Technique for the detection of Toxoplasma gondii antigens in mouse urine

Detalhes bibliográficos
Autor(a) principal: Fachado,Alberto
Data de Publicação: 1990
Outros Autores: Fonte,Luis, Rojas,Lazara, Alberti,Esteban, Machin,Rodolfo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000100010
Resumo: A simple and rapid staphylococcal coagglutination test for the detection of Toxoplasma gondii antigens in mice urine is described. A suspension of protein-A containing Staphylococcus aureus coated with rabbit hyperimmune serum was used as reagent. The sensitivity of the antigen assay was found to be at least 118 ng of the antigen protein per ml. No coagglutination was observed when the reagent was challenged against antigenic solutions of other parasites. The suitability of the method for detecting antigens of T. gondii in urine samples was studied by experimental toxoplasma infection in mice. Before the staphylococcal test, the urine samples were double serially diluted in 0.1 M PBS. From the second day on all samples from infected mice were positive at 1/16 dilution. At this dilution, all samples from non infected mice were negative or did not produce coagglutination. This method might be used in the rapid etiological diagnosis also in human cases of acute toxoplasmosis.
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spelling Technique for the detection of Toxoplasma gondii antigens in mouse urineacute toxoplasmosisToxoplasma gondiiantigenscoagglutination testA simple and rapid staphylococcal coagglutination test for the detection of Toxoplasma gondii antigens in mice urine is described. A suspension of protein-A containing Staphylococcus aureus coated with rabbit hyperimmune serum was used as reagent. The sensitivity of the antigen assay was found to be at least 118 ng of the antigen protein per ml. No coagglutination was observed when the reagent was challenged against antigenic solutions of other parasites. The suitability of the method for detecting antigens of T. gondii in urine samples was studied by experimental toxoplasma infection in mice. Before the staphylococcal test, the urine samples were double serially diluted in 0.1 M PBS. From the second day on all samples from infected mice were positive at 1/16 dilution. At this dilution, all samples from non infected mice were negative or did not produce coagglutination. This method might be used in the rapid etiological diagnosis also in human cases of acute toxoplasmosis.Instituto Oswaldo Cruz, Ministério da Saúde1990-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000100010Memórias do Instituto Oswaldo Cruz v.85 n.1 1990reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761990000100010info:eu-repo/semantics/openAccessFachado,AlbertoFonte,LuisRojas,LazaraAlberti,EstebanMachin,Rodolfoeng2020-04-25T17:46:28Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:03:38.416Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Technique for the detection of Toxoplasma gondii antigens in mouse urine
title Technique for the detection of Toxoplasma gondii antigens in mouse urine
spellingShingle Technique for the detection of Toxoplasma gondii antigens in mouse urine
Fachado,Alberto
acute toxoplasmosis
Toxoplasma gondii
antigens
coagglutination test
title_short Technique for the detection of Toxoplasma gondii antigens in mouse urine
title_full Technique for the detection of Toxoplasma gondii antigens in mouse urine
title_fullStr Technique for the detection of Toxoplasma gondii antigens in mouse urine
title_full_unstemmed Technique for the detection of Toxoplasma gondii antigens in mouse urine
title_sort Technique for the detection of Toxoplasma gondii antigens in mouse urine
author Fachado,Alberto
author_facet Fachado,Alberto
Fonte,Luis
Rojas,Lazara
Alberti,Esteban
Machin,Rodolfo
author_role author
author2 Fonte,Luis
Rojas,Lazara
Alberti,Esteban
Machin,Rodolfo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Fachado,Alberto
Fonte,Luis
Rojas,Lazara
Alberti,Esteban
Machin,Rodolfo
dc.subject.por.fl_str_mv acute toxoplasmosis
Toxoplasma gondii
antigens
coagglutination test
topic acute toxoplasmosis
Toxoplasma gondii
antigens
coagglutination test
dc.description.none.fl_txt_mv A simple and rapid staphylococcal coagglutination test for the detection of Toxoplasma gondii antigens in mice urine is described. A suspension of protein-A containing Staphylococcus aureus coated with rabbit hyperimmune serum was used as reagent. The sensitivity of the antigen assay was found to be at least 118 ng of the antigen protein per ml. No coagglutination was observed when the reagent was challenged against antigenic solutions of other parasites. The suitability of the method for detecting antigens of T. gondii in urine samples was studied by experimental toxoplasma infection in mice. Before the staphylococcal test, the urine samples were double serially diluted in 0.1 M PBS. From the second day on all samples from infected mice were positive at 1/16 dilution. At this dilution, all samples from non infected mice were negative or did not produce coagglutination. This method might be used in the rapid etiological diagnosis also in human cases of acute toxoplasmosis.
description A simple and rapid staphylococcal coagglutination test for the detection of Toxoplasma gondii antigens in mice urine is described. A suspension of protein-A containing Staphylococcus aureus coated with rabbit hyperimmune serum was used as reagent. The sensitivity of the antigen assay was found to be at least 118 ng of the antigen protein per ml. No coagglutination was observed when the reagent was challenged against antigenic solutions of other parasites. The suitability of the method for detecting antigens of T. gondii in urine samples was studied by experimental toxoplasma infection in mice. Before the staphylococcal test, the urine samples were double serially diluted in 0.1 M PBS. From the second day on all samples from infected mice were positive at 1/16 dilution. At this dilution, all samples from non infected mice were negative or did not produce coagglutination. This method might be used in the rapid etiological diagnosis also in human cases of acute toxoplasmosis.
publishDate 1990
dc.date.none.fl_str_mv 1990-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000100010
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000100010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761990000100010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.85 n.1 1990
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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