Further enzymic characeters of Trypanosoma cruzi and their evaluation for strain identification
Autor(a) principal: | |
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Data de Publicação: | 1980 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/807 |
Resumo: | Starch-gel electrophoresis of 38 enzymes was attempted with extracts of Trypanosoma cruzi culture forms. 18 of the enzymes that gave discrete electrophoretic bands were selected for routine characterization of T. cruzi stocks; the enzymes were: aspartate aminotransferase (E.C.2.6.1.1, ASAT); alanine aminotransferase (E.C.2.6.1.2, ALAT); phosphoglucomutase (E.C.2.7.5.1, PGM); glucosephosphate isomerase (E.C.5.3.1.9, GPI); malate dehydrogenase (oxaloacetate decarboxylating) (NADP+) (E.C.l.l.l.40, ME); glucose 6- phosphate dehydrogenase (E.C.l.l.l.49, G6PD); malate dehydrogenase (E.C.l.l.l.37, MDH); aconitate hydratase (E.C.4.2.1.3, ACON); isocitrate dehydrogenase (NADP+) (E.C.l.l.l.42, ICD); alcohol dehydrogenase (NADP+) (E.C.l.l.l.2, ADH); lactate dehydrogenase (E.C.l.l.l.27,LDH); aminopeptidase (cytosol) (E.C.3.4.11.1, PEP); pyruvate kinase (E.C.2.7.1.40, PK); phosphoglycerate kinase (E.C.2.7.2.3, PGK); enolase (E.C.4.2.1.11, ENO); hexokinase (E.C.2.7.1.1, HK); mannosephosphate isomerase (E.C.5.3.1.8, MPI); and glutamate dehydrogenase (E.C.l.4.1.2, GD). ADH (NADP+) in the genus Trypanosoma, and PGK, MPI and ENO, in T. cruzi, were apparently demonstrated for the first time. Between six and 18 enzymes were used to characterize more than 250 T. cruzi stocks, newly isolated from a wide range of sources in northern and central Brazil. Ali stocks were identified as belonging to T. cruzi zymodemes 1, 2 or 3, as originally defined-that is, by combination of electrophoretic patterns of ASAT, ALAT, PGM, GPI, ME and G6PD. The composite range of results with ali enzymes confirmed the presence of three principal T. cruzi zymodemes, but some enzymic characters overlapped between zymodemes and others suggested subgroups within individual zymodemes. Seven (MDH, ACON, LDH, PK, PGK, ENO, HK) of the 18 enzymes did not distinguish the three zymodemes; tive (ASA T, PGM, GPI, ICD, PEP) distinguished ali three zymodemes; 10 (ASAT, ALAT, PGM, GPI, ME, G6PD, ICD, ADH, PEP, GD) distinguished zymodemes 1 and 2, of which seven plus MPI and eight plus MPI separated zymodemes 1 from 3 and 2 from 3 respectively. T. cruzi stocks were taken from a smali area of the natural species distribution; the fuli range of enzymic characters within the species T. cruzi is expected to be far more complex. The epidemiological distribution of the zymodemes continued to accord with local transmission cycles and supported the hypothesis that distinct T. cruzi strains might be responsible for the enigmatic distribution of chronic Chagas's disease. Some of the difficulties in the empirical selection of new electrophoretic methods and the interpretation of results were presented, and the present and prospective significance of T. cruzi enzymic characters was discussed. Until the stability and genetic basis of T. cruzi enzymic characters are better understood it is recommended that isoenzymic profiles be confirmed routinely, botIl before and after stocks are used experimentally, as representative of a given zymodeme. A multiple biochemical approach to T. cruzi strain identification is recommended, using characters suitable for a numerical taxonomy. |
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Miles, M. ALanham, Sheila MSouza, Adelson Alcimar Almeida dePóvoa, Marinete Marins2016-01-26T11:36:54Z1980MILES, M. A. et al. Further enzymic characeters of Trypanosoma cruzi and their evaluation for strain identification. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 74, n. 2, p. 221-237, 1980.0035-9203https://patua.iec.gov.br/handle/iec/807Starch-gel electrophoresis of 38 enzymes was attempted with extracts of Trypanosoma cruzi culture forms. 18 of the enzymes that gave discrete electrophoretic bands were selected for routine characterization of T. cruzi stocks; the enzymes were: aspartate aminotransferase (E.C.2.6.1.1, ASAT); alanine aminotransferase (E.C.2.6.1.2, ALAT); phosphoglucomutase (E.C.2.7.5.1, PGM); glucosephosphate isomerase (E.C.5.3.1.9, GPI); malate dehydrogenase (oxaloacetate decarboxylating) (NADP+) (E.C.l.l.l.40, ME); glucose 6- phosphate dehydrogenase (E.C.l.l.l.49, G6PD); malate dehydrogenase (E.C.l.l.l.37, MDH); aconitate hydratase (E.C.4.2.1.3, ACON); isocitrate dehydrogenase (NADP+) (E.C.l.l.l.42, ICD); alcohol dehydrogenase (NADP+) (E.C.l.l.l.2, ADH); lactate dehydrogenase (E.C.l.l.l.27,LDH); aminopeptidase (cytosol) (E.C.3.4.11.1, PEP); pyruvate kinase (E.C.2.7.1.40, PK); phosphoglycerate kinase (E.C.2.7.2.3, PGK); enolase (E.C.4.2.1.11, ENO); hexokinase (E.C.2.7.1.1, HK); mannosephosphate isomerase (E.C.5.3.1.8, MPI); and glutamate dehydrogenase (E.C.l.4.1.2, GD). ADH (NADP+) in the genus Trypanosoma, and PGK, MPI and ENO, in T. cruzi, were apparently demonstrated for the first time. Between six and 18 enzymes were used to characterize more than 250 T. cruzi stocks, newly isolated from a wide range of sources in northern and central Brazil. Ali stocks were identified as belonging to T. cruzi zymodemes 1, 2 or 3, as originally defined-that is, by combination of electrophoretic patterns of ASAT, ALAT, PGM, GPI, ME and G6PD. The composite range of results with ali enzymes confirmed the presence of three principal T. cruzi zymodemes, but some enzymic characters overlapped between zymodemes and others suggested subgroups within individual zymodemes. Seven (MDH, ACON, LDH, PK, PGK, ENO, HK) of the 18 enzymes did not distinguish the three zymodemes; tive (ASA T, PGM, GPI, ICD, PEP) distinguished ali three zymodemes; 10 (ASAT, ALAT, PGM, GPI, ME, G6PD, ICD, ADH, PEP, GD) distinguished zymodemes 1 and 2, of which seven plus MPI and eight plus MPI separated zymodemes 1 from 3 and 2 from 3 respectively. T. cruzi stocks were taken from a smali area of the natural species distribution; the fuli range of enzymic characters within the species T. cruzi is expected to be far more complex. The epidemiological distribution of the zymodemes continued to accord with local transmission cycles and supported the hypothesis that distinct T. cruzi strains might be responsible for the enigmatic distribution of chronic Chagas's disease. Some of the difficulties in the empirical selection of new electrophoretic methods and the interpretation of results were presented, and the present and prospective significance of T. cruzi enzymic characters was discussed. Until the stability and genetic basis of T. cruzi enzymic characters are better understood it is recommended that isoenzymic profiles be confirmed routinely, botIl before and after stocks are used experimentally, as representative of a given zymodeme. A multiple biochemical approach to T. cruzi strain identification is recommended, using characters suitable for a numerical taxonomy.Electroforese em gel de amido de 38 enzimas foi tentada com extratos de formas de cultura de T. cruzi. Dezoito das enzimas, as quais deram discretas manchas electroforéticas, foram selecionadas para carcaterização de rotina dos stocks de T. cruzi; as enzimas foram asparato aminotransferase (E.C. 2.6.1.1, ASAT); alanina aminotransferase (E.C. 2.6.1.2, ALAT); fosfoglucomutase (E.C.2.7.5.1, PGM); glicosefosfato isomerase (E.C.5.3.1.9, GPI); malato dehidrogenase (oxaloacetato descarboxilando (NADP+) (E.C.l.l.l.40, ME); glicose 6-fosfato dehidrogenase (E.C.l.l.l.49, G6PD); malate dehidrogenase (E.C.l.l.l.37, MDH); aconitato hidratase (E.C.4.2.1.3, ACON); isocitrato dehidrogenase (NADP+) (E.C.l.l.l.42, ICD); alcool dehidrogenase (NADP+) (E.C.l.l.l.2, ADH); lactato dehidrogenase (E.C.l.l.l.27, LDH); aminopeptidase (citosol) (E.C.3.4.11.1, PEP); piruvato quinase (E.C.2.7.1.40, PK); fosfoglicerato quinase (E.C.2.7.2.3, PGK); enolase (E.C.4.2.1.11, ENO); hexoquinase (E.C.2.7.1.1, HK); manosefosfato isomerase (E.C.5.3.1.8, MPI); e glutamato dehidrogenase (E.C.I.4.1.2, GD). ADH (NADP+), no genero Trypanosoma e MPI, PGK e ENO, em T. cruzi, foram demonstradas pela primeira vez. Entre 6 e 18 enzimas foram usadas para caracterizar mais de 250 stocks de T. cruzi, recentemente isoladas de varias origens nas partes norte e central do Brasil. Todos os stocks foram identificados como pertencentes aos zymodemes de T. cruzi I, 2 ou 3, como originalmente definidosisto é, pela combinação de padrões electroforéticos de ASAT, ALAT, PGM, GPI, ME e G6PD. A variabilidade de resultados combinados com todas as enzimas confumaram a presença de 3 zymodemes principais de T. cruzi mas alguns caracteres enzimaticos sobrepostos entre os zymodemes, e outros sugeriram subgrupos dentro de zymodemes individuais. Sete (MDH, ACON, LDH, PK, PGK, ENO, HK) das 18 enzimas não distinguiram os 3 zymodemes; 5 (ASAT, PGM, GPI, ICD, PEP) distinguiram todos os 3 zymodemes; 10 (ASA T , ALAT, PGM, GPI, ME, G6PD, ICD, ADH, PEP, GD) distinguiram zymodemes 1 e 2; dos quais 7 mais MPI e 8 mais MPI separaram zymodemes 1 de 3 e 2 de 3 respectivamente. Stocks de T. cruzi foram trazidos de uma pequena área de distribuição natural da espécie; é considerado que a total variedade dos caracteres enzimáticos dentro das espécies de T. cruzi sera muito mais complexa. A distribuição epidemiol6gica dos zymodemes continuou em acordo com os ciclos de transmissão local e reforçou a hipotese que amostras distintas de T. cruzi poderiam ser responsaveis pela distribuição enigmática de Doença de Chagas crônica. Algumas dificuldades na seleção empirica de novos métodos eletroforéticos e a interpretação dos resultados foram descritos, e o valor, presente e prospectivo, de caracteres enzimáticos de T. cruzi foi discutido. A stabilidade e base genética de caracteres enzimáticos de T. cruzi não são completemente entendidos então é recomendade que perfis isoenzimaticos sejam confirmados rotineiramente, ambos antes e depois dos stocks serem usados experimentalmente, como representativo de um dado zymodeme. Uma multipla proximidade bioquimica para identificação de amostras de T. cruzi é recomendada, usando caracteres apropriados para uma taxonomia numérica.Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil / London School of Hygiene and Tropical Medicine. Departament of Medical Protozoology. Keppel Street, London.London School of Hygiene and Tropical Medicine. Departament of Medical Protozoology. Keppel Street, London.Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, BrasilMinistério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. 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dc.title.pt_BR.fl_str_mv |
Further enzymic characeters of Trypanosoma cruzi and their evaluation for strain identification |
title |
Further enzymic characeters of Trypanosoma cruzi and their evaluation for strain identification |
spellingShingle |
Further enzymic characeters of Trypanosoma cruzi and their evaluation for strain identification Miles, M. A Trypanosoma cruzi / classificação Trypanosoma cruzi / enzimologia Trypanosoma cruzi / isolamento & purificação |
title_short |
Further enzymic characeters of Trypanosoma cruzi and their evaluation for strain identification |
title_full |
Further enzymic characeters of Trypanosoma cruzi and their evaluation for strain identification |
title_fullStr |
Further enzymic characeters of Trypanosoma cruzi and their evaluation for strain identification |
title_full_unstemmed |
Further enzymic characeters of Trypanosoma cruzi and their evaluation for strain identification |
title_sort |
Further enzymic characeters of Trypanosoma cruzi and their evaluation for strain identification |
author |
Miles, M. A |
author_facet |
Miles, M. A Lanham, Sheila M Souza, Adelson Alcimar Almeida de Póvoa, Marinete Marins |
author_role |
author |
author2 |
Lanham, Sheila M Souza, Adelson Alcimar Almeida de Póvoa, Marinete Marins |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Miles, M. A Lanham, Sheila M Souza, Adelson Alcimar Almeida de Póvoa, Marinete Marins |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Trypanosoma cruzi / classificação Trypanosoma cruzi / enzimologia Trypanosoma cruzi / isolamento & purificação |
topic |
Trypanosoma cruzi / classificação Trypanosoma cruzi / enzimologia Trypanosoma cruzi / isolamento & purificação |
description |
Starch-gel electrophoresis of 38 enzymes was attempted with extracts of Trypanosoma cruzi culture forms. 18 of the enzymes that gave discrete electrophoretic bands were selected for routine characterization of T. cruzi stocks; the enzymes were: aspartate aminotransferase (E.C.2.6.1.1, ASAT); alanine aminotransferase (E.C.2.6.1.2, ALAT); phosphoglucomutase (E.C.2.7.5.1, PGM); glucosephosphate isomerase (E.C.5.3.1.9, GPI); malate dehydrogenase (oxaloacetate decarboxylating) (NADP+) (E.C.l.l.l.40, ME); glucose 6- phosphate dehydrogenase (E.C.l.l.l.49, G6PD); malate dehydrogenase (E.C.l.l.l.37, MDH); aconitate hydratase (E.C.4.2.1.3, ACON); isocitrate dehydrogenase (NADP+) (E.C.l.l.l.42, ICD); alcohol dehydrogenase (NADP+) (E.C.l.l.l.2, ADH); lactate dehydrogenase (E.C.l.l.l.27,LDH); aminopeptidase (cytosol) (E.C.3.4.11.1, PEP); pyruvate kinase (E.C.2.7.1.40, PK); phosphoglycerate kinase (E.C.2.7.2.3, PGK); enolase (E.C.4.2.1.11, ENO); hexokinase (E.C.2.7.1.1, HK); mannosephosphate isomerase (E.C.5.3.1.8, MPI); and glutamate dehydrogenase (E.C.l.4.1.2, GD). ADH (NADP+) in the genus Trypanosoma, and PGK, MPI and ENO, in T. cruzi, were apparently demonstrated for the first time. Between six and 18 enzymes were used to characterize more than 250 T. cruzi stocks, newly isolated from a wide range of sources in northern and central Brazil. Ali stocks were identified as belonging to T. cruzi zymodemes 1, 2 or 3, as originally defined-that is, by combination of electrophoretic patterns of ASAT, ALAT, PGM, GPI, ME and G6PD. The composite range of results with ali enzymes confirmed the presence of three principal T. cruzi zymodemes, but some enzymic characters overlapped between zymodemes and others suggested subgroups within individual zymodemes. Seven (MDH, ACON, LDH, PK, PGK, ENO, HK) of the 18 enzymes did not distinguish the three zymodemes; tive (ASA T, PGM, GPI, ICD, PEP) distinguished ali three zymodemes; 10 (ASAT, ALAT, PGM, GPI, ME, G6PD, ICD, ADH, PEP, GD) distinguished zymodemes 1 and 2, of which seven plus MPI and eight plus MPI separated zymodemes 1 from 3 and 2 from 3 respectively. T. cruzi stocks were taken from a smali area of the natural species distribution; the fuli range of enzymic characters within the species T. cruzi is expected to be far more complex. The epidemiological distribution of the zymodemes continued to accord with local transmission cycles and supported the hypothesis that distinct T. cruzi strains might be responsible for the enigmatic distribution of chronic Chagas's disease. Some of the difficulties in the empirical selection of new electrophoretic methods and the interpretation of results were presented, and the present and prospective significance of T. cruzi enzymic characters was discussed. Until the stability and genetic basis of T. cruzi enzymic characters are better understood it is recommended that isoenzymic profiles be confirmed routinely, botIl before and after stocks are used experimentally, as representative of a given zymodeme. A multiple biochemical approach to T. cruzi strain identification is recommended, using characters suitable for a numerical taxonomy. |
publishDate |
1980 |
dc.date.issued.fl_str_mv |
1980 |
dc.date.accessioned.fl_str_mv |
2016-01-26T11:36:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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MILES, M. A. et al. Further enzymic characeters of Trypanosoma cruzi and their evaluation for strain identification. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 74, n. 2, p. 221-237, 1980. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/807 |
dc.identifier.issn.-.fl_str_mv |
0035-9203 |
identifier_str_mv |
MILES, M. A. et al. Further enzymic characeters of Trypanosoma cruzi and their evaluation for strain identification. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 74, n. 2, p. 221-237, 1980. 0035-9203 |
url |
https://patua.iec.gov.br/handle/iec/807 |
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eng |
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eng |
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openAccess |
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Oxford University Press |
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Oxford University Press |
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Instituto Evandro Chagas (IEC) |
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IEC |
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IEC |
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clariceneta@iec.gov.br || Biblioteca@iec.gov.br |
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