Heterogeneity determination of bovine leukemia virus genome in Santa Catarina state, Brazil
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Publication Date: | 2018 |
Other Authors: | , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Arquivos do instituto biológico (Online) |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100211 |
Summary: | ABSTRACT: Bovine leukemia virus (BLV) is a member of Retroviridae family, genus Deltaretrovirus, and the main viral agent responsible for economic loses in dairy herds. Some studies have been carried out about BLV genotypes, and at least seven genotypes were found out in samples of different regions of the world. The objective of this study was to identify BLV samples from seropositive dairy cattle in Santa Catarina state, Brazil, using molecular techniques. Blood samples were collected (454) from dairy cattle from 31 different farms, and serology using agar gel immunodiffusion test (AGID) was performed. After that, 191 seropositive samples were submitted to DNA extraction, and in 77 samples the polymerase chain reaction (PCR) for amplification of a 440 bp fragment of the env gene was performed. Nineteen DNA samples were subjected to restriction fragment length polymorphism (RFLP) analysis by digestion of the PCR fragment by five restriction endonucleases - BamHI, HaeIII, Tru9I, TaqI, and MwoI. It was found 42% seropositive animals (191/454) and 68% positives of the farms (21/31). The PCR showed 80.5% (62/77) of animals positive. The RFLP analysis identified five different genotypes dispersed by Santa Catarina state, with the highest prevalence for genotype X (47.4%). Overall, our results identified the viral genotypes present in dairy cattle and the prevalence of new variants in representative farms from Santa Catarina state. |
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Heterogeneity determination of bovine leukemia virus genome in Santa Catarina state, BrazilBovine leukemia virusDeltaretrovirusdairy cattleenvrestriction fragment length polymorphismABSTRACT: Bovine leukemia virus (BLV) is a member of Retroviridae family, genus Deltaretrovirus, and the main viral agent responsible for economic loses in dairy herds. Some studies have been carried out about BLV genotypes, and at least seven genotypes were found out in samples of different regions of the world. The objective of this study was to identify BLV samples from seropositive dairy cattle in Santa Catarina state, Brazil, using molecular techniques. Blood samples were collected (454) from dairy cattle from 31 different farms, and serology using agar gel immunodiffusion test (AGID) was performed. After that, 191 seropositive samples were submitted to DNA extraction, and in 77 samples the polymerase chain reaction (PCR) for amplification of a 440 bp fragment of the env gene was performed. Nineteen DNA samples were subjected to restriction fragment length polymorphism (RFLP) analysis by digestion of the PCR fragment by five restriction endonucleases - BamHI, HaeIII, Tru9I, TaqI, and MwoI. It was found 42% seropositive animals (191/454) and 68% positives of the farms (21/31). The PCR showed 80.5% (62/77) of animals positive. The RFLP analysis identified five different genotypes dispersed by Santa Catarina state, with the highest prevalence for genotype X (47.4%). Overall, our results identified the viral genotypes present in dairy cattle and the prevalence of new variants in representative farms from Santa Catarina state.Instituto Biológico2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100211Arquivos do Instituto Biológico v.85 2018reponame:Arquivos do instituto biológico (Online)instname:Instituto Biológico (IB)instacron:IBIO10.1590/1808-1657000742016info:eu-repo/semantics/openAccessRodakiewicz,Sheyla MicheleFernandez,Maria LuizaMunhoz,Maria LuizaYamakawa,Flávia Harumi SchefferUrio,MonicaForell,FabianaFerraz,SandraPortes,Vagner MirandaCosta,Ubirajara Maciel daeng2018-08-21T00:00:00Zoai:scielo:S1808-16572018000100211Revistahttp://www.biologico.sp.gov.br/arquivos_bio.phphttps://old.scielo.br/oai/scielo-oai.php||arquivos@biologico.sp.gov.br1808-16570020-3653opendoar:2018-08-21T00:00Arquivos do instituto biológico (Online) - Instituto Biológico (IB)false |
dc.title.none.fl_str_mv |
Heterogeneity determination of bovine leukemia virus genome in Santa Catarina state, Brazil |
title |
Heterogeneity determination of bovine leukemia virus genome in Santa Catarina state, Brazil |
spellingShingle |
Heterogeneity determination of bovine leukemia virus genome in Santa Catarina state, Brazil Rodakiewicz,Sheyla Michele Bovine leukemia virus Deltaretrovirus dairy cattle env restriction fragment length polymorphism |
title_short |
Heterogeneity determination of bovine leukemia virus genome in Santa Catarina state, Brazil |
title_full |
Heterogeneity determination of bovine leukemia virus genome in Santa Catarina state, Brazil |
title_fullStr |
Heterogeneity determination of bovine leukemia virus genome in Santa Catarina state, Brazil |
title_full_unstemmed |
Heterogeneity determination of bovine leukemia virus genome in Santa Catarina state, Brazil |
title_sort |
Heterogeneity determination of bovine leukemia virus genome in Santa Catarina state, Brazil |
author |
Rodakiewicz,Sheyla Michele |
author_facet |
Rodakiewicz,Sheyla Michele Fernandez,Maria Luiza Munhoz,Maria Luiza Yamakawa,Flávia Harumi Scheffer Urio,Monica Forell,Fabiana Ferraz,Sandra Portes,Vagner Miranda Costa,Ubirajara Maciel da |
author_role |
author |
author2 |
Fernandez,Maria Luiza Munhoz,Maria Luiza Yamakawa,Flávia Harumi Scheffer Urio,Monica Forell,Fabiana Ferraz,Sandra Portes,Vagner Miranda Costa,Ubirajara Maciel da |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rodakiewicz,Sheyla Michele Fernandez,Maria Luiza Munhoz,Maria Luiza Yamakawa,Flávia Harumi Scheffer Urio,Monica Forell,Fabiana Ferraz,Sandra Portes,Vagner Miranda Costa,Ubirajara Maciel da |
dc.subject.por.fl_str_mv |
Bovine leukemia virus Deltaretrovirus dairy cattle env restriction fragment length polymorphism |
topic |
Bovine leukemia virus Deltaretrovirus dairy cattle env restriction fragment length polymorphism |
description |
ABSTRACT: Bovine leukemia virus (BLV) is a member of Retroviridae family, genus Deltaretrovirus, and the main viral agent responsible for economic loses in dairy herds. Some studies have been carried out about BLV genotypes, and at least seven genotypes were found out in samples of different regions of the world. The objective of this study was to identify BLV samples from seropositive dairy cattle in Santa Catarina state, Brazil, using molecular techniques. Blood samples were collected (454) from dairy cattle from 31 different farms, and serology using agar gel immunodiffusion test (AGID) was performed. After that, 191 seropositive samples were submitted to DNA extraction, and in 77 samples the polymerase chain reaction (PCR) for amplification of a 440 bp fragment of the env gene was performed. Nineteen DNA samples were subjected to restriction fragment length polymorphism (RFLP) analysis by digestion of the PCR fragment by five restriction endonucleases - BamHI, HaeIII, Tru9I, TaqI, and MwoI. It was found 42% seropositive animals (191/454) and 68% positives of the farms (21/31). The PCR showed 80.5% (62/77) of animals positive. The RFLP analysis identified five different genotypes dispersed by Santa Catarina state, with the highest prevalence for genotype X (47.4%). Overall, our results identified the viral genotypes present in dairy cattle and the prevalence of new variants in representative farms from Santa Catarina state. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100211 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100211 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1808-1657000742016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Biológico |
publisher.none.fl_str_mv |
Instituto Biológico |
dc.source.none.fl_str_mv |
Arquivos do Instituto Biológico v.85 2018 reponame:Arquivos do instituto biológico (Online) instname:Instituto Biológico (IB) instacron:IBIO |
instname_str |
Instituto Biológico (IB) |
instacron_str |
IBIO |
institution |
IBIO |
reponame_str |
Arquivos do instituto biológico (Online) |
collection |
Arquivos do instituto biológico (Online) |
repository.name.fl_str_mv |
Arquivos do instituto biológico (Online) - Instituto Biológico (IB) |
repository.mail.fl_str_mv |
||arquivos@biologico.sp.gov.br |
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1754193669998510080 |