Detection of Lymnaea columella infection by Fasciola hepatica through Multiplex-PCR

Detalhes bibliográficos
Autor(a) principal: Magalhães,Kelly Grace
Data de Publicação: 2004
Outros Autores: Passos,Liana Konovaloff Jannotti, Carvalho,Omar dos Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000400013
Resumo: From complete mitochondrial DNA sequence of Fasciola hepatica available in Genbank, specific primers were designed for a conserved and repetitive region of this trematode. A pair of primers was used for diagnosis of infected Lymnaea columella by F. hepatica during the pre-patent period simultaneously with another pair of primers which amplified the internal transcribed spacer (ITS) region of rDNA from L. columella in a single Multiplex-PCR. The amplification generated a ladder band profile specific for F. hepatica. This profile was observed in positive molluscs at different times of infection, including adult worms from the trematode. The Multiplex-PCR technique showed to be a fast and safe tool for fascioliasis diagnosis, enabling the detection of F. hepatica miracidia in L. columella during the pre-patent period and identification of transmission areas.
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spelling Detection of Lymnaea columella infection by Fasciola hepatica through Multiplex-PCRFasciola hepaticaLymnaea columellamultiplex polymerase chain reactionDNA mitochondrialdiagnosisFrom complete mitochondrial DNA sequence of Fasciola hepatica available in Genbank, specific primers were designed for a conserved and repetitive region of this trematode. A pair of primers was used for diagnosis of infected Lymnaea columella by F. hepatica during the pre-patent period simultaneously with another pair of primers which amplified the internal transcribed spacer (ITS) region of rDNA from L. columella in a single Multiplex-PCR. The amplification generated a ladder band profile specific for F. hepatica. This profile was observed in positive molluscs at different times of infection, including adult worms from the trematode. The Multiplex-PCR technique showed to be a fast and safe tool for fascioliasis diagnosis, enabling the detection of F. hepatica miracidia in L. columella during the pre-patent period and identification of transmission areas.Instituto Oswaldo Cruz, Ministério da Saúde2004-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000400013Memórias do Instituto Oswaldo Cruz v.99 n.4 2004reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762004000400013info:eu-repo/semantics/openAccessMagalhães,Kelly GracePassos,Liana Konovaloff JannottiCarvalho,Omar dos Santoseng2020-04-25T17:49:16Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:12:39.286Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Detection of Lymnaea columella infection by Fasciola hepatica through Multiplex-PCR
title Detection of Lymnaea columella infection by Fasciola hepatica through Multiplex-PCR
spellingShingle Detection of Lymnaea columella infection by Fasciola hepatica through Multiplex-PCR
Magalhães,Kelly Grace
Fasciola hepatica
Lymnaea columella
multiplex polymerase chain reaction
DNA mitochondrial
diagnosis
title_short Detection of Lymnaea columella infection by Fasciola hepatica through Multiplex-PCR
title_full Detection of Lymnaea columella infection by Fasciola hepatica through Multiplex-PCR
title_fullStr Detection of Lymnaea columella infection by Fasciola hepatica through Multiplex-PCR
title_full_unstemmed Detection of Lymnaea columella infection by Fasciola hepatica through Multiplex-PCR
title_sort Detection of Lymnaea columella infection by Fasciola hepatica through Multiplex-PCR
author Magalhães,Kelly Grace
author_facet Magalhães,Kelly Grace
Passos,Liana Konovaloff Jannotti
Carvalho,Omar dos Santos
author_role author
author2 Passos,Liana Konovaloff Jannotti
Carvalho,Omar dos Santos
author2_role author
author
dc.contributor.author.fl_str_mv Magalhães,Kelly Grace
Passos,Liana Konovaloff Jannotti
Carvalho,Omar dos Santos
dc.subject.por.fl_str_mv Fasciola hepatica
Lymnaea columella
multiplex polymerase chain reaction
DNA mitochondrial
diagnosis
topic Fasciola hepatica
Lymnaea columella
multiplex polymerase chain reaction
DNA mitochondrial
diagnosis
dc.description.none.fl_txt_mv From complete mitochondrial DNA sequence of Fasciola hepatica available in Genbank, specific primers were designed for a conserved and repetitive region of this trematode. A pair of primers was used for diagnosis of infected Lymnaea columella by F. hepatica during the pre-patent period simultaneously with another pair of primers which amplified the internal transcribed spacer (ITS) region of rDNA from L. columella in a single Multiplex-PCR. The amplification generated a ladder band profile specific for F. hepatica. This profile was observed in positive molluscs at different times of infection, including adult worms from the trematode. The Multiplex-PCR technique showed to be a fast and safe tool for fascioliasis diagnosis, enabling the detection of F. hepatica miracidia in L. columella during the pre-patent period and identification of transmission areas.
description From complete mitochondrial DNA sequence of Fasciola hepatica available in Genbank, specific primers were designed for a conserved and repetitive region of this trematode. A pair of primers was used for diagnosis of infected Lymnaea columella by F. hepatica during the pre-patent period simultaneously with another pair of primers which amplified the internal transcribed spacer (ITS) region of rDNA from L. columella in a single Multiplex-PCR. The amplification generated a ladder band profile specific for F. hepatica. This profile was observed in positive molluscs at different times of infection, including adult worms from the trematode. The Multiplex-PCR technique showed to be a fast and safe tool for fascioliasis diagnosis, enabling the detection of F. hepatica miracidia in L. columella during the pre-patent period and identification of transmission areas.
publishDate 2004
dc.date.none.fl_str_mv 2004-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000400013
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000400013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762004000400013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.99 n.4 2004
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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