Development and evaluation of an indirect ELISA using multiepitope antigen for intestinal schistosomiasis diagnosis
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/6823 |
Resumo: | The laboratory diagnosis of intestinal schistosomiasis, carried out by detecting parasite eggs in feces, has low sensitivity when applied to individuals with low parasitic load. Serological tests can be more sensitive for the diagnosis of the disease. Therefore, the objective of this work was to develop and evaluate an ELISA-based immunoenzymatic assay, using a Schistosoma mansoni multiepitope antigen (ELISA IgG anti-SmME). For this, the amino acid sequences of S. mansoni cathepsin B and asparaginyl endopeptidase were submitted to the prediction of B cell epitopes and, together with peptide sequences obtained from earlier works, were used in the construction of a minigene. The multiepitope protein was expressed in Escherichia coli and the performance of the ELISA IgG anti-SmME for schistosomiasis was evaluated using serum samples from 107 individuals either egg positive or negative. In addition, 11 samples from individuals with other helminth infections were included. The ELISA IgG anti-SmME showed a sensitivity of 81.1% and a specificity of 46.1%. Further analysis revealed a 77.2% sensitivity in diagnosis of individuals with egg counts of ≤12 epg (eggs per gram feces) and 87.5% for individuals with 13–99 epg. It is worth mentioning that, to our knowledge, this was the first study using a multiepitope recombinant antigen in an ELISA for diagnosis of intestinal schistosomiasis, which demonstrated promising results in the diagnosis of individuals with low parasitic loads. |
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Lopes, Karine FerreiraFreire, Mariana LourençoLima, Dayane Costa SouzaEnk, Martin JohannesOliveira, EdwardGeiger, Stefan Michael2023-05-15T18:38:27Z2023-05-15T18:38:27Z2023LOPES, Karine Ferreira et al. Development and evaluation of an indirect ELISA using multiepitope antigen for intestinal schistosomiasis diagnosis. Parasitology, v. 150, n. 8, p. 683-692, 2023. DOI: https://doi.org/10.1017/S0031182023000409. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10410369/1469-8161https://patua.iec.gov.br/handle/iec/682310.1017/S0031182023000409The laboratory diagnosis of intestinal schistosomiasis, carried out by detecting parasite eggs in feces, has low sensitivity when applied to individuals with low parasitic load. Serological tests can be more sensitive for the diagnosis of the disease. Therefore, the objective of this work was to develop and evaluate an ELISA-based immunoenzymatic assay, using a Schistosoma mansoni multiepitope antigen (ELISA IgG anti-SmME). For this, the amino acid sequences of S. mansoni cathepsin B and asparaginyl endopeptidase were submitted to the prediction of B cell epitopes and, together with peptide sequences obtained from earlier works, were used in the construction of a minigene. The multiepitope protein was expressed in Escherichia coli and the performance of the ELISA IgG anti-SmME for schistosomiasis was evaluated using serum samples from 107 individuals either egg positive or negative. In addition, 11 samples from individuals with other helminth infections were included. The ELISA IgG anti-SmME showed a sensitivity of 81.1% and a specificity of 46.1%. Further analysis revealed a 77.2% sensitivity in diagnosis of individuals with egg counts of ≤12 epg (eggs per gram feces) and 87.5% for individuals with 13–99 epg. It is worth mentioning that, to our knowledge, this was the first study using a multiepitope recombinant antigen in an ELISA for diagnosis of intestinal schistosomiasis, which demonstrated promising results in the diagnosis of individuals with low parasitic loads.This study was financed by the Programa de Apoio à Pesquisa Estratégica em Saúde (PAPES VII/FIOCRUZ). E. Oliveira was supported by CNPq-Brazil (Conselho Nacional de Desenvolvimento Científico e Tecnológico; Proc. 313471/2019-3)Federal University of Minas Gerais. Institute of Biological Sciences. Department of Parasitology. Belo Horizonte, MG, Brazil / Oswaldo Cruz Foundation. René Rachou Institute. Belo Horizonte, MG, Brazil.Oswaldo Cruz Foundation. René Rachou Institute. Belo Horizonte, MG, Brazil.Federal University of Minas Gerais. Institute of Biological Sciences. Department of Parasitology. Belo Horizonte, MG, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Oswaldo Cruz Foundation. René Rachou Institute. Belo Horizonte, MG, Brazil.Federal University of Minas Gerais. Institute of Biological Sciences. Department of Parasitology. Belo Horizonte, MG, Brazil.engCambridge University PressDevelopment and evaluation of an indirect ELISA using multiepitope antigen for intestinal schistosomiasis diagnosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEsquistossomose mansoni / parasitologiaTestes LaboratoriaisCarga ParasitáriaTécnicas ImunoenzimáticasTestes de Sensibilidade Parasitária / métodosinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALDevelopment and evaluation of an indirect ELISA using a multiepitope antigen for the diagnosis of intestinal schistosomiasis.pdfapplication/pdf423285https://patua.iec.gov.br/bitstreams/5e1f31d1-c0ea-40aa-8160-c5f8481f5a62/downloadbbae54a58beffca371f9d1ad8f7684dbMD55LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/203755ec-f455-4bf1-b9ea-ae13d5ce02d4/download11832eea31b16df8613079d742d61793MD52TEXTDevelopment and evaluation of an indirect ELISA using multiepitope antigen for the diagnosis of intestinal schistosomiasis.pdf.txtDevelopment and evaluation of an indirect ELISA using multiepitope antigen for the diagnosis of intestinal schistosomiasis.pdf.txtExtracted texttext/plain62736https://patua.iec.gov.br/bitstreams/ef6bbe6e-2d27-4a96-832b-ac18bf920c4c/downloadfa977262fba4c0278b6342f30fdba48dMD56Development and evaluation of an indirect ELISA using a multiepitope antigen for the diagnosis of intestinal schistosomiasis.pdf.txtDevelopment and evaluation of an indirect ELISA using a multiepitope antigen for the diagnosis of intestinal schistosomiasis.pdf.txtExtracted texttext/plain62736https://patua.iec.gov.br/bitstreams/5019b694-ed9f-44b6-95ef-6efab55f5d34/downloadfa977262fba4c0278b6342f30fdba48dMD58THUMBNAILDevelopment and evaluation of an indirect ELISA using multiepitope antigen for the diagnosis of intestinal schistosomiasis.pdf.jpgDevelopment and evaluation of an indirect ELISA using multiepitope antigen for the diagnosis of intestinal schistosomiasis.pdf.jpgGenerated Thumbnailimage/jpeg5855https://patua.iec.gov.br/bitstreams/45021930-324c-49ad-93bd-8b78d4ed115c/download3a11936138e51212c31ee404b4dc3617MD57Development and evaluation of an indirect ELISA using a multiepitope antigen for the diagnosis of intestinal schistosomiasis.pdf.jpgDevelopment and evaluation of an indirect ELISA using a multiepitope antigen for the diagnosis of intestinal schistosomiasis.pdf.jpgGenerated Thumbnailimage/jpeg5855https://patua.iec.gov.br/bitstreams/81531b27-2d0f-4f63-9632-0e4768162931/download3a11936138e51212c31ee404b4dc3617MD59iec/68232023-08-21 18:50:01.209oai:patua.iec.gov.br:iec/6823https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-08-21T18:50:01Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv |
Development and evaluation of an indirect ELISA using multiepitope antigen for intestinal schistosomiasis diagnosis |
title |
Development and evaluation of an indirect ELISA using multiepitope antigen for intestinal schistosomiasis diagnosis |
spellingShingle |
Development and evaluation of an indirect ELISA using multiepitope antigen for intestinal schistosomiasis diagnosis Lopes, Karine Ferreira Esquistossomose mansoni / parasitologia Testes Laboratoriais Carga Parasitária Técnicas Imunoenzimáticas Testes de Sensibilidade Parasitária / métodos |
title_short |
Development and evaluation of an indirect ELISA using multiepitope antigen for intestinal schistosomiasis diagnosis |
title_full |
Development and evaluation of an indirect ELISA using multiepitope antigen for intestinal schistosomiasis diagnosis |
title_fullStr |
Development and evaluation of an indirect ELISA using multiepitope antigen for intestinal schistosomiasis diagnosis |
title_full_unstemmed |
Development and evaluation of an indirect ELISA using multiepitope antigen for intestinal schistosomiasis diagnosis |
title_sort |
Development and evaluation of an indirect ELISA using multiepitope antigen for intestinal schistosomiasis diagnosis |
author |
Lopes, Karine Ferreira |
author_facet |
Lopes, Karine Ferreira Freire, Mariana Lourenço Lima, Dayane Costa Souza Enk, Martin Johannes Oliveira, Edward Geiger, Stefan Michael |
author_role |
author |
author2 |
Freire, Mariana Lourenço Lima, Dayane Costa Souza Enk, Martin Johannes Oliveira, Edward Geiger, Stefan Michael |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Lopes, Karine Ferreira Freire, Mariana Lourenço Lima, Dayane Costa Souza Enk, Martin Johannes Oliveira, Edward Geiger, Stefan Michael |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Esquistossomose mansoni / parasitologia Testes Laboratoriais Carga Parasitária Técnicas Imunoenzimáticas Testes de Sensibilidade Parasitária / métodos |
topic |
Esquistossomose mansoni / parasitologia Testes Laboratoriais Carga Parasitária Técnicas Imunoenzimáticas Testes de Sensibilidade Parasitária / métodos |
description |
The laboratory diagnosis of intestinal schistosomiasis, carried out by detecting parasite eggs in feces, has low sensitivity when applied to individuals with low parasitic load. Serological tests can be more sensitive for the diagnosis of the disease. Therefore, the objective of this work was to develop and evaluate an ELISA-based immunoenzymatic assay, using a Schistosoma mansoni multiepitope antigen (ELISA IgG anti-SmME). For this, the amino acid sequences of S. mansoni cathepsin B and asparaginyl endopeptidase were submitted to the prediction of B cell epitopes and, together with peptide sequences obtained from earlier works, were used in the construction of a minigene. The multiepitope protein was expressed in Escherichia coli and the performance of the ELISA IgG anti-SmME for schistosomiasis was evaluated using serum samples from 107 individuals either egg positive or negative. In addition, 11 samples from individuals with other helminth infections were included. The ELISA IgG anti-SmME showed a sensitivity of 81.1% and a specificity of 46.1%. Further analysis revealed a 77.2% sensitivity in diagnosis of individuals with egg counts of ≤12 epg (eggs per gram feces) and 87.5% for individuals with 13–99 epg. It is worth mentioning that, to our knowledge, this was the first study using a multiepitope recombinant antigen in an ELISA for diagnosis of intestinal schistosomiasis, which demonstrated promising results in the diagnosis of individuals with low parasitic loads. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-05-15T18:38:27Z |
dc.date.available.fl_str_mv |
2023-05-15T18:38:27Z |
dc.date.issued.fl_str_mv |
2023 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LOPES, Karine Ferreira et al. Development and evaluation of an indirect ELISA using multiepitope antigen for intestinal schistosomiasis diagnosis. Parasitology, v. 150, n. 8, p. 683-692, 2023. DOI: https://doi.org/10.1017/S0031182023000409. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10410369/ |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/6823 |
dc.identifier.issn.-.fl_str_mv |
1469-8161 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1017/S0031182023000409 |
identifier_str_mv |
LOPES, Karine Ferreira et al. Development and evaluation of an indirect ELISA using multiepitope antigen for intestinal schistosomiasis diagnosis. Parasitology, v. 150, n. 8, p. 683-692, 2023. DOI: https://doi.org/10.1017/S0031182023000409. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10410369/ 1469-8161 10.1017/S0031182023000409 |
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https://patua.iec.gov.br/handle/iec/6823 |
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eng |
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eng |
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Cambridge University Press |
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Cambridge University Press |
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