In vitro evaluation of the effects of risedronate associated with cobalamin on osteoblastic cells
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | RGO - Revista Gaúcha de Odontologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372015000200161 |
Resumo: | OBJECTIVE: To evaluate the action of risedronate and cobalamin, and effects when associated, when administered osteoblastic cells. METHODS: The MC3T3 cells were cultivate in the media α-MEM and α-MEM supplemented with mineralizing factors, ascorbic acid and disodium α-glyicerophosphate, and treated with risedronate, cobalamin, and risedronate associated with cobalamin in a concentration of 10-3 M. The cell proliferation and formation of calcium and phosphate nodules were evaluated at 24 hours, 48 hours, 72 hours, 5 days and 7 days via a Neubauer Chamer count, Alizarin and the von Kossa reaction. RESULTS: The results showed that the growth curve for cell proliferation and the formation of mineral nodules was similar for both cultures analyzed. CONCLUSION: The conclusion was reached that using risedronate, cobalamin and both drugs in combination on osteoblastic cell cultures does not cause alterations to their growth or in the formation of calcium and phosphate nodules. |
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RGO - Revista Gaúcha de Odontologia (Online) |
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In vitro evaluation of the effects of risedronate associated with cobalamin on osteoblastic cellsVitamin B 12OsteoblastsOsteoporosis OBJECTIVE: To evaluate the action of risedronate and cobalamin, and effects when associated, when administered osteoblastic cells. METHODS: The MC3T3 cells were cultivate in the media α-MEM and α-MEM supplemented with mineralizing factors, ascorbic acid and disodium α-glyicerophosphate, and treated with risedronate, cobalamin, and risedronate associated with cobalamin in a concentration of 10-3 M. The cell proliferation and formation of calcium and phosphate nodules were evaluated at 24 hours, 48 hours, 72 hours, 5 days and 7 days via a Neubauer Chamer count, Alizarin and the von Kossa reaction. RESULTS: The results showed that the growth curve for cell proliferation and the formation of mineral nodules was similar for both cultures analyzed. CONCLUSION: The conclusion was reached that using risedronate, cobalamin and both drugs in combination on osteoblastic cell cultures does not cause alterations to their growth or in the formation of calcium and phosphate nodules.Faculdade São Leopoldo Mandic2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372015000200161RGO - Revista Gaúcha de Odontologia v.63 n.2 2015reponame:RGO - Revista Gaúcha de Odontologia (Online)instname:Faculdade São Leopoldo Mandic (FSLM)instacron:FSLM10.1590/1981-863720150002000042445info:eu-repo/semantics/openAccessFIUZA,Jaqueline AparecidaMARTINEZ,Elizabeth FerreiraBRITO JÚNIOR,Rui Barbosa deeng2019-08-06T00:00:00Zoai:scielo:S1981-86372015000200161Revistahttp://revodonto.bvsalud.org/scielo.php?script=sci_serial&pid=1981-8637&lng=pt&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||contato@revistargo.com.br1981-86370103-6971opendoar:2019-08-06T00:00RGO - Revista Gaúcha de Odontologia (Online) - Faculdade São Leopoldo Mandic (FSLM)false |
dc.title.none.fl_str_mv |
In vitro evaluation of the effects of risedronate associated with cobalamin on osteoblastic cells |
title |
In vitro evaluation of the effects of risedronate associated with cobalamin on osteoblastic cells |
spellingShingle |
In vitro evaluation of the effects of risedronate associated with cobalamin on osteoblastic cells FIUZA,Jaqueline Aparecida Vitamin B 12 Osteoblasts Osteoporosis |
title_short |
In vitro evaluation of the effects of risedronate associated with cobalamin on osteoblastic cells |
title_full |
In vitro evaluation of the effects of risedronate associated with cobalamin on osteoblastic cells |
title_fullStr |
In vitro evaluation of the effects of risedronate associated with cobalamin on osteoblastic cells |
title_full_unstemmed |
In vitro evaluation of the effects of risedronate associated with cobalamin on osteoblastic cells |
title_sort |
In vitro evaluation of the effects of risedronate associated with cobalamin on osteoblastic cells |
author |
FIUZA,Jaqueline Aparecida |
author_facet |
FIUZA,Jaqueline Aparecida MARTINEZ,Elizabeth Ferreira BRITO JÚNIOR,Rui Barbosa de |
author_role |
author |
author2 |
MARTINEZ,Elizabeth Ferreira BRITO JÚNIOR,Rui Barbosa de |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
FIUZA,Jaqueline Aparecida MARTINEZ,Elizabeth Ferreira BRITO JÚNIOR,Rui Barbosa de |
dc.subject.por.fl_str_mv |
Vitamin B 12 Osteoblasts Osteoporosis |
topic |
Vitamin B 12 Osteoblasts Osteoporosis |
description |
OBJECTIVE: To evaluate the action of risedronate and cobalamin, and effects when associated, when administered osteoblastic cells. METHODS: The MC3T3 cells were cultivate in the media α-MEM and α-MEM supplemented with mineralizing factors, ascorbic acid and disodium α-glyicerophosphate, and treated with risedronate, cobalamin, and risedronate associated with cobalamin in a concentration of 10-3 M. The cell proliferation and formation of calcium and phosphate nodules were evaluated at 24 hours, 48 hours, 72 hours, 5 days and 7 days via a Neubauer Chamer count, Alizarin and the von Kossa reaction. RESULTS: The results showed that the growth curve for cell proliferation and the formation of mineral nodules was similar for both cultures analyzed. CONCLUSION: The conclusion was reached that using risedronate, cobalamin and both drugs in combination on osteoblastic cell cultures does not cause alterations to their growth or in the formation of calcium and phosphate nodules. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372015000200161 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372015000200161 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1981-863720150002000042445 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade São Leopoldo Mandic |
publisher.none.fl_str_mv |
Faculdade São Leopoldo Mandic |
dc.source.none.fl_str_mv |
RGO - Revista Gaúcha de Odontologia v.63 n.2 2015 reponame:RGO - Revista Gaúcha de Odontologia (Online) instname:Faculdade São Leopoldo Mandic (FSLM) instacron:FSLM |
instname_str |
Faculdade São Leopoldo Mandic (FSLM) |
instacron_str |
FSLM |
institution |
FSLM |
reponame_str |
RGO - Revista Gaúcha de Odontologia (Online) |
collection |
RGO - Revista Gaúcha de Odontologia (Online) |
repository.name.fl_str_mv |
RGO - Revista Gaúcha de Odontologia (Online) - Faculdade São Leopoldo Mandic (FSLM) |
repository.mail.fl_str_mv |
||contato@revistargo.com.br |
_version_ |
1754204121208979456 |