Age and environment influences on mouse prion disease progression : behavioral changes and morphometry and stereology of hippocampal astrocytes
Main Author: | |
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Publication Date: | 2017 |
Other Authors: | , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Download full: | https://patua.iec.gov.br/handle/iec/2759 |
Summary: | Because enriched environment (EE) and exercise increase and aging decreases immune response, we hypothesized that environmental enrichment and aging will, respectively, delay and increase prion disease progression. Mice dorsal striatum received bilateral stereotaxic intracerebral injections of normal or ME7 prion infected mouse brain homogenates. After behavior analysis, animals were euthanized and their brains processed for astrocyte GFAP immunolabeling. Our analysis related to the environmental influence are limited to young adult mice, whereas age influence refers to aged mice raised on standard cages. Burrowing activity began to reduce in ME7-SE two weeks before ME7-EE, while no changes were apparent in ME7 aged mice (ME7-A). Object placement recognition was impaired in ME7-SE, NBH-A, and ME7-A but normal in all other groups. Object identity recognition was impaired in ME7-A. Cluster analysis revealed two morphological families of astrocytes in NBH-SE animals, three in NBH-A and ME7-A, and four in NBH-EE, ME7-SE, and ME7-EE. As compared with control groups, astrocytes from DG and CA3 priondiseased animals show significant numerical and morphological differences and environmental enrichment did not reverse these changes but induced different morphological changes in GFAP+ hippocampal astroglia. We suggest that environmental enrichment and aging delayed hippocampal-dependent behavioral and neuropathological signs of disease progression. |
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Torres Neto, João BentoSobral, L. LReis, R. ROliveira, R. B. deAnthony, Daniel CliveVasconcelos, Pedro Fernando da CostaDiniz, Cristovam Wanderley Picanço2017-09-21T16:57:19Z2017-09-21T16:57:19Z2017TORRES NETO, João Bento et al. Age and environment influences on mouse prion disease progression: behavioral changes and morphometry and stereology of hippocampal astrocytes. Oxidative Medicine and Cellular Longevity, v. 2017, n. 4504925, p. 1-18, 2017. DOI: https://doi.org/10.1155/2017/4504925.1942-0994https://patua.iec.gov.br/handle/iec/275910.1155/2017/4504925Because enriched environment (EE) and exercise increase and aging decreases immune response, we hypothesized that environmental enrichment and aging will, respectively, delay and increase prion disease progression. Mice dorsal striatum received bilateral stereotaxic intracerebral injections of normal or ME7 prion infected mouse brain homogenates. After behavior analysis, animals were euthanized and their brains processed for astrocyte GFAP immunolabeling. Our analysis related to the environmental influence are limited to young adult mice, whereas age influence refers to aged mice raised on standard cages. Burrowing activity began to reduce in ME7-SE two weeks before ME7-EE, while no changes were apparent in ME7 aged mice (ME7-A). Object placement recognition was impaired in ME7-SE, NBH-A, and ME7-A but normal in all other groups. Object identity recognition was impaired in ME7-A. Cluster analysis revealed two morphological families of astrocytes in NBH-SE animals, three in NBH-A and ME7-A, and four in NBH-EE, ME7-SE, and ME7-EE. As compared with control groups, astrocytes from DG and CA3 priondiseased animals show significant numerical and morphological differences and environmental enrichment did not reverse these changes but induced different morphological changes in GFAP+ hippocampal astroglia. We suggest that environmental enrichment and aging delayed hippocampal-dependent behavioral and neuropathological signs of disease progression.This study received financial support from Conselho Nacional de Pesquisa (CNPq) (Grant nos. 300203/2010-1 and 471077/2007-0) for Cristovam Wanderley Picanc¸o Diniz; Coordenação de Aperfeiçoamento de Pessoal de Níıvel Superior (CAPES) (Process no. 99999.001533/2014-02) for Cristovam Wanderley Picanço Diniz; and Universidade Federal do Para, Edital PROPESP/FADESP, PIAPA 2015.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém PA, Brazil.Universidade do Estado do Pará. Centro de Ciências da Saúde. Belém, PA, Brazil.Federal University of Rio de Janeiro. Health Sciences Center. Biomedical Sciences Institute. Vertebrate Embryology Laboratory. Rio de Janeiro, RJ, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém PA, Brazil.University of Oxford. Department of Pharmacology. Lab of Experimental Neuropathology. Oxford, UK.engHindawi Publishing CorporationAge and environment influences on mouse prion disease progression : behavioral changes and morphometry and stereology of hippocampal astrocytesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEnvelhecimento / fisiologiaDoenças Priônicas / patologiaDoenças Priônicas / psicologiaAstrócitos / patologiaCérebro / patologiaHipocampo / patologiaProgressão da DoençaComportamento AnimalMeio AmbienteModelos Animais de DoençasImuno-Histoquímica / métodosinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALAge and environment influences on mouse prion disease progression : behavioral changes and morphometry and stereology of hippocampal astrocytes.pdfAge and environment influences on mouse prion disease progression : behavioral changes and morphometry and stereology of hippocampal astrocytes.pdfapplication/pdf5704468https://patua.iec.gov.br/bitstreams/ac7ca24e-efb9-46ad-9d84-e72469478fcb/downloadfd6d72b03e874b0b86e1428dd30935e3MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/8afffdaa-baae-428f-9530-9a34efa2d523/download52f1732ea66fbd1123abe39f5373b797MD52TEXTAge and environment influences on mouse prion disease progression : behavioral changes and morphometry and stereology of hippocampal astrocytes.pdf.txtAge and environment influences on mouse prion disease progression : behavioral changes and morphometry and stereology of hippocampal astrocytes.pdf.txtExtracted texttext/plain86975https://patua.iec.gov.br/bitstreams/a90703a1-c7b6-414a-9894-ee87386ca71e/downloadf0707e298e0a68eb4047861fb9a04dd3MD55THUMBNAILAge and environment influences on mouse prion disease progression : behavioral changes and morphometry and stereology of hippocampal astrocytes.pdf.jpgAge and environment influences on mouse prion disease progression : behavioral changes and morphometry and stereology of hippocampal astrocytes.pdf.jpgGenerated Thumbnailimage/jpeg5489https://patua.iec.gov.br/bitstreams/bc54aca4-da09-42b4-9769-5dc51cf0de56/download0c06102ad5122ba6d6329102eefb9e88MD56iec/27592023-09-29 13:17:43.517oai:patua.iec.gov.br:iec/2759https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-09-29T13:17:43Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Age and environment influences on mouse prion disease progression : behavioral changes and morphometry and stereology of hippocampal astrocytes |
title |
Age and environment influences on mouse prion disease progression : behavioral changes and morphometry and stereology of hippocampal astrocytes |
spellingShingle |
Age and environment influences on mouse prion disease progression : behavioral changes and morphometry and stereology of hippocampal astrocytes Torres Neto, João Bento Envelhecimento / fisiologia Doenças Priônicas / patologia Doenças Priônicas / psicologia Astrócitos / patologia Cérebro / patologia Hipocampo / patologia Progressão da Doença Comportamento Animal Meio Ambiente Modelos Animais de Doenças Imuno-Histoquímica / métodos |
title_short |
Age and environment influences on mouse prion disease progression : behavioral changes and morphometry and stereology of hippocampal astrocytes |
title_full |
Age and environment influences on mouse prion disease progression : behavioral changes and morphometry and stereology of hippocampal astrocytes |
title_fullStr |
Age and environment influences on mouse prion disease progression : behavioral changes and morphometry and stereology of hippocampal astrocytes |
title_full_unstemmed |
Age and environment influences on mouse prion disease progression : behavioral changes and morphometry and stereology of hippocampal astrocytes |
title_sort |
Age and environment influences on mouse prion disease progression : behavioral changes and morphometry and stereology of hippocampal astrocytes |
author |
Torres Neto, João Bento |
author_facet |
Torres Neto, João Bento Sobral, L. L Reis, R. R Oliveira, R. B. de Anthony, Daniel Clive Vasconcelos, Pedro Fernando da Costa Diniz, Cristovam Wanderley Picanço |
author_role |
author |
author2 |
Sobral, L. L Reis, R. R Oliveira, R. B. de Anthony, Daniel Clive Vasconcelos, Pedro Fernando da Costa Diniz, Cristovam Wanderley Picanço |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Torres Neto, João Bento Sobral, L. L Reis, R. R Oliveira, R. B. de Anthony, Daniel Clive Vasconcelos, Pedro Fernando da Costa Diniz, Cristovam Wanderley Picanço |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Envelhecimento / fisiologia Doenças Priônicas / patologia Doenças Priônicas / psicologia Astrócitos / patologia Cérebro / patologia Hipocampo / patologia Progressão da Doença Comportamento Animal Meio Ambiente Modelos Animais de Doenças Imuno-Histoquímica / métodos |
topic |
Envelhecimento / fisiologia Doenças Priônicas / patologia Doenças Priônicas / psicologia Astrócitos / patologia Cérebro / patologia Hipocampo / patologia Progressão da Doença Comportamento Animal Meio Ambiente Modelos Animais de Doenças Imuno-Histoquímica / métodos |
description |
Because enriched environment (EE) and exercise increase and aging decreases immune response, we hypothesized that environmental enrichment and aging will, respectively, delay and increase prion disease progression. Mice dorsal striatum received bilateral stereotaxic intracerebral injections of normal or ME7 prion infected mouse brain homogenates. After behavior analysis, animals were euthanized and their brains processed for astrocyte GFAP immunolabeling. Our analysis related to the environmental influence are limited to young adult mice, whereas age influence refers to aged mice raised on standard cages. Burrowing activity began to reduce in ME7-SE two weeks before ME7-EE, while no changes were apparent in ME7 aged mice (ME7-A). Object placement recognition was impaired in ME7-SE, NBH-A, and ME7-A but normal in all other groups. Object identity recognition was impaired in ME7-A. Cluster analysis revealed two morphological families of astrocytes in NBH-SE animals, three in NBH-A and ME7-A, and four in NBH-EE, ME7-SE, and ME7-EE. As compared with control groups, astrocytes from DG and CA3 priondiseased animals show significant numerical and morphological differences and environmental enrichment did not reverse these changes but induced different morphological changes in GFAP+ hippocampal astroglia. We suggest that environmental enrichment and aging delayed hippocampal-dependent behavioral and neuropathological signs of disease progression. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-09-21T16:57:19Z |
dc.date.available.fl_str_mv |
2017-09-21T16:57:19Z |
dc.date.issued.fl_str_mv |
2017 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
TORRES NETO, João Bento et al. Age and environment influences on mouse prion disease progression: behavioral changes and morphometry and stereology of hippocampal astrocytes. Oxidative Medicine and Cellular Longevity, v. 2017, n. 4504925, p. 1-18, 2017. DOI: https://doi.org/10.1155/2017/4504925. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/2759 |
dc.identifier.issn.-.fl_str_mv |
1942-0994 |
dc.identifier.doi.-.fl_str_mv |
10.1155/2017/4504925 |
identifier_str_mv |
TORRES NETO, João Bento et al. Age and environment influences on mouse prion disease progression: behavioral changes and morphometry and stereology of hippocampal astrocytes. Oxidative Medicine and Cellular Longevity, v. 2017, n. 4504925, p. 1-18, 2017. DOI: https://doi.org/10.1155/2017/4504925. 1942-0994 10.1155/2017/4504925 |
url |
https://patua.iec.gov.br/handle/iec/2759 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
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IEC |
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IEC |
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