Detection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows

Detalhes bibliográficos
Autor(a) principal: Ayala, H. D. M
Data de Publicação: 2019
Outros Autores: Monteiro, E. M. M, Souza, Alex Junior Souza de, Rolim-Filho, S. T, Barbosa, E. M, Vale, W. G, Silva Filho, E, Pereira, W. L. A
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/3922
Resumo: Brucella spp. are the pathogens responsible for brucellosis, which is a cosmopolitan and serious disease for animals and humans. Recent studies have revealed the presence of Brucella DNA in the reproductive tract tissues of buffaloes based on microbiological cultures collected from vaginal swabs. We tested for Brucella abortus DNA in the uterus, perivaginal lymph nodes, and uterine mucus of buffalo cows naturally infected with Brucella spp., as defined by a positive complement fixation test. Uterine tissue, uterine mucus, and perivaginal lymph node samples from 16 Brucella seropositive female buffaloes were analyzed. Brucella DNA was found in five of the uterine tissue samples, eight of the perivaginal lymph node samples, and 14 of the uterine mucus samples. Furthermore, Bayesian inference analysis indicated that the DNA sequences obtained from these tissues and mucus grouped with corresponding sequences of the B. abortus species.
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spelling Ayala, H. D. MMonteiro, E. M. MSouza, Alex Junior Souza deRolim-Filho, S. TBarbosa, E. MVale, W. GSilva Filho, EPereira, W. L. A2019-09-20T12:50:46Z2019-09-20T12:50:46Z2019AYALA, H. D. M. et al. Detection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows. Genetics and Molecular Research, v. 18, n. 3, p. 1-7, 2019.1676-5680https://patua.iec.gov.br/handle/iec/392210.4238/gmr18376Brucella spp. are the pathogens responsible for brucellosis, which is a cosmopolitan and serious disease for animals and humans. Recent studies have revealed the presence of Brucella DNA in the reproductive tract tissues of buffaloes based on microbiological cultures collected from vaginal swabs. We tested for Brucella abortus DNA in the uterus, perivaginal lymph nodes, and uterine mucus of buffalo cows naturally infected with Brucella spp., as defined by a positive complement fixation test. Uterine tissue, uterine mucus, and perivaginal lymph node samples from 16 Brucella seropositive female buffaloes were analyzed. Brucella DNA was found in five of the uterine tissue samples, eight of the perivaginal lymph node samples, and 14 of the uterine mucus samples. Furthermore, Bayesian inference analysis indicated that the DNA sequences obtained from these tissues and mucus grouped with corresponding sequences of the B. abortus species.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal do Pará. Programa de Pós-Graduação em Ciência Animal. Belém, PA, Brasil.Universidade da Amazônia. Colegiado de Medicina Veterinária. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal Rural da Amazônia. Instituto da Saúde e Produção Animal. Belém, PA, Brasil.Universidade Federal do Amapá. Departamento de Educação do Campo. Mazagão, AP, Brasil.Universidade Federal Rural da Amazônia. Instituto da Saúde e Produção Animal. Belém, PA, Brasil.Universidade Federal Rural da Amazônia. Instituto da Saúde e Produção Animal. Belém, PA, Brasil.Universidade Federal Rural da Amazônia. Instituto da Saúde e Produção Animal. Belém, PA, Brasil.engFundação de Pesquisas Cientificas de Ribeirão PretoDetection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cowsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBovinos / microbiologiaBúfalos / microbiologiaBrucella / citologiaBrucella abortus / isolamento & purificaçãoZoonosesInfecções do Sistema Genital / veterináriainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALDetection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows.pdfDetection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows.pdfapplication/pdf207982https://patua.iec.gov.br/bitstreams/b7507113-6d9c-4a16-8d09-793a3e4b0344/download7cc81ecea19a6c367ea2e23930bca1b5MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/ba4f0b46-7bc2-4499-8e65-c7fa23e54689/download11832eea31b16df8613079d742d61793MD52TEXTDetection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows.pdf.txtDetection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows.pdf.txtExtracted texttext/plain25886https://patua.iec.gov.br/bitstreams/d14f4250-95f5-4ea1-8179-105588660f1c/download74dd6ada5b94ad43fd77ea29f1e849c5MD55THUMBNAILDetection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows.pdf.jpgDetection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows.pdf.jpgGenerated Thumbnailimage/jpeg4512https://patua.iec.gov.br/bitstreams/3f7c3131-fcad-4be9-a864-812c25511a21/download635eac6ff4dfb4bcb418bd1b712f71f7MD56iec/39222022-10-20 21:57:44.216oai:patua.iec.gov.br:iec/3922https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:57:44Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falsePGI+TGljZW7Dp2EgZGUgRGlzdHJpYnVpw6fDo28gTsOjbyBFeGNsdXNpdmE8L2I+DQoNClRvZG8gZGVwb3NpdGFudGUgZGUgbWF0ZXJpYWwgbm8gUGF0dcOhIGRldmUgY29uY2VkZXIgYW8gSW5zdGl0dXRvIEV2YW5kcm8gQ2hhZ2FzIHVtYSBMaWNlbsOnYSBkZSBEaXN0cmlidWnDp8OjbyBOw6NvIEV4Y2x1c2l2YSBwYXJhIHRvcm5hciBlIG1hbnRlciBhY2Vzc8OtdmVpcyBvcyBzZXVzIGRvY3VtZW50b3MgZW0gZm9ybWF0byBkaWdpdGFsLiANCg0KQ29tIGEgY29uY2Vzc8OjbyBkZXNzYSBsaWNlbsOnYSBuw6NvIGV4Y2x1c2l2YSwgbyBkZXBvc2l0YW50ZSBjb250aW51YSBhIHJldGVyIHRvZG9zIG9zIGRpcmVpdG9zIGRlIGF1dG9yLg0KDQpBbyBhc3NpbmFyIGUgZW50cmVnYXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKQ0KDQphKSBEZWNsYXJhIHF1ZSBjb25oZWNlIGEgcG9sw610aWNhIGRlIGNvcHlyaWdodCBkYSBlZGl0b3JhIGRlIHNldSBkb2N1bWVudG8uDQoNCmIpIENvbmNlZGUgYW8gSW5zdGl0dXRvIEV2YW5kcm8gQ2hhZ2FzIG8gZGlyZWl0byBuw6NvIGV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyIGUvb3UgZGlzdHJpYnVpciBubyA8Yj5QYXR1w6E8L2I+LCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgcG9yIHF1YWxxdWVyIG91dHJvIG1laW8uDQoNCmMpIERlY2xhcmEgcXVlIGF1dG9yaXphIG8gSW5zdGl0dXRvIEV2YW5kcm8gQ2hhZ2FzIGEgYXJxdWl2YXIgbWFpcyBkZSB1bWEgY8OzcGlhIGRlc3NlIGRvY3VtZW50byBlIGNvbnZlcnTDqi1sbywgc2VtIGFsdGVyYXIgbyBzZXUgY29udGXDumRvLCBwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgZmljaGVpcm8sIG1laW8gb3Ugc3Vwb3J0ZSwgcGFyYSBlZmVpdG9zIGRlIHNlZ3VyYW7Dp2EsIHByZXNlcnZhw6fDo28gKGJhY2t1cCkgZSBhY2Vzc28uDQoNCmQpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCBlIHF1ZSBkZXTDqW0gbyBkaXJlaXRvIGRlIGNvbmNlZGVyIGEgdGVyY2Vpcm9zIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuDQoNCmUpIERlY2xhcmEgcXVlLCBubyBjYXNvIGRvIGRvY3VtZW50byBzdWJtZXRpZG8gY29udGVyIG1hdGVyaWFsIGRvIHF1YWwgbsOjbyBkZXTDqW0gb3MgZGlyZWl0b3MgZGUgYXV0b3IsIG9idGV2ZSBhIGF1dG9yaXphw6fDo28gaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhbyBJbnN0aXR1dG8gRXZhbmRybyBDaGFnYXMgb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIHV0aWxpesOhLWxvcyBsZWdhbG1lbnRlLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGVzc2UgbWF0ZXJpYWwgY3Vqb3MgZGlyZWl0b3Mgc8OjbyBkZSB0ZXJjZWlyb3MgZXN0w6EgY2xhcmFtZW50ZSBpZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBjb250ZcO6ZG8gZG8gZG9jdW1lbnRvIGVudHJlZ3VlLg0KDQpmKSBTRSBPIERPQ1VNRU5UTyBFTlRSRUdVRSBGT1IgQkFTRUFETyBFTSBUUkFCQUxITyBGSU5BTkNJQURPIE9VIEFQT0lBRE8gUE9SIE9VVFJBIElOU1RJVFVJw4fDg08gUVVFIE7Dg08gTyBJTlNUSVRVVE8gRVZBTkRSTyBDSEFHQVMsIERFQ0xBUkEgUVVFIENVTVBSSVUgUVVBSVNRVUVSIE9CUklHQcOHw5VFUyBFWElHSURBUyBQRUxPIFJFU1BFQ1RJVk8gQ09OVFJBVE8gT1UgQUNPUkRPLg0KDQpPIEluc3RpdHV0byBFdmFuZHJvIENoYWdhcyBpZGVudGlmaWNhcsOhIGNsYXJhbWVudGUgbyhzKSBub21lKHMpIGRvKHMpIGF1dG9yKGVzKSBkb3MgZGlyZWl0b3MgZG8gZG9jdW1lbnRvIGVudHJlZ3VlIGUgbsOjbyBmYXLDoSBxdWFscXVlciBhbHRlcmHDp8OjbyBhbMOpbSBkbyBwcmV2aXN0byBuYSBhbMOtbmVhIGIpLg==
dc.title.pt_BR.fl_str_mv Detection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows
title Detection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows
spellingShingle Detection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows
Ayala, H. D. M
Bovinos / microbiologia
Búfalos / microbiologia
Brucella / citologia
Brucella abortus / isolamento & purificação
Zoonoses
Infecções do Sistema Genital / veterinária
title_short Detection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows
title_full Detection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows
title_fullStr Detection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows
title_full_unstemmed Detection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows
title_sort Detection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows
author Ayala, H. D. M
author_facet Ayala, H. D. M
Monteiro, E. M. M
Souza, Alex Junior Souza de
Rolim-Filho, S. T
Barbosa, E. M
Vale, W. G
Silva Filho, E
Pereira, W. L. A
author_role author
author2 Monteiro, E. M. M
Souza, Alex Junior Souza de
Rolim-Filho, S. T
Barbosa, E. M
Vale, W. G
Silva Filho, E
Pereira, W. L. A
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ayala, H. D. M
Monteiro, E. M. M
Souza, Alex Junior Souza de
Rolim-Filho, S. T
Barbosa, E. M
Vale, W. G
Silva Filho, E
Pereira, W. L. A
dc.subject.decsPrimary.pt_BR.fl_str_mv Bovinos / microbiologia
Búfalos / microbiologia
Brucella / citologia
Brucella abortus / isolamento & purificação
Zoonoses
Infecções do Sistema Genital / veterinária
topic Bovinos / microbiologia
Búfalos / microbiologia
Brucella / citologia
Brucella abortus / isolamento & purificação
Zoonoses
Infecções do Sistema Genital / veterinária
description Brucella spp. are the pathogens responsible for brucellosis, which is a cosmopolitan and serious disease for animals and humans. Recent studies have revealed the presence of Brucella DNA in the reproductive tract tissues of buffaloes based on microbiological cultures collected from vaginal swabs. We tested for Brucella abortus DNA in the uterus, perivaginal lymph nodes, and uterine mucus of buffalo cows naturally infected with Brucella spp., as defined by a positive complement fixation test. Uterine tissue, uterine mucus, and perivaginal lymph node samples from 16 Brucella seropositive female buffaloes were analyzed. Brucella DNA was found in five of the uterine tissue samples, eight of the perivaginal lymph node samples, and 14 of the uterine mucus samples. Furthermore, Bayesian inference analysis indicated that the DNA sequences obtained from these tissues and mucus grouped with corresponding sequences of the B. abortus species.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-09-20T12:50:46Z
dc.date.available.fl_str_mv 2019-09-20T12:50:46Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv AYALA, H. D. M. et al. Detection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows. Genetics and Molecular Research, v. 18, n. 3, p. 1-7, 2019.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/3922
dc.identifier.issn.-.fl_str_mv 1676-5680
dc.identifier.doi.-.fl_str_mv 10.4238/gmr18376
identifier_str_mv AYALA, H. D. M. et al. Detection of brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows. Genetics and Molecular Research, v. 18, n. 3, p. 1-7, 2019.
1676-5680
10.4238/gmr18376
url https://patua.iec.gov.br/handle/iec/3922
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação de Pesquisas Cientificas de Ribeirão Preto
publisher.none.fl_str_mv Fundação de Pesquisas Cientificas de Ribeirão Preto
dc.source.none.fl_str_mv reponame:Repositório Digital do Instituto Evandro Chagas (Patuá)
instname:Instituto Evandro Chagas (IEC)
instacron:IEC
instname_str Instituto Evandro Chagas (IEC)
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institution IEC
reponame_str Repositório Digital do Instituto Evandro Chagas (Patuá)
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