Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000600718 |
Resumo: | Tuberculosis (TB) is an infectocontagious respiratory disease caused by members of the Mycobacterium tuberculosis complex. A 7 base pair (bp) deletion in the locus polyketide synthase (pks)15/1 is described as polymorphic among members of the M. tuberculosis complex, enabling the identification of Euro-American, Indo-Oceanic and Asian lineages. The aim of this study was to characterise this locus in TB isolates from Mexico. One hundred twenty clinical isolates were recovered from the states of Veracruz and Estado de Mexico. We determined the nucleotide sequence of a ± 400 bp fragment of the locus pks15/1, while genotypic characterisation was performed by spoligotyping. One hundred and fifty isolates contained the 7 bp deletion, while five had the wild type locus. Lineages X (22%), LAM (18%) and T (17%) were the most frequent; only three (2%) of the isolates were identified as Beijing and two (1%) EAI-Manila. The wild type pks15/1 locus was observed in all Asian lineage isolates tested. Our results confirm the utility of locus pks15/1 as a molecular marker for identifying Asian lineages of the M. tuberculosis complex. This marker could be of great value in the epidemiological surveillance of TB, especially in countries like Mexico, where the prevalence of such lineages is unknown. |
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Memórias do Instituto Oswaldo Cruz |
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Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from MexicotuberculosisW-Beijingpks15/1MexicoTuberculosis (TB) is an infectocontagious respiratory disease caused by members of the Mycobacterium tuberculosis complex. A 7 base pair (bp) deletion in the locus polyketide synthase (pks)15/1 is described as polymorphic among members of the M. tuberculosis complex, enabling the identification of Euro-American, Indo-Oceanic and Asian lineages. The aim of this study was to characterise this locus in TB isolates from Mexico. One hundred twenty clinical isolates were recovered from the states of Veracruz and Estado de Mexico. We determined the nucleotide sequence of a ± 400 bp fragment of the locus pks15/1, while genotypic characterisation was performed by spoligotyping. One hundred and fifty isolates contained the 7 bp deletion, while five had the wild type locus. Lineages X (22%), LAM (18%) and T (17%) were the most frequent; only three (2%) of the isolates were identified as Beijing and two (1%) EAI-Manila. The wild type pks15/1 locus was observed in all Asian lineage isolates tested. Our results confirm the utility of locus pks15/1 as a molecular marker for identifying Asian lineages of the M. tuberculosis complex. This marker could be of great value in the epidemiological surveillance of TB, especially in countries like Mexico, where the prevalence of such lineages is unknown.Instituto Oswaldo Cruz, Ministério da Saúde2013-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000600718Memórias do Instituto Oswaldo Cruz v.108 n.6 2013reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-0276108062013007info:eu-repo/semantics/openAccessZenteno-Cuevas,RobertoSilva-Hernandez,Francisco XMendoza-Damian,FabiolaRamirez-Hernandez,Maria DoloresVazquez-Medina,KarenWidrobo-Garcia,LorenaCuellar-Sanchez,AremyMuniz-Salazar,RaquelEnciso-Moreno,LeonorPerez-Navarro,Lucia MonserratEnciso-Moreno,Jose Antonioeng2020-04-25T17:51:34Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:19:09.831Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico |
title |
Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico |
spellingShingle |
Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico Zenteno-Cuevas,Roberto tuberculosis W-Beijing pks15/1 Mexico |
title_short |
Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico |
title_full |
Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico |
title_fullStr |
Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico |
title_full_unstemmed |
Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico |
title_sort |
Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico |
author |
Zenteno-Cuevas,Roberto |
author_facet |
Zenteno-Cuevas,Roberto Silva-Hernandez,Francisco X Mendoza-Damian,Fabiola Ramirez-Hernandez,Maria Dolores Vazquez-Medina,Karen Widrobo-Garcia,Lorena Cuellar-Sanchez,Aremy Muniz-Salazar,Raquel Enciso-Moreno,Leonor Perez-Navarro,Lucia Monserrat Enciso-Moreno,Jose Antonio |
author_role |
author |
author2 |
Silva-Hernandez,Francisco X Mendoza-Damian,Fabiola Ramirez-Hernandez,Maria Dolores Vazquez-Medina,Karen Widrobo-Garcia,Lorena Cuellar-Sanchez,Aremy Muniz-Salazar,Raquel Enciso-Moreno,Leonor Perez-Navarro,Lucia Monserrat Enciso-Moreno,Jose Antonio |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Zenteno-Cuevas,Roberto Silva-Hernandez,Francisco X Mendoza-Damian,Fabiola Ramirez-Hernandez,Maria Dolores Vazquez-Medina,Karen Widrobo-Garcia,Lorena Cuellar-Sanchez,Aremy Muniz-Salazar,Raquel Enciso-Moreno,Leonor Perez-Navarro,Lucia Monserrat Enciso-Moreno,Jose Antonio |
dc.subject.por.fl_str_mv |
tuberculosis W-Beijing pks15/1 Mexico |
topic |
tuberculosis W-Beijing pks15/1 Mexico |
dc.description.none.fl_txt_mv |
Tuberculosis (TB) is an infectocontagious respiratory disease caused by members of the Mycobacterium tuberculosis complex. A 7 base pair (bp) deletion in the locus polyketide synthase (pks)15/1 is described as polymorphic among members of the M. tuberculosis complex, enabling the identification of Euro-American, Indo-Oceanic and Asian lineages. The aim of this study was to characterise this locus in TB isolates from Mexico. One hundred twenty clinical isolates were recovered from the states of Veracruz and Estado de Mexico. We determined the nucleotide sequence of a ± 400 bp fragment of the locus pks15/1, while genotypic characterisation was performed by spoligotyping. One hundred and fifty isolates contained the 7 bp deletion, while five had the wild type locus. Lineages X (22%), LAM (18%) and T (17%) were the most frequent; only three (2%) of the isolates were identified as Beijing and two (1%) EAI-Manila. The wild type pks15/1 locus was observed in all Asian lineage isolates tested. Our results confirm the utility of locus pks15/1 as a molecular marker for identifying Asian lineages of the M. tuberculosis complex. This marker could be of great value in the epidemiological surveillance of TB, especially in countries like Mexico, where the prevalence of such lineages is unknown. |
description |
Tuberculosis (TB) is an infectocontagious respiratory disease caused by members of the Mycobacterium tuberculosis complex. A 7 base pair (bp) deletion in the locus polyketide synthase (pks)15/1 is described as polymorphic among members of the M. tuberculosis complex, enabling the identification of Euro-American, Indo-Oceanic and Asian lineages. The aim of this study was to characterise this locus in TB isolates from Mexico. One hundred twenty clinical isolates were recovered from the states of Veracruz and Estado de Mexico. We determined the nucleotide sequence of a ± 400 bp fragment of the locus pks15/1, while genotypic characterisation was performed by spoligotyping. One hundred and fifty isolates contained the 7 bp deletion, while five had the wild type locus. Lineages X (22%), LAM (18%) and T (17%) were the most frequent; only three (2%) of the isolates were identified as Beijing and two (1%) EAI-Manila. The wild type pks15/1 locus was observed in all Asian lineage isolates tested. Our results confirm the utility of locus pks15/1 as a molecular marker for identifying Asian lineages of the M. tuberculosis complex. This marker could be of great value in the epidemiological surveillance of TB, especially in countries like Mexico, where the prevalence of such lineages is unknown. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000600718 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000600718 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0074-0276108062013007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.108 n.6 2013 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
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Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
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