Assessment of the oxidative metabolism of calves’ bronchoalveolar cells: comparison between NBT and fluorimetric techniques
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivos do instituto biológico (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100235 |
Resumo: | ABSTRACT: Evaluation of alveolar macrophage functions of cattle is an important tool in order to assess whether measures taken during the cattle husbandry can decrease the respiratory tract defense. The aim of this study was to determine whether dexamethasone used at therapeutic dose can affect the oxidative metabolism of alveolar macrophages of cattle. This was evaluated by two tests, the fluorometric and colorimetric. The similarity of the results was studied, using alveolar macrophages of six healthy cattle, obtained from bronchoalveolar lavage on a basal and an immunosuppressant moment after the application of dexamethasone. For the fluorometric test, alveolar macrophages were incubated with Staphylococcus aureus and 2’-7’dichlorohidroflurescein, and analyzed by flow cytometer. For the colorimetric test, alveolar macrophages were incubated with Phorbol 12- miristate-13 acetate and nitroblue tetrazolium, dissolved and analyzed in a spectrophotometer. It was noted that dexamethasone therapeutic dose (0.05 mg/kg) reduced the functions of alveolar macrophages from healthy bovine. This result was observed by both tests with the difference that the flow cytometry assay was more informative for identifying which specific cellular function has been compromised. |
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Assessment of the oxidative metabolism of calves’ bronchoalveolar cells: comparison between NBT and fluorimetric techniquesreactive oxygen speciesimmunityalveolar macrophageABSTRACT: Evaluation of alveolar macrophage functions of cattle is an important tool in order to assess whether measures taken during the cattle husbandry can decrease the respiratory tract defense. The aim of this study was to determine whether dexamethasone used at therapeutic dose can affect the oxidative metabolism of alveolar macrophages of cattle. This was evaluated by two tests, the fluorometric and colorimetric. The similarity of the results was studied, using alveolar macrophages of six healthy cattle, obtained from bronchoalveolar lavage on a basal and an immunosuppressant moment after the application of dexamethasone. For the fluorometric test, alveolar macrophages were incubated with Staphylococcus aureus and 2’-7’dichlorohidroflurescein, and analyzed by flow cytometer. For the colorimetric test, alveolar macrophages were incubated with Phorbol 12- miristate-13 acetate and nitroblue tetrazolium, dissolved and analyzed in a spectrophotometer. It was noted that dexamethasone therapeutic dose (0.05 mg/kg) reduced the functions of alveolar macrophages from healthy bovine. This result was observed by both tests with the difference that the flow cytometry assay was more informative for identifying which specific cellular function has been compromised.Instituto Biológico2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100235Arquivos do Instituto Biológico v.85 2018reponame:Arquivos do instituto biológico (Online)instname:Instituto Biológico (IB)instacron:IBIO10.1590/1808-1657000972018info:eu-repo/semantics/openAccessArtner,BrunaReck,AngelaCoelho,Alessandra MayerPrediger,Julio JoséPontarolo,Desiree VeraBertagnon,Heloisa Godoieng2018-11-07T00:00:00Zoai:scielo:S1808-16572018000100235Revistahttp://www.biologico.sp.gov.br/arquivos_bio.phphttps://old.scielo.br/oai/scielo-oai.php||arquivos@biologico.sp.gov.br1808-16570020-3653opendoar:2018-11-07T00:00Arquivos do instituto biológico (Online) - Instituto Biológico (IB)false |
dc.title.none.fl_str_mv |
Assessment of the oxidative metabolism of calves’ bronchoalveolar cells: comparison between NBT and fluorimetric techniques |
title |
Assessment of the oxidative metabolism of calves’ bronchoalveolar cells: comparison between NBT and fluorimetric techniques |
spellingShingle |
Assessment of the oxidative metabolism of calves’ bronchoalveolar cells: comparison between NBT and fluorimetric techniques Artner,Bruna reactive oxygen species immunity alveolar macrophage |
title_short |
Assessment of the oxidative metabolism of calves’ bronchoalveolar cells: comparison between NBT and fluorimetric techniques |
title_full |
Assessment of the oxidative metabolism of calves’ bronchoalveolar cells: comparison between NBT and fluorimetric techniques |
title_fullStr |
Assessment of the oxidative metabolism of calves’ bronchoalveolar cells: comparison between NBT and fluorimetric techniques |
title_full_unstemmed |
Assessment of the oxidative metabolism of calves’ bronchoalveolar cells: comparison between NBT and fluorimetric techniques |
title_sort |
Assessment of the oxidative metabolism of calves’ bronchoalveolar cells: comparison between NBT and fluorimetric techniques |
author |
Artner,Bruna |
author_facet |
Artner,Bruna Reck,Angela Coelho,Alessandra Mayer Prediger,Julio José Pontarolo,Desiree Vera Bertagnon,Heloisa Godoi |
author_role |
author |
author2 |
Reck,Angela Coelho,Alessandra Mayer Prediger,Julio José Pontarolo,Desiree Vera Bertagnon,Heloisa Godoi |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Artner,Bruna Reck,Angela Coelho,Alessandra Mayer Prediger,Julio José Pontarolo,Desiree Vera Bertagnon,Heloisa Godoi |
dc.subject.por.fl_str_mv |
reactive oxygen species immunity alveolar macrophage |
topic |
reactive oxygen species immunity alveolar macrophage |
description |
ABSTRACT: Evaluation of alveolar macrophage functions of cattle is an important tool in order to assess whether measures taken during the cattle husbandry can decrease the respiratory tract defense. The aim of this study was to determine whether dexamethasone used at therapeutic dose can affect the oxidative metabolism of alveolar macrophages of cattle. This was evaluated by two tests, the fluorometric and colorimetric. The similarity of the results was studied, using alveolar macrophages of six healthy cattle, obtained from bronchoalveolar lavage on a basal and an immunosuppressant moment after the application of dexamethasone. For the fluorometric test, alveolar macrophages were incubated with Staphylococcus aureus and 2’-7’dichlorohidroflurescein, and analyzed by flow cytometer. For the colorimetric test, alveolar macrophages were incubated with Phorbol 12- miristate-13 acetate and nitroblue tetrazolium, dissolved and analyzed in a spectrophotometer. It was noted that dexamethasone therapeutic dose (0.05 mg/kg) reduced the functions of alveolar macrophages from healthy bovine. This result was observed by both tests with the difference that the flow cytometry assay was more informative for identifying which specific cellular function has been compromised. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100235 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100235 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1808-1657000972018 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Biológico |
publisher.none.fl_str_mv |
Instituto Biológico |
dc.source.none.fl_str_mv |
Arquivos do Instituto Biológico v.85 2018 reponame:Arquivos do instituto biológico (Online) instname:Instituto Biológico (IB) instacron:IBIO |
instname_str |
Instituto Biológico (IB) |
instacron_str |
IBIO |
institution |
IBIO |
reponame_str |
Arquivos do instituto biológico (Online) |
collection |
Arquivos do instituto biológico (Online) |
repository.name.fl_str_mv |
Arquivos do instituto biológico (Online) - Instituto Biológico (IB) |
repository.mail.fl_str_mv |
||arquivos@biologico.sp.gov.br |
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1754193670318325760 |