Effect of deoxynivalenol and T-2 toxin in malt amylase activity

• Autor(a) principal: Buffon, Jaqueline Garda
• Data de Publicação: 2010
• Outros Autores: Baraj, Edlira, Furlong, Eliana Badiale
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da FURG (RI FURG)
• Texto Completo: http://repositorio.furg.br/handle/1/1852; http://dx.doi.org/10.1590/S1516-89132010000300002
• topic: Aminolitic enzymes; Fermentation; Trichothecenes; Barley

The objective of this work was to evaluate the effect of DON and T-2 toxin levels on the malt amylase activity. The malt was contaminated in accordance with central composite design experiment with DON and T-2 toxin levels until 1000 ng/g. The activities of the enzymes were evaluated by Bernfeld method. The increase in T-2 toxin concentration inhibited the a-amylase activity. However, the increase of both toxins concentration caused inverse effect. The interaction between toxins indicated synergetic effect on the b-amylase activity. An increased activity occurred when the toxins contamination levels in malt were higher (1000ng/g malt). The trichothecenes interfered with the performance of aminolitic enzymes in the stage of malting, resulting in a significant model for enzymatic activity of b-amylase.