Trypanosoma cruzi: genetic group with peculiar biochemical and biological behavior
Main Author: | |
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Publication Date: | 2003 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Memórias do Instituto Oswaldo Cruz |
Download full: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500011 |
Summary: | Thirty-two Trypanosoma cruzi strains, isolated from chronic chagasic patients in the northwest of the state of Paraná (Brazil), were analyzed using molecular, biochemical and biological characteristics. Genotypic analysis using randomly amplified polymorphic DNA and simple sequence repeat-anchored polymerase chain reaction amplified profiles showed a large, genetically well-correlated group that contained the majority of the strains and a divergent group that included the PR-150 strain. For glycoconjugate composition, the PR-150 strain was different from the other strains considering the absence or presence of specific bands in aqueous or detergent phases. This strain was also totally different from the others in one out of the six parameters related to in vitro and in vivo biological behavior. We highlight the fact that the PR-150 was totally resistant to benznidazole. For the other biological parameters this strain was not totally distinct from the others, but it showed a peculiar behavior. |
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Trypanosoma cruzi: genetic group with peculiar biochemical and biological behaviorTrypanosoma cruzirandomly amplified polymorphic DNA (RAPD)simple sequence repeatanchored polymerase chain reaction amplified (SSR-PCR)glycoconjugatesbiological characteristicsThirty-two Trypanosoma cruzi strains, isolated from chronic chagasic patients in the northwest of the state of Paraná (Brazil), were analyzed using molecular, biochemical and biological characteristics. Genotypic analysis using randomly amplified polymorphic DNA and simple sequence repeat-anchored polymerase chain reaction amplified profiles showed a large, genetically well-correlated group that contained the majority of the strains and a divergent group that included the PR-150 strain. For glycoconjugate composition, the PR-150 strain was different from the other strains considering the absence or presence of specific bands in aqueous or detergent phases. This strain was also totally different from the others in one out of the six parameters related to in vitro and in vivo biological behavior. We highlight the fact that the PR-150 was totally resistant to benznidazole. For the other biological parameters this strain was not totally distinct from the others, but it showed a peculiar behavior.Instituto Oswaldo Cruz, Ministério da Saúde2003-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500011Memórias do Instituto Oswaldo Cruz v.98 n.5 2003reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762003000500011info:eu-repo/semantics/openAccessGomes,Mônica LúciaToledo,Max Jean de OrnelasNakamura,Celso VataruBittencourt,Nilza de Lucas RodriguesChiari,EglerAraújo,Silvana Marques deeng2020-04-25T17:49:06Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:12:03.926Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Trypanosoma cruzi: genetic group with peculiar biochemical and biological behavior |
title |
Trypanosoma cruzi: genetic group with peculiar biochemical and biological behavior |
spellingShingle |
Trypanosoma cruzi: genetic group with peculiar biochemical and biological behavior Gomes,Mônica Lúcia Trypanosoma cruzi randomly amplified polymorphic DNA (RAPD) simple sequence repeat anchored polymerase chain reaction amplified (SSR-PCR) glycoconjugates biological characteristics |
title_short |
Trypanosoma cruzi: genetic group with peculiar biochemical and biological behavior |
title_full |
Trypanosoma cruzi: genetic group with peculiar biochemical and biological behavior |
title_fullStr |
Trypanosoma cruzi: genetic group with peculiar biochemical and biological behavior |
title_full_unstemmed |
Trypanosoma cruzi: genetic group with peculiar biochemical and biological behavior |
title_sort |
Trypanosoma cruzi: genetic group with peculiar biochemical and biological behavior |
author |
Gomes,Mônica Lúcia |
author_facet |
Gomes,Mônica Lúcia Toledo,Max Jean de Ornelas Nakamura,Celso Vataru Bittencourt,Nilza de Lucas Rodrigues Chiari,Egler Araújo,Silvana Marques de |
author_role |
author |
author2 |
Toledo,Max Jean de Ornelas Nakamura,Celso Vataru Bittencourt,Nilza de Lucas Rodrigues Chiari,Egler Araújo,Silvana Marques de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Gomes,Mônica Lúcia Toledo,Max Jean de Ornelas Nakamura,Celso Vataru Bittencourt,Nilza de Lucas Rodrigues Chiari,Egler Araújo,Silvana Marques de |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi randomly amplified polymorphic DNA (RAPD) simple sequence repeat anchored polymerase chain reaction amplified (SSR-PCR) glycoconjugates biological characteristics |
topic |
Trypanosoma cruzi randomly amplified polymorphic DNA (RAPD) simple sequence repeat anchored polymerase chain reaction amplified (SSR-PCR) glycoconjugates biological characteristics |
dc.description.none.fl_txt_mv |
Thirty-two Trypanosoma cruzi strains, isolated from chronic chagasic patients in the northwest of the state of Paraná (Brazil), were analyzed using molecular, biochemical and biological characteristics. Genotypic analysis using randomly amplified polymorphic DNA and simple sequence repeat-anchored polymerase chain reaction amplified profiles showed a large, genetically well-correlated group that contained the majority of the strains and a divergent group that included the PR-150 strain. For glycoconjugate composition, the PR-150 strain was different from the other strains considering the absence or presence of specific bands in aqueous or detergent phases. This strain was also totally different from the others in one out of the six parameters related to in vitro and in vivo biological behavior. We highlight the fact that the PR-150 was totally resistant to benznidazole. For the other biological parameters this strain was not totally distinct from the others, but it showed a peculiar behavior. |
description |
Thirty-two Trypanosoma cruzi strains, isolated from chronic chagasic patients in the northwest of the state of Paraná (Brazil), were analyzed using molecular, biochemical and biological characteristics. Genotypic analysis using randomly amplified polymorphic DNA and simple sequence repeat-anchored polymerase chain reaction amplified profiles showed a large, genetically well-correlated group that contained the majority of the strains and a divergent group that included the PR-150 strain. For glycoconjugate composition, the PR-150 strain was different from the other strains considering the absence or presence of specific bands in aqueous or detergent phases. This strain was also totally different from the others in one out of the six parameters related to in vitro and in vivo biological behavior. We highlight the fact that the PR-150 was totally resistant to benznidazole. For the other biological parameters this strain was not totally distinct from the others, but it showed a peculiar behavior. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500011 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762003000500011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.98 n.5 2003 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
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1669937689339625472 |