Antioxidant activity, cytotoxic, phenolic content and phytochemical composition of Pithecellobium diversifolium Benth
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Baiana de Saúde Pública (Online) |
Texto Completo: | https://rbsp.sesab.ba.gov.br/index.php/rbsp/article/view/4018 |
Resumo: | Species of Pithecellobium (Fabaceae) are used in traditional medicine to treat diabetes, cough, bronchitis and inflammation. The present study aimed to evaluate the content and to determine the antioxidant activity, phenolic compounds content and cytotoxicity of the extract and of the fractions of Pithecellobium diversifolium, an unprecedented study with an exotic species from the Caatinga, northeastern Brazil, using HPLC-ESI instrumentation, The MeOH fractions of leaves and stem barks showed a high content of flavonoids (198.1 ± 106.50 and 542.7 ± 2.52 mg EqQ /g). The CH2Cl2 fraction of peels had a high content of total phenolic compounds (516 .7 ± 3.00 mg EqAG /g). The DPPH test showed that the CH2Cl2 fraction (leaves) had an EC50 of 0.08 ± 0.02, a value higher than that observed for the standards used in the test - Butylated hydroxyanisole (BHA), Butylated hydroxytoluene (BHT) and ascorbic acid. The AcOEt and MeOH fractions of peels had moderate cytotoxicity with values below 500 μ g/ mL. The MeOH fraction of leaves showed 7 major compounds: myricetin, quercetin, quercetin-arabinofuranoside, apigenin-triglycosides and apigenin-diglucoside, being the last three unpublished in studies involving the genus. The tests carried out show the potential of P. diversifolium, a promising source of biomolecules with therapeutic applicability. |
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Antioxidant activity, cytotoxic, phenolic content and phytochemical composition of Pithecellobium diversifolium BenthActividad antioxidante, citotóxica, contenido fenólico y composición fitoquímica de Pithecellobium diversifolium BenthAtividade antioxidante, citotóxica, conteúdo fenólico e composição fitoquímica de Pithecellobium diversifolium BenthPithecellobiumFitoquímicaPotencial antioxidanteCromatografia líquida de alta eficiênciaPithecellobiumFitoquímicaCapacidad antioxidanteCromatografía Líquida de Alta EficienciaPithecellobiumPhytochemistryAntioxidant potentialHigh performance liquid chromatographySpecies of Pithecellobium (Fabaceae) are used in traditional medicine to treat diabetes, cough, bronchitis and inflammation. The present study aimed to evaluate the content and to determine the antioxidant activity, phenolic compounds content and cytotoxicity of the extract and of the fractions of Pithecellobium diversifolium, an unprecedented study with an exotic species from the Caatinga, northeastern Brazil, using HPLC-ESI instrumentation, The MeOH fractions of leaves and stem barks showed a high content of flavonoids (198.1 ± 106.50 and 542.7 ± 2.52 mg EqQ /g). The CH2Cl2 fraction of peels had a high content of total phenolic compounds (516 .7 ± 3.00 mg EqAG /g). The DPPH test showed that the CH2Cl2 fraction (leaves) had an EC50 of 0.08 ± 0.02, a value higher than that observed for the standards used in the test - Butylated hydroxyanisole (BHA), Butylated hydroxytoluene (BHT) and ascorbic acid. The AcOEt and MeOH fractions of peels had moderate cytotoxicity with values below 500 μ g/ mL. The MeOH fraction of leaves showed 7 major compounds: myricetin, quercetin, quercetin-arabinofuranoside, apigenin-triglycosides and apigenin-diglucoside, being the last three unpublished in studies involving the genus. The tests carried out show the potential of P. diversifolium, a promising source of biomolecules with therapeutic applicability.Las especies de Pithecellobium (Fabaceae) se utilizan en medicina tradicional para tratar la diabetes, la tos, la bronquitis y la inflamación. El presente estudio tuvo como objetivo evaluar el contenido y determinar la actividad antioxidante, el contenido de compuestos fenólicos y la citotoxicidad del extracto y de las fracciones de Pithecellobium diversifolium, un estudio sin precedentes con una especie exótica de la Caatinga, noreste de Brasil, utilizando instrumentación HPLC-ESI. Las fracciones MeOH de hojas y cortezas de tallo mostraron un alto contenido de flavonoides (198,1 ± 106,50 y 542,7 ± 2,52 mg EqQ /g). La fracción CH2Cl2 de las cortezas tuvo un alto contenido de compuestos fenólicos totales (516,7 ± 3,00 mg EqAG /g). El ensayo DPPH mostró que la fracción CH2Cl2 (hojas) tenía una EC50 de 0,08 ± 0,02, un valor superior al observado para los estándares utilizados en el ensayo - butilhidroxianisol (BHA), butilhidroxitolueno (BHT) y ácido ascórbico. Las fracciones AcOEt y MeOH de las cáscaras presentaron una citotoxicidad moderada con valores inferiores a 500 μ g/ mL. La fracción MeOH de las hojas mostró 7 compuestos principales: miricetina, quercetina, quercetina-arabinofuranosido, apigenina-triglucósidos y apigenina-diglucósido, siendo los tres últimos inéditos en estudios sobre el género. Las pruebas realizadas demuestran el potencial de P. diversifolium, una fuente prometedora de biomoléculas con aplicabilidad terapéutica.Espécies de Pithecellobium (Fabaceae) são usadas na medicina tradicional para tratar diabetes, tosse, bronquite e inflamação. O presente estudo teve como objetivo avaliar o teor e determinar a atividade antioxidante, o teor de compostos fenólicos e a citotoxicidade do extrato e das frações de Pithecellobium diversifolium, um estudo inédito com uma espécie exótica da Caatinga, nordeste do Brasil, utilizando instrumentação CLAE-IES. As frações MeOH das folhas e cascas do caule apresentaram alto teor de flavonóides (198,1 ± 106,50 e 542,7 ± 2,52 mg EqQ /g). A fração CH2Cl2 das cascas apresentou um elevado teor de compostos fenólicos totais (516,7 ± 3,00 mg EqAG /g). O teste DPPH mostrou que a fracção CH2Cl2 (folhas) apresentou um EC50 de 0,08 ± 0,02, valor superior ao observado para os padrões utilizados no teste - Butil hidroxianisol (BHA), Butil hidroxitolueno (BHT) e ácido ascórbico. As fracções AcOEt e MeOH das cascas apresentaram citotoxicidade moderada com valores inferiores a 500 μg/ mL. A fração MeOH das folhas apresentou 7 compostos majoritários: miricetina, quercetina, quercetina-arabinofuranosídeo, apigenina-triglicosídeos e apigenina-diglucosídeo, sendo os três últimos inéditos em estudos envolvendo o gênero. Os testes realizados demonstram o potencial de P. diversifolium, uma promissora fonte de biomoléculas com aplicabilidade terapêutica.SESAB2024-04-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rbsp.sesab.ba.gov.br/index.php/rbsp/article/view/401810.22278/2318-2660.2024.v47.n4.a4018Revista Baiana de Saúde Pública; v. 47 n. 4 (2023); 238-2542318-26600100-023310.22278/2318-2660.2024.v47.N4reponame:Revista Baiana de Saúde Pública (Online)instname:Secretaria da Saúde do Estado da Bahia (Sesab)instacron:IBICTporhttps://rbsp.sesab.ba.gov.br/index.php/rbsp/article/view/4018/3282Copyright (c) 2024 Revista Baiana de Saúde Públicainfo:eu-repo/semantics/openAccessBarbosa da Silva, Benedita RamilaSilva, Ione Sabrina Antão da Nascimento, Amanda Dias do Teles, Pedro Henrique VieiraTavares, Josean FechineNunes, Xirley Pereira 2024-04-02T13:52:28Zoai:ojs.pkp.sfu.ca:article/4018Revistahttps://rbsp.sesab.ba.gov.br/index.php/rbspPUBhttps://rbsp.sesab.ba.gov.br/index.php/rbsp/oai||saude.revista@saude.ba.gov.br|| rbsp.saude@saude.ba.gov.br2318-26600100-0233opendoar:2024-04-02T13:52:28Revista Baiana de Saúde Pública (Online) - Secretaria da Saúde do Estado da Bahia (Sesab)false |
dc.title.none.fl_str_mv |
Antioxidant activity, cytotoxic, phenolic content and phytochemical composition of Pithecellobium diversifolium Benth Actividad antioxidante, citotóxica, contenido fenólico y composición fitoquímica de Pithecellobium diversifolium Benth Atividade antioxidante, citotóxica, conteúdo fenólico e composição fitoquímica de Pithecellobium diversifolium Benth |
title |
Antioxidant activity, cytotoxic, phenolic content and phytochemical composition of Pithecellobium diversifolium Benth |
spellingShingle |
Antioxidant activity, cytotoxic, phenolic content and phytochemical composition of Pithecellobium diversifolium Benth Barbosa da Silva, Benedita Ramila Pithecellobium Fitoquímica Potencial antioxidante Cromatografia líquida de alta eficiência Pithecellobium Fitoquímica Capacidad antioxidante Cromatografía Líquida de Alta Eficiencia Pithecellobium Phytochemistry Antioxidant potential High performance liquid chromatography |
title_short |
Antioxidant activity, cytotoxic, phenolic content and phytochemical composition of Pithecellobium diversifolium Benth |
title_full |
Antioxidant activity, cytotoxic, phenolic content and phytochemical composition of Pithecellobium diversifolium Benth |
title_fullStr |
Antioxidant activity, cytotoxic, phenolic content and phytochemical composition of Pithecellobium diversifolium Benth |
title_full_unstemmed |
Antioxidant activity, cytotoxic, phenolic content and phytochemical composition of Pithecellobium diversifolium Benth |
title_sort |
Antioxidant activity, cytotoxic, phenolic content and phytochemical composition of Pithecellobium diversifolium Benth |
author |
Barbosa da Silva, Benedita Ramila |
author_facet |
Barbosa da Silva, Benedita Ramila Silva, Ione Sabrina Antão da Nascimento, Amanda Dias do Teles, Pedro Henrique Vieira Tavares, Josean Fechine Nunes, Xirley Pereira |
author_role |
author |
author2 |
Silva, Ione Sabrina Antão da Nascimento, Amanda Dias do Teles, Pedro Henrique Vieira Tavares, Josean Fechine Nunes, Xirley Pereira |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Barbosa da Silva, Benedita Ramila Silva, Ione Sabrina Antão da Nascimento, Amanda Dias do Teles, Pedro Henrique Vieira Tavares, Josean Fechine Nunes, Xirley Pereira |
dc.subject.por.fl_str_mv |
Pithecellobium Fitoquímica Potencial antioxidante Cromatografia líquida de alta eficiência Pithecellobium Fitoquímica Capacidad antioxidante Cromatografía Líquida de Alta Eficiencia Pithecellobium Phytochemistry Antioxidant potential High performance liquid chromatography |
topic |
Pithecellobium Fitoquímica Potencial antioxidante Cromatografia líquida de alta eficiência Pithecellobium Fitoquímica Capacidad antioxidante Cromatografía Líquida de Alta Eficiencia Pithecellobium Phytochemistry Antioxidant potential High performance liquid chromatography |
description |
Species of Pithecellobium (Fabaceae) are used in traditional medicine to treat diabetes, cough, bronchitis and inflammation. The present study aimed to evaluate the content and to determine the antioxidant activity, phenolic compounds content and cytotoxicity of the extract and of the fractions of Pithecellobium diversifolium, an unprecedented study with an exotic species from the Caatinga, northeastern Brazil, using HPLC-ESI instrumentation, The MeOH fractions of leaves and stem barks showed a high content of flavonoids (198.1 ± 106.50 and 542.7 ± 2.52 mg EqQ /g). The CH2Cl2 fraction of peels had a high content of total phenolic compounds (516 .7 ± 3.00 mg EqAG /g). The DPPH test showed that the CH2Cl2 fraction (leaves) had an EC50 of 0.08 ± 0.02, a value higher than that observed for the standards used in the test - Butylated hydroxyanisole (BHA), Butylated hydroxytoluene (BHT) and ascorbic acid. The AcOEt and MeOH fractions of peels had moderate cytotoxicity with values below 500 μ g/ mL. The MeOH fraction of leaves showed 7 major compounds: myricetin, quercetin, quercetin-arabinofuranoside, apigenin-triglycosides and apigenin-diglucoside, being the last three unpublished in studies involving the genus. The tests carried out show the potential of P. diversifolium, a promising source of biomolecules with therapeutic applicability. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-04-02 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbsp.sesab.ba.gov.br/index.php/rbsp/article/view/4018 10.22278/2318-2660.2024.v47.n4.a4018 |
url |
https://rbsp.sesab.ba.gov.br/index.php/rbsp/article/view/4018 |
identifier_str_mv |
10.22278/2318-2660.2024.v47.n4.a4018 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rbsp.sesab.ba.gov.br/index.php/rbsp/article/view/4018/3282 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2024 Revista Baiana de Saúde Pública info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2024 Revista Baiana de Saúde Pública |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
SESAB |
publisher.none.fl_str_mv |
SESAB |
dc.source.none.fl_str_mv |
Revista Baiana de Saúde Pública; v. 47 n. 4 (2023); 238-254 2318-2660 0100-0233 10.22278/2318-2660.2024.v47.N4 reponame:Revista Baiana de Saúde Pública (Online) instname:Secretaria da Saúde do Estado da Bahia (Sesab) instacron:IBICT |
instname_str |
Secretaria da Saúde do Estado da Bahia (Sesab) |
instacron_str |
IBICT |
institution |
IBICT |
reponame_str |
Revista Baiana de Saúde Pública (Online) |
collection |
Revista Baiana de Saúde Pública (Online) |
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Revista Baiana de Saúde Pública (Online) - Secretaria da Saúde do Estado da Bahia (Sesab) |
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