Neutrophil CD64 expression levels in IGRA-positive individuals distinguish latent tuberculosis from active disease
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100319 |
Resumo: | BACKGROUND CD64 (FcγR1) is a high-affinity receptor for monomeric IgG1 and IgG3. Circulating neutrophils express very low amounts of CD64 on their surface. OBJECTIVES Our primary aim was to investigate the utility of neutrophil CD64 surface expression as a biomarker of active pulmonary tuberculosis (TB). We hypothesised that elevated neutrophil CD64 expression in TB infection would be associated with interferon gamma (IFN-γ) as an inducer of CD64 expression. METHODS The expression level of CD64 per neutrophil (PMN CD64 index) was quantitatively measured with flow cytometry using a Leuko64 kit in samples from patients with TB and latent TB infection (LTBI) as well as healthy controls, as part of a prospective cohort study in Brazil. FINDINGS The PMN CD64 index in patients with TB was higher than that in healthy controls and LTBI. Receiver operating characteristic curve analyses determined that the PMN CD64 index could discriminate patients with TB from those with LTBI and healthy individuals. PMN CD64 index levels returned to baseline levels after treatment. CONCLUSIONS The positive regulation of CD64 expression in circulating neutrophils of patients with active TB could represent an additional biomarker for diagnosis of active TB and could be used for monitoring individuals with LTBI before progression of TB disease. |
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Memórias do Instituto Oswaldo Cruz |
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Neutrophil CD64 expression levels in IGRA-positive individuals distinguish latent tuberculosis from active diseasetuberculosislatent tuberculosis infectionbiomarkersneutrophilsCD64 BACKGROUND CD64 (FcγR1) is a high-affinity receptor for monomeric IgG1 and IgG3. Circulating neutrophils express very low amounts of CD64 on their surface. OBJECTIVES Our primary aim was to investigate the utility of neutrophil CD64 surface expression as a biomarker of active pulmonary tuberculosis (TB). We hypothesised that elevated neutrophil CD64 expression in TB infection would be associated with interferon gamma (IFN-γ) as an inducer of CD64 expression. METHODS The expression level of CD64 per neutrophil (PMN CD64 index) was quantitatively measured with flow cytometry using a Leuko64 kit in samples from patients with TB and latent TB infection (LTBI) as well as healthy controls, as part of a prospective cohort study in Brazil. FINDINGS The PMN CD64 index in patients with TB was higher than that in healthy controls and LTBI. Receiver operating characteristic curve analyses determined that the PMN CD64 index could discriminate patients with TB from those with LTBI and healthy individuals. PMN CD64 index levels returned to baseline levels after treatment. CONCLUSIONS The positive regulation of CD64 expression in circulating neutrophils of patients with active TB could represent an additional biomarker for diagnosis of active TB and could be used for monitoring individuals with LTBI before progression of TB disease.Instituto Oswaldo Cruz, Ministério da Saúde2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100319Memórias do Instituto Oswaldo Cruz v.114 2019reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-02760180579info:eu-repo/semantics/openAccessCorrêa,Raquel da SilvaRodrigues,Luciana SilvaPereira,Leticia Hagge LimaNogueira,Otto CastroLeung,JanaínaSousa,Marcela da SHacker,Mariana de AndreaSiqueira,Helio Ribeiro deCapone,DomênicoAlves,Rogério Lopes RufinoPessolani,Maria Cristina VidalSchmitz,VeronicaPereira,Geraldo Moura Batistaeng2020-04-25T17:52:57Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:22:32.889Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Neutrophil CD64 expression levels in IGRA-positive individuals distinguish latent tuberculosis from active disease |
title |
Neutrophil CD64 expression levels in IGRA-positive individuals distinguish latent tuberculosis from active disease |
spellingShingle |
Neutrophil CD64 expression levels in IGRA-positive individuals distinguish latent tuberculosis from active disease Corrêa,Raquel da Silva tuberculosis latent tuberculosis infection biomarkers neutrophils CD64 |
title_short |
Neutrophil CD64 expression levels in IGRA-positive individuals distinguish latent tuberculosis from active disease |
title_full |
Neutrophil CD64 expression levels in IGRA-positive individuals distinguish latent tuberculosis from active disease |
title_fullStr |
Neutrophil CD64 expression levels in IGRA-positive individuals distinguish latent tuberculosis from active disease |
title_full_unstemmed |
Neutrophil CD64 expression levels in IGRA-positive individuals distinguish latent tuberculosis from active disease |
title_sort |
Neutrophil CD64 expression levels in IGRA-positive individuals distinguish latent tuberculosis from active disease |
author |
Corrêa,Raquel da Silva |
author_facet |
Corrêa,Raquel da Silva Rodrigues,Luciana Silva Pereira,Leticia Hagge Lima Nogueira,Otto Castro Leung,Janaína Sousa,Marcela da S Hacker,Mariana de Andrea Siqueira,Helio Ribeiro de Capone,Domênico Alves,Rogério Lopes Rufino Pessolani,Maria Cristina Vidal Schmitz,Veronica Pereira,Geraldo Moura Batista |
author_role |
author |
author2 |
Rodrigues,Luciana Silva Pereira,Leticia Hagge Lima Nogueira,Otto Castro Leung,Janaína Sousa,Marcela da S Hacker,Mariana de Andrea Siqueira,Helio Ribeiro de Capone,Domênico Alves,Rogério Lopes Rufino Pessolani,Maria Cristina Vidal Schmitz,Veronica Pereira,Geraldo Moura Batista |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Corrêa,Raquel da Silva Rodrigues,Luciana Silva Pereira,Leticia Hagge Lima Nogueira,Otto Castro Leung,Janaína Sousa,Marcela da S Hacker,Mariana de Andrea Siqueira,Helio Ribeiro de Capone,Domênico Alves,Rogério Lopes Rufino Pessolani,Maria Cristina Vidal Schmitz,Veronica Pereira,Geraldo Moura Batista |
dc.subject.por.fl_str_mv |
tuberculosis latent tuberculosis infection biomarkers neutrophils CD64 |
topic |
tuberculosis latent tuberculosis infection biomarkers neutrophils CD64 |
dc.description.none.fl_txt_mv |
BACKGROUND CD64 (FcγR1) is a high-affinity receptor for monomeric IgG1 and IgG3. Circulating neutrophils express very low amounts of CD64 on their surface. OBJECTIVES Our primary aim was to investigate the utility of neutrophil CD64 surface expression as a biomarker of active pulmonary tuberculosis (TB). We hypothesised that elevated neutrophil CD64 expression in TB infection would be associated with interferon gamma (IFN-γ) as an inducer of CD64 expression. METHODS The expression level of CD64 per neutrophil (PMN CD64 index) was quantitatively measured with flow cytometry using a Leuko64 kit in samples from patients with TB and latent TB infection (LTBI) as well as healthy controls, as part of a prospective cohort study in Brazil. FINDINGS The PMN CD64 index in patients with TB was higher than that in healthy controls and LTBI. Receiver operating characteristic curve analyses determined that the PMN CD64 index could discriminate patients with TB from those with LTBI and healthy individuals. PMN CD64 index levels returned to baseline levels after treatment. CONCLUSIONS The positive regulation of CD64 expression in circulating neutrophils of patients with active TB could represent an additional biomarker for diagnosis of active TB and could be used for monitoring individuals with LTBI before progression of TB disease. |
description |
BACKGROUND CD64 (FcγR1) is a high-affinity receptor for monomeric IgG1 and IgG3. Circulating neutrophils express very low amounts of CD64 on their surface. OBJECTIVES Our primary aim was to investigate the utility of neutrophil CD64 surface expression as a biomarker of active pulmonary tuberculosis (TB). We hypothesised that elevated neutrophil CD64 expression in TB infection would be associated with interferon gamma (IFN-γ) as an inducer of CD64 expression. METHODS The expression level of CD64 per neutrophil (PMN CD64 index) was quantitatively measured with flow cytometry using a Leuko64 kit in samples from patients with TB and latent TB infection (LTBI) as well as healthy controls, as part of a prospective cohort study in Brazil. FINDINGS The PMN CD64 index in patients with TB was higher than that in healthy controls and LTBI. Receiver operating characteristic curve analyses determined that the PMN CD64 index could discriminate patients with TB from those with LTBI and healthy individuals. PMN CD64 index levels returned to baseline levels after treatment. CONCLUSIONS The positive regulation of CD64 expression in circulating neutrophils of patients with active TB could represent an additional biomarker for diagnosis of active TB and could be used for monitoring individuals with LTBI before progression of TB disease. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100319 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100319 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0074-02760180579 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.114 2019 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
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1669937727203704832 |