Experimental Design as a Tool for Optimization of C-Phycocyanin Purification by Precipitation from Spirulina platensis

• Autor(a) principal: Silva, Lorena Aguiar da
• Data de Publicação: 2009
• Outros Autores: Kuhn, Kátia Regina, Moraes, Caroline Costa, Burkert, Carlos André Veiga, Kalil, Susana Juliano
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da FURG (RI FURG)
• Texto Completo: http://repositorio.furg.br/handle/1/4224
• topic: Precipitation; Ammonium sulfate; Experimental design; C-phycocyanin

C-phycocyanin is a natural blue dye that has been used as an additive in food and can be used to produce medications. The major goal of the present study was to obtain C-phycocyanin under the best operational conditions for high C-phycocyanin recovery and purity using the precipitation technique. Crude C-phycocyanin from Spirulina platensis was used. Optimization of the purification was carried out using experimental design. The effect of ammonium sulfate concentration, volume and of pH for resuspension were evaluated. Subsequently an ammonium sulfate fractionation study was carried out using the most suitable conditions found in the experimental design. The best purification condition was ammonium sulfate fractionation at 0-20%/20-50%, in relation to a resuspension volume/initial volume of 0.52 in a 7.0 pH buffer. Under these conditions, in an one-step purification only, the purity increased 70% compared to the initial extract, with an 83.8% recovery.