts after in vitro fertilization. However, cleavage and blastocyst rates of xenograft derived oocytes were lower than in control (P<0.01). Despite showing some functional modifications, the ovarian xenografts were able to support folliculogenesis and produce functional oocytes.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-02-07T11:11:11Z
2017-02-07T11:11:11Z
2017-02-07
2017-02-15T11:11:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Plos One, v. 11, n. 6, e0158109, 2016.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1062986
10.1371/journal. pone.0158109
identifier_str_mv Plos One, v. 11, n. 6, e0158109, 2016.
10.1371/journal. pone.0158109
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1062986
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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