In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Fitos |
Texto Completo: | https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/620 |
Resumo: | Psychotria ipecacuanha is a medicinal plant that is native to the Americas with the largest area of occurrence in the State of Mato Grosso in Brazil. It is critically endangered due to overexploitation of natural populations. Due to difficulties in conventional propagation, the aim of this study was to evaluate the effect of different concentrations of the growth regulator indoleacetic acid (IAA) on in vitro propagation of this species. Stem node explants were cultured in Murashige and Skoog medium (MS) without growth regulators (control) and supplemented with four concentrations (0.05, 0.5, 1.5 and 2.0 mg.L-1) of indoleacetic acid (IAA) in semisolid media. After 60 days of cultivation, nodal segments (n=30) grown in Murashige & Skoog (MS) medium supplemented with 0.05 mg.L-1 of IAA produced on average 4.56 nodal segments per explant. The seedlings were successfully acclimatized without detection of any morphological anomalies or variation. |
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In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic AcidPropagação in vitro de Psychotria ipecacuanha (Brot.) Stokes sob a ação de diferentes concentrações de Ácido IndolacéticoPlant biotechnology. tissue culture. micropropagation. Rubiaceae. ipecac.Psychotria ipecacuanha is a medicinal plant that is native to the Americas with the largest area of occurrence in the State of Mato Grosso in Brazil. It is critically endangered due to overexploitation of natural populations. Due to difficulties in conventional propagation, the aim of this study was to evaluate the effect of different concentrations of the growth regulator indoleacetic acid (IAA) on in vitro propagation of this species. Stem node explants were cultured in Murashige and Skoog medium (MS) without growth regulators (control) and supplemented with four concentrations (0.05, 0.5, 1.5 and 2.0 mg.L-1) of indoleacetic acid (IAA) in semisolid media. After 60 days of cultivation, nodal segments (n=30) grown in Murashige & Skoog (MS) medium supplemented with 0.05 mg.L-1 of IAA produced on average 4.56 nodal segments per explant. The seedlings were successfully acclimatized without detection of any morphological anomalies or variation.Psychotria ipecacuanha, é uma planta medicinal nativa da América com a maior área de ocorrência no Estado de Mato Grosso - Brasil, críticamente ameaçada devido à sobreexploração das populações naturais. Devido à dificuldades na propagação convencional, o objetivo deste estudo foi a avaliação do efeito de diferentes concentrações do regulador de crescimento ácido indolacético (AIA) na propagação in vitro de P. ipecacuanha. Segmentos nodais foram cultivados em meio de Murashige e Skoog (MS) sem reguladores de crescimento (controle) e suplementado com quatro concentrações (0,05; 0,5; 1,5 e 2,0 mg.L-1) de AIA, em meios semi-sólidos. Após 60 dias de cultivo, segmentos nodais (n=30) cultivados em meio de Murashige & Skoog (MS) suplementado com 0,05 mg.L-1 de AIA produziram, em média, 4,56 segmentos nodais por explante, cujas plântulas foram aclimatizadas com sucesso, sem exibição de qualquer anomalia morfológica ou variação.Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz2018-10-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/62010.17648/2446-4775.2018.620Revista Fitos; Vol. 12 No. 3 (2018); 263-268Revista Fitos; Vol. 12 Núm. 3 (2018); 263-268Revista Fitos; v. 12 n. 3 (2018); 263-2682446-47751808-9569reponame:Revista Fitosinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZenghttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/620/pdfhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/620/htmlCopyright (c) 2018 Revista Fitos Eletrônicainfo:eu-repo/semantics/openAccessda Silva, SimonePinheiro, Ester NetaAssunção, Laís MedeirosSilva, Efigênia LopesRodrigues, Daniele CarvalhoFerreira, Flávio FreiresMarinho, Vitor Rafael PereiraAstolfi-Filho, Spartaco2021-01-29T13:21:14Zoai:ojs.revistafitos.far.fiocruz.br:article/620Revistahttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/PUBhttp://revistafitos.far.fiocruz.br/index.php/revista-fitos/oairevistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br2446-47752446-4775opendoar:2021-01-29T13:21:14Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.none.fl_str_mv |
In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid Propagação in vitro de Psychotria ipecacuanha (Brot.) Stokes sob a ação de diferentes concentrações de Ácido Indolacético |
title |
In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid |
spellingShingle |
In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid da Silva, Simone Plant biotechnology. tissue culture. micropropagation. Rubiaceae. ipecac. |
title_short |
In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid |
title_full |
In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid |
title_fullStr |
In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid |
title_full_unstemmed |
In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid |
title_sort |
In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid |
author |
da Silva, Simone |
author_facet |
da Silva, Simone Pinheiro, Ester Neta Assunção, Laís Medeiros Silva, Efigênia Lopes Rodrigues, Daniele Carvalho Ferreira, Flávio Freires Marinho, Vitor Rafael Pereira Astolfi-Filho, Spartaco |
author_role |
author |
author2 |
Pinheiro, Ester Neta Assunção, Laís Medeiros Silva, Efigênia Lopes Rodrigues, Daniele Carvalho Ferreira, Flávio Freires Marinho, Vitor Rafael Pereira Astolfi-Filho, Spartaco |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
da Silva, Simone Pinheiro, Ester Neta Assunção, Laís Medeiros Silva, Efigênia Lopes Rodrigues, Daniele Carvalho Ferreira, Flávio Freires Marinho, Vitor Rafael Pereira Astolfi-Filho, Spartaco |
dc.subject.por.fl_str_mv |
Plant biotechnology. tissue culture. micropropagation. Rubiaceae. ipecac. |
topic |
Plant biotechnology. tissue culture. micropropagation. Rubiaceae. ipecac. |
description |
Psychotria ipecacuanha is a medicinal plant that is native to the Americas with the largest area of occurrence in the State of Mato Grosso in Brazil. It is critically endangered due to overexploitation of natural populations. Due to difficulties in conventional propagation, the aim of this study was to evaluate the effect of different concentrations of the growth regulator indoleacetic acid (IAA) on in vitro propagation of this species. Stem node explants were cultured in Murashige and Skoog medium (MS) without growth regulators (control) and supplemented with four concentrations (0.05, 0.5, 1.5 and 2.0 mg.L-1) of indoleacetic acid (IAA) in semisolid media. After 60 days of cultivation, nodal segments (n=30) grown in Murashige & Skoog (MS) medium supplemented with 0.05 mg.L-1 of IAA produced on average 4.56 nodal segments per explant. The seedlings were successfully acclimatized without detection of any morphological anomalies or variation. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/620 10.17648/2446-4775.2018.620 |
url |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/620 |
identifier_str_mv |
10.17648/2446-4775.2018.620 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/620/pdf https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/620/html |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista Fitos Eletrônica info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista Fitos Eletrônica |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz |
publisher.none.fl_str_mv |
Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz |
dc.source.none.fl_str_mv |
Revista Fitos; Vol. 12 No. 3 (2018); 263-268 Revista Fitos; Vol. 12 Núm. 3 (2018); 263-268 Revista Fitos; v. 12 n. 3 (2018); 263-268 2446-4775 1808-9569 reponame:Revista Fitos instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Revista Fitos |
collection |
Revista Fitos |
repository.name.fl_str_mv |
Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
revistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br |
_version_ |
1798313474723414016 |