Development of enzyme-linked immunosorbent assays based on recombinant MSP1a and MSP2 of Anaplasma marginale

• Autor(a) principal: Araújo,Flábio R
• Data de Publicação: 2005
• Outros Autores: Melo,Valeska SP, Ramos,Carlos AN, Madruga,Claudio R, Soares,Cleber O, Kessler,Raul H, Almeida,Nalvo F, Araújo,Graziela S, Alves,Leucio C, Torres Júnior,Roberto AA, Fragoso,Stênio P, Arauco,Paulo RC, Bacanelli,Gisele, Oliveira,Maristeli B, Santos,Lenita R
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Memórias do Instituto Oswaldo Cruz
• Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000700016
• topic: Anaplasma marginale; indirect enzyme-linked immunosorbent assays; major surface proteins; Brazil

Indirect enzyme-linked immunosorbent assays (ELISAs) based on recombinant MSP1a and MSP2 from a Brazilian isolate of Anaplasma marginale were developed to detect antibodies against this rickettsia in cattle. The high sensitivities (99% for both tests) and specificities (100% for both tests) were confirmed with sera from cattle positive or negative for A. marginale antibodies, respectively, by immunofluorescent antibody test. By the analysis of 583 sera from cattle of three regions of the state of Pernambuco, Brazil, the agreement between both tests was high, with a kappa index of 0.89. The similar performances of the ELISAs suggest that both tests can be used in epidemiological surveys for detection of antibodies to A. marginale in cattle.