Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4071 |
Resumo: | In Fortaleza, the capital of Ceara State, Brazil, the detection rate of tuberculosis (TB) in 2018 was 65.5/100,000 inhabitants with a cure rate of 59.1%, which is higher than the country average. This study investigated the risk factors associated with drug-resistant tuberculosis (DR-TB) and identified the drug-resistance phenotype and resistance-conferring mutations. The geographic distribution of DR-TB in Fortaleza, Brazil, was also determined. From March 2017 to February 2018, 41 DR-TB isolates and 69 drug-susceptible pulmonary TB isolates were obtained from patients seen at a referral hospital in Fortaleza, Brazil. Samples were subjected to phenotypic and genetic analysis of resistance; the spatial distribution of the participants was also analyzed. Primary resistance was high (50.9%) among participants. The following risk factors for DR were identified: being female ( p = 0.03), having diabetes ( p < 0.01), history of previous TB disease ( p < 0.01), and the number of intra-domiciliary contacts ( p < 0.01). Analysis by multiplex allele-specific polymerase chain reaction detected mutations in the genes katG (65.8%) , rpoB (43.9%), inhA promoter (14.6%), and gyrA (9.8%). Sequencing identified mutations in the the genes katG (75.6%), inhA promoter (19.5%), rpoB (85.4%), and gyrA (100%). There was no mutation in the rrs gene. Spatial analysis showed DR-TB isolates distributed in areas of low socioeconomic status in the city of Fortaleza. Our results emphasized the importance of detecting resistance to TB drugs. The resistance found in the gene gyrA is of concern due to the high number of pre-extensive DR-TB cases in Fortaleza. |
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Campelo, Thales AlvesLima, Luana Nepomuceno Gondim CostaLima, Karla Valéria BatistaSilva, Caroliny SoaresConceição, Marília Lima daBarreto, José Antonio PereiraMota, Aquiles Paulino PeresSancho, Soraya de OliveiraFrota, Cristiane Cunha2020-03-30T17:02:34Z2020-03-30T17:02:34Z2020CAMPELO, Thales Alves et al. Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil. Revista do Instituto de Medicina Tropical, v. 62, p. 1-10, Feb. 2020.1678-9946https://patua.iec.gov.br/handle/iec/407110.1590/s1678-9946202062004In Fortaleza, the capital of Ceara State, Brazil, the detection rate of tuberculosis (TB) in 2018 was 65.5/100,000 inhabitants with a cure rate of 59.1%, which is higher than the country average. This study investigated the risk factors associated with drug-resistant tuberculosis (DR-TB) and identified the drug-resistance phenotype and resistance-conferring mutations. The geographic distribution of DR-TB in Fortaleza, Brazil, was also determined. From March 2017 to February 2018, 41 DR-TB isolates and 69 drug-susceptible pulmonary TB isolates were obtained from patients seen at a referral hospital in Fortaleza, Brazil. Samples were subjected to phenotypic and genetic analysis of resistance; the spatial distribution of the participants was also analyzed. Primary resistance was high (50.9%) among participants. The following risk factors for DR were identified: being female ( p = 0.03), having diabetes ( p < 0.01), history of previous TB disease ( p < 0.01), and the number of intra-domiciliary contacts ( p < 0.01). Analysis by multiplex allele-specific polymerase chain reaction detected mutations in the genes katG (65.8%) , rpoB (43.9%), inhA promoter (14.6%), and gyrA (9.8%). Sequencing identified mutations in the the genes katG (75.6%), inhA promoter (19.5%), rpoB (85.4%), and gyrA (100%). There was no mutation in the rrs gene. Spatial analysis showed DR-TB isolates distributed in areas of low socioeconomic status in the city of Fortaleza. Our results emphasized the importance of detecting resistance to TB drugs. The resistance found in the gene gyrA is of concern due to the high number of pre-extensive DR-TB cases in Fortaleza.This work was supported by Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico - FUNCAP (grant Nº 178638234); and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.Universidade Federal do Ceará. Faculdade de Medicina. Departamento de Patologia e Medicina Legal. Fortaleza, CE, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade do Estado do Pará. Programa de Pós-Graduação em Biologia Parasitária na Amazônia. Belém, PA, Brazil.Universidade Federal do Ceará. Faculdade de Medicina. Departamento de Patologia e Medicina Legal. Fortaleza, CE, Brazil.Universidade do Estado do Pará. Programa de Pós-Graduação em Biologia Parasitária na Amazônia. Belém, PA, Brazil.Secretaria de Saúde do Município de Fortaleza. Fortaleza, CE, Brazil.Universidade Federal do Ceará. Faculdade de Medicina. Departamento de Patologia e Medicina Legal. Fortaleza, CE, Brazil.Universidade Federal do Ceará. Faculdade de Medicina. Departamento de Patologia e Medicina Legal. Fortaleza, CE, Brazil.Universidade Federal do Ceará. Faculdade de Medicina. Departamento de Patologia e Medicina Legal. Fortaleza, CE, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade do Estado do Pará. Programa de Pós-Graduação em Biologia Parasitária na Amazônia. Belém, PA, Brazil.engUniversidade de São PauloMolecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTuberculose / diagnósticoTuberculose / tratamento farmacológicoFatores de RiscoTuberculose Resistente a Múltiplos MedicamentosResistência Microbiana a Medicamentos / imunologiaMycobacterium tuberculosis / isolamento & purificaçãoFortaleza (CE)info:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALMolecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil.pdfMolecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil.pdfapplication/pdf578436https://patua.iec.gov.br/bitstreams/d5d27439-1cb3-4a1a-ac9a-538e32fd92f4/downloadcef6da061e09a7624fb8b3949f8beb18MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil |
title |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil |
spellingShingle |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil Campelo, Thales Alves Tuberculose / diagnóstico Tuberculose / tratamento farmacológico Fatores de Risco Tuberculose Resistente a Múltiplos Medicamentos Resistência Microbiana a Medicamentos / imunologia Mycobacterium tuberculosis / isolamento & purificação Fortaleza (CE) |
title_short |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil |
title_full |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil |
title_fullStr |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil |
title_full_unstemmed |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil |
title_sort |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil |
author |
Campelo, Thales Alves |
author_facet |
Campelo, Thales Alves Lima, Luana Nepomuceno Gondim Costa Lima, Karla Valéria Batista Silva, Caroliny Soares Conceição, Marília Lima da Barreto, José Antonio Pereira Mota, Aquiles Paulino Peres Sancho, Soraya de Oliveira Frota, Cristiane Cunha |
author_role |
author |
author2 |
Lima, Luana Nepomuceno Gondim Costa Lima, Karla Valéria Batista Silva, Caroliny Soares Conceição, Marília Lima da Barreto, José Antonio Pereira Mota, Aquiles Paulino Peres Sancho, Soraya de Oliveira Frota, Cristiane Cunha |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Campelo, Thales Alves Lima, Luana Nepomuceno Gondim Costa Lima, Karla Valéria Batista Silva, Caroliny Soares Conceição, Marília Lima da Barreto, José Antonio Pereira Mota, Aquiles Paulino Peres Sancho, Soraya de Oliveira Frota, Cristiane Cunha |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Tuberculose / diagnóstico Tuberculose / tratamento farmacológico Fatores de Risco |
topic |
Tuberculose / diagnóstico Tuberculose / tratamento farmacológico Fatores de Risco Tuberculose Resistente a Múltiplos Medicamentos Resistência Microbiana a Medicamentos / imunologia Mycobacterium tuberculosis / isolamento & purificação Fortaleza (CE) |
dc.subject.decsPrimary.pt-BR.fl_str_mv |
Tuberculose Resistente a Múltiplos Medicamentos Resistência Microbiana a Medicamentos / imunologia Mycobacterium tuberculosis / isolamento & purificação Fortaleza (CE) |
description |
In Fortaleza, the capital of Ceara State, Brazil, the detection rate of tuberculosis (TB) in 2018 was 65.5/100,000 inhabitants with a cure rate of 59.1%, which is higher than the country average. This study investigated the risk factors associated with drug-resistant tuberculosis (DR-TB) and identified the drug-resistance phenotype and resistance-conferring mutations. The geographic distribution of DR-TB in Fortaleza, Brazil, was also determined. From March 2017 to February 2018, 41 DR-TB isolates and 69 drug-susceptible pulmonary TB isolates were obtained from patients seen at a referral hospital in Fortaleza, Brazil. Samples were subjected to phenotypic and genetic analysis of resistance; the spatial distribution of the participants was also analyzed. Primary resistance was high (50.9%) among participants. The following risk factors for DR were identified: being female ( p = 0.03), having diabetes ( p < 0.01), history of previous TB disease ( p < 0.01), and the number of intra-domiciliary contacts ( p < 0.01). Analysis by multiplex allele-specific polymerase chain reaction detected mutations in the genes katG (65.8%) , rpoB (43.9%), inhA promoter (14.6%), and gyrA (9.8%). Sequencing identified mutations in the the genes katG (75.6%), inhA promoter (19.5%), rpoB (85.4%), and gyrA (100%). There was no mutation in the rrs gene. Spatial analysis showed DR-TB isolates distributed in areas of low socioeconomic status in the city of Fortaleza. Our results emphasized the importance of detecting resistance to TB drugs. The resistance found in the gene gyrA is of concern due to the high number of pre-extensive DR-TB cases in Fortaleza. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-03-30T17:02:34Z |
dc.date.available.fl_str_mv |
2020-03-30T17:02:34Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CAMPELO, Thales Alves et al. Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil. Revista do Instituto de Medicina Tropical, v. 62, p. 1-10, Feb. 2020. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4071 |
dc.identifier.issn.-.fl_str_mv |
1678-9946 |
dc.identifier.doi.-.fl_str_mv |
10.1590/s1678-9946202062004 |
identifier_str_mv |
CAMPELO, Thales Alves et al. Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil. Revista do Instituto de Medicina Tropical, v. 62, p. 1-10, Feb. 2020. 1678-9946 10.1590/s1678-9946202062004 |
url |
https://patua.iec.gov.br/handle/iec/4071 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo |
dc.source.none.fl_str_mv |
reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
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Instituto Evandro Chagas (IEC) |
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IEC |
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IEC |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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