Mebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01)
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3794 |
Resumo: | The objective of study was to examine the role of MBZ on malignant ascites cells and the involvement of C-MYC. Comet assay was used to assess the genotoxic effects of MBZ in AGP01 cells and human lymphocytes; differential staining by ethidium bromide and acridine orange, caspase 3/7 and flow cytometry assay was done to access the mechanisms of apoptosis and cell cycle analysis of MBZ in AGP01 cells. C-MYC amplification, C-MYC mRNA and C-MYC protein expression were evaluated by FISH, RT-qPCR and Western blotting, respectively. In addition, cytotoxicity of MBZ was evaluated in AGP01 and AGP01 shRNA MYC by MTT. MBZ significantly increased the damage index and no produced in human lymphocytes. MBZ caused remarkable cell cycle arrest in G0/G1 and G2/M phases at 0.5μM and 1.0 μM, respectively and induced significantly apoptosis in higher concentrations. Additionally, MBZ (0.5 μM and 1.0 μM) increased caspase 3 and 7 activities. MBZ decreased signals, C-MYC mRNA and C-MYC protein expression in AGP01 cells. MBZ induced lower cell viability in AGP01 cells compared AGP01 shRNA MYC in the same concentration. Therefore, our results show the evidence of C-MYC gene as one of the pathways by which MBZ induces cell death in gastric cancer cells. |
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Pinto, Laine CelestinoMesquita, Felipe PantojaSoares, Bruno MoreiraSilva, Emerson Lucena daPuty, BrunaOliveira, Edivaldo Herculano Correa deBurbano, Rommel RodriguezMontenegro, Raquel Carvalho2019-06-27T13:38:51Z2019-06-27T13:38:51Z2019PINTO, Laine Celestino et al. Mebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01). Toxicology in Vitro, v. 60, n. xx, p. 305-312, Oct. 2019. DOI: https://doi.org/10.1016/j.tiv.2019.06.0101879-3177https://patua.iec.gov.br/handle/iec/379410.1016/j.tiv.2019.06.010The objective of study was to examine the role of MBZ on malignant ascites cells and the involvement of C-MYC. Comet assay was used to assess the genotoxic effects of MBZ in AGP01 cells and human lymphocytes; differential staining by ethidium bromide and acridine orange, caspase 3/7 and flow cytometry assay was done to access the mechanisms of apoptosis and cell cycle analysis of MBZ in AGP01 cells. C-MYC amplification, C-MYC mRNA and C-MYC protein expression were evaluated by FISH, RT-qPCR and Western blotting, respectively. In addition, cytotoxicity of MBZ was evaluated in AGP01 and AGP01 shRNA MYC by MTT. MBZ significantly increased the damage index and no produced in human lymphocytes. MBZ caused remarkable cell cycle arrest in G0/G1 and G2/M phases at 0.5μM and 1.0 μM, respectively and induced significantly apoptosis in higher concentrations. Additionally, MBZ (0.5 μM and 1.0 μM) increased caspase 3 and 7 activities. MBZ decreased signals, C-MYC mRNA and C-MYC protein expression in AGP01 cells. MBZ induced lower cell viability in AGP01 cells compared AGP01 shRNA MYC in the same concentration. Therefore, our results show the evidence of C-MYC gene as one of the pathways by which MBZ induces cell death in gastric cancer cells.Federal University of Pará. Biological Science Institute. Laboratory of Experimental Neuropathology. Belém, PA, Brazil.Federal University of Ceará. Drug Research and Development Center. Laboratory of Pharmacogenetics. Fortaleza, CE, Brazil.Federal University of Pará. Biological Science Institute. Laboratory of Human Cytogenetics. Belém, PA, Brazil.Federal University of Ceará. Drug Research and Development Center. Laboratory of Pharmacogenetics. Fortaleza, CE, Brazil.Federal University of Pará. Laboratory of Structural and Functional Biology Science. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura Celular e Citogenética. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura Celular e Citogenética. Ananindeua, PA, Brasil.Federal University of Pará. Biological Science Institute. Laboratory of Human Cytogenetics. Belém, PA, Brazil.Federal University of Ceará. Drug Research and Development Center. Laboratory of Pharmacogenetics. Fortaleza, CE, Brazil.engElsevierMebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNeoplasias Gástricas / patologiaGenes myc / genéticaMebendazol / efeitos adversosAntinematódeosApoptoseinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALAcesso Embargado.pdfAcesso Embargado.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/5cacdb48-b183-4350-92c7-3f77fc7db83a/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/e286f686-6384-4d20-90fc-6120d1515412/download11832eea31b16df8613079d742d61793MD52TEXTMebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01).pdf.txtMebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01).pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/b53a64fe-1320-4e6b-829f-d91093e7bbdd/downloade1c06d85ae7b8b032bef47e42e4c08f9MD55Acesso Embargado.pdf.txtAcesso Embargado.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/eecd5423-2987-4b04-9e56-fc8a23c5f8b4/downloade1c06d85ae7b8b032bef47e42e4c08f9MD57THUMBNAILMebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01).pdf.jpgMebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01).pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/6a7df47c-ad55-4b37-973e-251475ce5d7b/download71859d578212107f7f8c49a4ce09d9eeMD56Acesso Embargado.pdf.jpgAcesso Embargado.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/d8d5395a-c008-47eb-adf4-f48d4e298643/download71859d578212107f7f8c49a4ce09d9eeMD58iec/37942023-06-19 13:25:50.173oai:patua.iec.gov.br:iec/3794https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-06-19T13:25:50Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv |
Mebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01) |
title |
Mebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01) |
spellingShingle |
Mebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01) Pinto, Laine Celestino Neoplasias Gástricas / patologia Genes myc / genética Mebendazol / efeitos adversos Antinematódeos Apoptose |
title_short |
Mebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01) |
title_full |
Mebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01) |
title_fullStr |
Mebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01) |
title_full_unstemmed |
Mebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01) |
title_sort |
Mebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01) |
author |
Pinto, Laine Celestino |
author_facet |
Pinto, Laine Celestino Mesquita, Felipe Pantoja Soares, Bruno Moreira Silva, Emerson Lucena da Puty, Bruna Oliveira, Edivaldo Herculano Correa de Burbano, Rommel Rodriguez Montenegro, Raquel Carvalho |
author_role |
author |
author2 |
Mesquita, Felipe Pantoja Soares, Bruno Moreira Silva, Emerson Lucena da Puty, Bruna Oliveira, Edivaldo Herculano Correa de Burbano, Rommel Rodriguez Montenegro, Raquel Carvalho |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Pinto, Laine Celestino Mesquita, Felipe Pantoja Soares, Bruno Moreira Silva, Emerson Lucena da Puty, Bruna Oliveira, Edivaldo Herculano Correa de Burbano, Rommel Rodriguez Montenegro, Raquel Carvalho |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Neoplasias Gástricas / patologia Genes myc / genética Mebendazol / efeitos adversos Antinematódeos Apoptose |
topic |
Neoplasias Gástricas / patologia Genes myc / genética Mebendazol / efeitos adversos Antinematódeos Apoptose |
description |
The objective of study was to examine the role of MBZ on malignant ascites cells and the involvement of C-MYC. Comet assay was used to assess the genotoxic effects of MBZ in AGP01 cells and human lymphocytes; differential staining by ethidium bromide and acridine orange, caspase 3/7 and flow cytometry assay was done to access the mechanisms of apoptosis and cell cycle analysis of MBZ in AGP01 cells. C-MYC amplification, C-MYC mRNA and C-MYC protein expression were evaluated by FISH, RT-qPCR and Western blotting, respectively. In addition, cytotoxicity of MBZ was evaluated in AGP01 and AGP01 shRNA MYC by MTT. MBZ significantly increased the damage index and no produced in human lymphocytes. MBZ caused remarkable cell cycle arrest in G0/G1 and G2/M phases at 0.5μM and 1.0 μM, respectively and induced significantly apoptosis in higher concentrations. Additionally, MBZ (0.5 μM and 1.0 μM) increased caspase 3 and 7 activities. MBZ decreased signals, C-MYC mRNA and C-MYC protein expression in AGP01 cells. MBZ induced lower cell viability in AGP01 cells compared AGP01 shRNA MYC in the same concentration. Therefore, our results show the evidence of C-MYC gene as one of the pathways by which MBZ induces cell death in gastric cancer cells. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-06-27T13:38:51Z |
dc.date.available.fl_str_mv |
2019-06-27T13:38:51Z |
dc.date.issued.fl_str_mv |
2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PINTO, Laine Celestino et al. Mebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01). Toxicology in Vitro, v. 60, n. xx, p. 305-312, Oct. 2019. DOI: https://doi.org/10.1016/j.tiv.2019.06.010 |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3794 |
dc.identifier.issn.-.fl_str_mv |
1879-3177 |
dc.identifier.doi.-.fl_str_mv |
10.1016/j.tiv.2019.06.010 |
identifier_str_mv |
PINTO, Laine Celestino et al. Mebendazole induces apoptosis via C-MYC inactivation in malignant ascites cell line (AGP01). Toxicology in Vitro, v. 60, n. xx, p. 305-312, Oct. 2019. DOI: https://doi.org/10.1016/j.tiv.2019.06.010 1879-3177 10.1016/j.tiv.2019.06.010 |
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https://patua.iec.gov.br/handle/iec/3794 |
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eng |
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eng |
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Elsevier |
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Elsevier |
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