Endemic high-risk clone ST277 is related to the spread of SPM-1-producing Pseudomonas aeruginosa during the COVID-19 pandemic period in northern Brazil

Detalhes bibliográficos
Autor(a) principal: Santos, Pabllo Antonny Silva dos
Data de Publicação: 2023
Outros Autores: Rodrigues, Yan Corrêa, Marcon, Davi Josué, Lobato, Amália Raiana Fonseca, Cazuza, Thalyta Braga, Gouveia, Maria Isabel Montoril, Silva, Marcos Jessé Abrahão, Souza, Alex Brito, Lima, Luana Nepomuceno Gondim Costa, Quaresma, Ana Judith Pires Garcia, Brasiliense, Danielle Murici, Lima, Karla Valéria Batista
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/6926
Resumo: Pseudomonas aeruginosa is a high-priority bacterial agent that causes healthcare-acquired infections (HAIs), which often leads to serious infections and poor prognosis in vulnerable patients. Its increasing resistance to antimicrobials, associated with SPM production, is a case of public health concern. Therefore, this study aims to determine the antimicrobial resistance, virulence, and genotyping features of P. aeruginosa strains producing SPM-1 in the Northern region of Brazil. To determine the presence of virulence and resistance genes, the PCR technique was used. For the susceptibility profile of antimicrobials, the Kirby–Bauer disk diffusion method was performed on Mueller–Hinton agar. The MLST technique was used to define the ST of the isolates. The exoS+/exoU− virulotype was standard for all strains, with the aprA, lasA, toxA, exoS, exoT, and exoY genes as the most prevalent. All the isolates showed an MDR or XDR profile against the six classes of antimicrobials tested. HRC ST277 played a major role in spreading the SPM-1-producing P. aeruginosa strains.
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spelling Santos, Pabllo Antonny Silva dosRodrigues, Yan CorrêaMarcon, Davi JosuéLobato, Amália Raiana FonsecaCazuza, Thalyta BragaGouveia, Maria Isabel MontorilSilva, Marcos Jessé AbrahãoSouza, Alex BritoLima, Luana Nepomuceno Gondim CostaQuaresma, Ana Judith Pires GarciaBrasiliense, Danielle MuriciLima, Karla Valéria Batista2023-09-05T11:49:02Z2023-09-05T11:49:02Z2023SANTOS, Pabllo Antonny Silva dos et al. Endemic high-risk clone ST277 is related to the spread of SPM-1-producing Pseudomonas aeruginosa during the COVID-19 pandemic period in northern Brazil. Microorganisms, v. 11, n. 8, p. 1-15, 2023. DOI: https://doi.org//10.3390/microorganisms11082069. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457858/pdf/microorganisms-11-02069.pdf.2076-2607https://patua.iec.gov.br/handle/iec/692610.3390/microorganisms11082069Pseudomonas aeruginosa is a high-priority bacterial agent that causes healthcare-acquired infections (HAIs), which often leads to serious infections and poor prognosis in vulnerable patients. Its increasing resistance to antimicrobials, associated with SPM production, is a case of public health concern. Therefore, this study aims to determine the antimicrobial resistance, virulence, and genotyping features of P. aeruginosa strains producing SPM-1 in the Northern region of Brazil. To determine the presence of virulence and resistance genes, the PCR technique was used. For the susceptibility profile of antimicrobials, the Kirby–Bauer disk diffusion method was performed on Mueller–Hinton agar. The MLST technique was used to define the ST of the isolates. The exoS+/exoU− virulotype was standard for all strains, with the aprA, lasA, toxA, exoS, exoT, and exoY genes as the most prevalent. All the isolates showed an MDR or XDR profile against the six classes of antimicrobials tested. HRC ST277 played a major role in spreading the SPM-1-producing P. aeruginosa strains.The APC was funded by Evandro Chaga Institute (IEC/PA). Yan Corrêa Rodrigues scholarship is funded by PDPG—Pós-Doutorado Estratégico (PDPG-POSDOC), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES/Edital 16/2022). Pabllo Antonny Silva Dos Santos is funded by CAPESState University of Pará. Program in Parasitic Biology in the Amazon Region. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Programa de Epidemiologia e Vigilância em Saúde. Ananindeua, PA, Brasil / State University of Pará. Department of Natural Science. Belém, PA, Brazil.State University of Pará. Program in Parasitic Biology in the Amazon Region. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Programa de Epidemiologia e Vigilância em Saúde. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.State University of Pará. Program in Parasitic Biology in the Amazon Region. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Programa de Epidemiologia e Vigilância em Saúde. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.State University of Pará. Program in Parasitic Biology in the Amazon Region. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Programa de Epidemiologia e Vigilância em Saúde. Ananindeua, PA, Brasil.State University of Pará. Program in Parasitic Biology in the Amazon Region. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Programa de Epidemiologia e Vigilância em Saúde. Ananindeua, PA, Brasil.engMDPIEndemic high-risk clone ST277 is related to the spread of SPM-1-producing Pseudomonas aeruginosa during the COVID-19 pandemic period in northern Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePseudomonas aeruginosa / patogenicidadeInfecções por PseudomonasCarbapenêmicosVirulênciaCOVID-19 / históriainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALEndemic high-risk clone ST277 is related to the spread of SPM-1-producing Pseudomonas aeruginosa during the COVID-19 pandemic period in northern Brazil.pdfEndemic high-risk clone ST277 is related to the spread of SPM-1-producing Pseudomonas aeruginosa during the COVID-19 pandemic period in northern Brazil.pdfapplication/pdf653386https://patua.iec.gov.br/bitstreams/6783a762-6f7a-49d8-a9dd-409f0f034fd3/download3aaeb2f5be65eaf0e13d3f630bf31329MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Endemic high-risk clone ST277 is related to the spread of SPM-1-producing Pseudomonas aeruginosa during the COVID-19 pandemic period in northern Brazil
title Endemic high-risk clone ST277 is related to the spread of SPM-1-producing Pseudomonas aeruginosa during the COVID-19 pandemic period in northern Brazil
spellingShingle Endemic high-risk clone ST277 is related to the spread of SPM-1-producing Pseudomonas aeruginosa during the COVID-19 pandemic period in northern Brazil
Santos, Pabllo Antonny Silva dos
Pseudomonas aeruginosa / patogenicidade
Infecções por Pseudomonas
Carbapenêmicos
Virulência
COVID-19 / história
title_short Endemic high-risk clone ST277 is related to the spread of SPM-1-producing Pseudomonas aeruginosa during the COVID-19 pandemic period in northern Brazil
title_full Endemic high-risk clone ST277 is related to the spread of SPM-1-producing Pseudomonas aeruginosa during the COVID-19 pandemic period in northern Brazil
title_fullStr Endemic high-risk clone ST277 is related to the spread of SPM-1-producing Pseudomonas aeruginosa during the COVID-19 pandemic period in northern Brazil
title_full_unstemmed Endemic high-risk clone ST277 is related to the spread of SPM-1-producing Pseudomonas aeruginosa during the COVID-19 pandemic period in northern Brazil
title_sort Endemic high-risk clone ST277 is related to the spread of SPM-1-producing Pseudomonas aeruginosa during the COVID-19 pandemic period in northern Brazil
author Santos, Pabllo Antonny Silva dos
author_facet Santos, Pabllo Antonny Silva dos
Rodrigues, Yan Corrêa
Marcon, Davi Josué
Lobato, Amália Raiana Fonseca
Cazuza, Thalyta Braga
Gouveia, Maria Isabel Montoril
Silva, Marcos Jessé Abrahão
Souza, Alex Brito
Lima, Luana Nepomuceno Gondim Costa
Quaresma, Ana Judith Pires Garcia
Brasiliense, Danielle Murici
Lima, Karla Valéria Batista
author_role author
author2 Rodrigues, Yan Corrêa
Marcon, Davi Josué
Lobato, Amália Raiana Fonseca
Cazuza, Thalyta Braga
Gouveia, Maria Isabel Montoril
Silva, Marcos Jessé Abrahão
Souza, Alex Brito
Lima, Luana Nepomuceno Gondim Costa
Quaresma, Ana Judith Pires Garcia
Brasiliense, Danielle Murici
Lima, Karla Valéria Batista
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Pabllo Antonny Silva dos
Rodrigues, Yan Corrêa
Marcon, Davi Josué
Lobato, Amália Raiana Fonseca
Cazuza, Thalyta Braga
Gouveia, Maria Isabel Montoril
Silva, Marcos Jessé Abrahão
Souza, Alex Brito
Lima, Luana Nepomuceno Gondim Costa
Quaresma, Ana Judith Pires Garcia
Brasiliense, Danielle Murici
Lima, Karla Valéria Batista
dc.subject.decsPrimary.pt_BR.fl_str_mv Pseudomonas aeruginosa / patogenicidade
Infecções por Pseudomonas
Carbapenêmicos
Virulência
COVID-19 / história
topic Pseudomonas aeruginosa / patogenicidade
Infecções por Pseudomonas
Carbapenêmicos
Virulência
COVID-19 / história
description Pseudomonas aeruginosa is a high-priority bacterial agent that causes healthcare-acquired infections (HAIs), which often leads to serious infections and poor prognosis in vulnerable patients. Its increasing resistance to antimicrobials, associated with SPM production, is a case of public health concern. Therefore, this study aims to determine the antimicrobial resistance, virulence, and genotyping features of P. aeruginosa strains producing SPM-1 in the Northern region of Brazil. To determine the presence of virulence and resistance genes, the PCR technique was used. For the susceptibility profile of antimicrobials, the Kirby–Bauer disk diffusion method was performed on Mueller–Hinton agar. The MLST technique was used to define the ST of the isolates. The exoS+/exoU− virulotype was standard for all strains, with the aprA, lasA, toxA, exoS, exoT, and exoY genes as the most prevalent. All the isolates showed an MDR or XDR profile against the six classes of antimicrobials tested. HRC ST277 played a major role in spreading the SPM-1-producing P. aeruginosa strains.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-09-05T11:49:02Z
dc.date.available.fl_str_mv 2023-09-05T11:49:02Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv SANTOS, Pabllo Antonny Silva dos et al. Endemic high-risk clone ST277 is related to the spread of SPM-1-producing Pseudomonas aeruginosa during the COVID-19 pandemic period in northern Brazil. Microorganisms, v. 11, n. 8, p. 1-15, 2023. DOI: https://doi.org//10.3390/microorganisms11082069. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457858/pdf/microorganisms-11-02069.pdf.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/6926
dc.identifier.issn.-.fl_str_mv 2076-2607
dc.identifier.doi.pt_BR.fl_str_mv 10.3390/microorganisms11082069
identifier_str_mv SANTOS, Pabllo Antonny Silva dos et al. Endemic high-risk clone ST277 is related to the spread of SPM-1-producing Pseudomonas aeruginosa during the COVID-19 pandemic period in northern Brazil. Microorganisms, v. 11, n. 8, p. 1-15, 2023. DOI: https://doi.org//10.3390/microorganisms11082069. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457858/pdf/microorganisms-11-02069.pdf.
2076-2607
10.3390/microorganisms11082069
url https://patua.iec.gov.br/handle/iec/6926
dc.language.iso.fl_str_mv eng
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