The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bragantia |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052004000200002 |
Resumo: | Carbon source is an indispensable factor for the development of an in vitro morphogenic program of chrysanthemum micropropagation. The choice of carbon source affects the qualitative and quantitative outcome, and also the response of thin cell layers when these are placed onto morphogenic (callus, root, shoot, somatic embryo) media. Threshold survival levels (TSLs) could not be obtained for sucrose, fructose or glucose. TSLs together with organ differentiation were, however, obtained for mannose (60-80 g.L-1), xylose and lactose (40 g.L-1) and cellulose (60-80 g.L-1), making these carbon sources suitable as potential carbon sources for positive selection systems for chrysanthemum genetic transformation. |
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The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layersDendranthema X grandifloraphytotoxicityregeneration capacitythreshold survival levelCarbon source is an indispensable factor for the development of an in vitro morphogenic program of chrysanthemum micropropagation. The choice of carbon source affects the qualitative and quantitative outcome, and also the response of thin cell layers when these are placed onto morphogenic (callus, root, shoot, somatic embryo) media. Threshold survival levels (TSLs) could not be obtained for sucrose, fructose or glucose. TSLs together with organ differentiation were, however, obtained for mannose (60-80 g.L-1), xylose and lactose (40 g.L-1) and cellulose (60-80 g.L-1), making these carbon sources suitable as potential carbon sources for positive selection systems for chrysanthemum genetic transformation.Instituto Agronômico de Campinas2004-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052004000200002Bragantia v.63 n.2 2004reponame:Bragantiainstname:Instituto Agronômico de Campinas (IAC)instacron:IAC10.1590/S0006-87052004000200002info:eu-repo/semantics/openAccessSilva,Jaime A. Teixeira daeng2004-09-15T00:00:00Zoai:scielo:S0006-87052004000200002Revistahttps://www.scielo.br/j/brag/https://old.scielo.br/oai/scielo-oai.phpbragantia@iac.sp.gov.br||bragantia@iac.sp.gov.br1678-44990006-8705opendoar:2004-09-15T00:00Bragantia - Instituto Agronômico de Campinas (IAC)false |
dc.title.none.fl_str_mv |
The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers |
title |
The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers |
spellingShingle |
The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers Silva,Jaime A. Teixeira da Dendranthema X grandiflora phytotoxicity regeneration capacity threshold survival level |
title_short |
The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers |
title_full |
The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers |
title_fullStr |
The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers |
title_full_unstemmed |
The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers |
title_sort |
The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers |
author |
Silva,Jaime A. Teixeira da |
author_facet |
Silva,Jaime A. Teixeira da |
author_role |
author |
dc.contributor.author.fl_str_mv |
Silva,Jaime A. Teixeira da |
dc.subject.por.fl_str_mv |
Dendranthema X grandiflora phytotoxicity regeneration capacity threshold survival level |
topic |
Dendranthema X grandiflora phytotoxicity regeneration capacity threshold survival level |
description |
Carbon source is an indispensable factor for the development of an in vitro morphogenic program of chrysanthemum micropropagation. The choice of carbon source affects the qualitative and quantitative outcome, and also the response of thin cell layers when these are placed onto morphogenic (callus, root, shoot, somatic embryo) media. Threshold survival levels (TSLs) could not be obtained for sucrose, fructose or glucose. TSLs together with organ differentiation were, however, obtained for mannose (60-80 g.L-1), xylose and lactose (40 g.L-1) and cellulose (60-80 g.L-1), making these carbon sources suitable as potential carbon sources for positive selection systems for chrysanthemum genetic transformation. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052004000200002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052004000200002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0006-87052004000200002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Agronômico de Campinas |
publisher.none.fl_str_mv |
Instituto Agronômico de Campinas |
dc.source.none.fl_str_mv |
Bragantia v.63 n.2 2004 reponame:Bragantia instname:Instituto Agronômico de Campinas (IAC) instacron:IAC |
instname_str |
Instituto Agronômico de Campinas (IAC) |
instacron_str |
IAC |
institution |
IAC |
reponame_str |
Bragantia |
collection |
Bragantia |
repository.name.fl_str_mv |
Bragantia - Instituto Agronômico de Campinas (IAC) |
repository.mail.fl_str_mv |
bragantia@iac.sp.gov.br||bragantia@iac.sp.gov.br |
_version_ |
1754193298263638016 |