Astrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4509 |
Resumo: | The central nervous system is the main target of MeHg toxicity and glial cells are the first line of defense; however, their true role remains unclear. This study aimed to identify the global map of human glial-like (U87) cells transcriptome after exposure to a non-toxic and non-lethal MeHg concentration and to investigate the related molecular changes. U87 cells were exposed upon 0.1, 0.5, and 1 mu M MeHg for 4 and 24 h. Although no changes were observed in the percentage of viable cells, the metabolic viability was significantly decreased after exposure to 1 mu M MeHg for 24 h; thus, the non-toxic concentration of 0.1 mu M MeHg was chosen to perform microarray analysis. Significant changes in U87 cells transcriptome were observed only after 24 h. The expression of 392 genes was down regulated while 431 genes were up-regulated. Gene ontology showed alterations in biological processes (75%), cellular components (21%), and molecular functions (4%). The main pathways showed by KEGG and Reactome were cell cycle regulation and Rho GTPase signaling. The complex mechanism of U87 cells response against MeHg exposure indicates that even a low and non-toxic concentration is able to alter the gene expression profile. |
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Puty, BrunaBittencourt, Leonardo OliveiraPlaça Jr, Jéssica RodriguesOliveira, Edivaldo Herculano Corrêa deLima, Rafael Rodrigues2022-05-17T14:31:14Z2022-05-17T14:31:14Z2023PUTY, Bruna et al. Astrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration. Biological Trace Element Research, v. 201, n. 3, p. 1151-1162, 2023.1559-0720https://patua.iec.gov.br/handle/iec/450910.1007/s12011-022-03225-3The central nervous system is the main target of MeHg toxicity and glial cells are the first line of defense; however, their true role remains unclear. This study aimed to identify the global map of human glial-like (U87) cells transcriptome after exposure to a non-toxic and non-lethal MeHg concentration and to investigate the related molecular changes. U87 cells were exposed upon 0.1, 0.5, and 1 mu M MeHg for 4 and 24 h. Although no changes were observed in the percentage of viable cells, the metabolic viability was significantly decreased after exposure to 1 mu M MeHg for 24 h; thus, the non-toxic concentration of 0.1 mu M MeHg was chosen to perform microarray analysis. Significant changes in U87 cells transcriptome were observed only after 24 h. The expression of 392 genes was down regulated while 431 genes were up-regulated. Gene ontology showed alterations in biological processes (75%), cellular components (21%), and molecular functions (4%). The main pathways showed by KEGG and Reactome were cell cycle regulation and Rho GTPase signaling. The complex mechanism of U87 cells response against MeHg exposure indicates that even a low and non-toxic concentration is able to alter the gene expression profile.This study was financed in part by the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES)-Finance Code 001. Programa Nacional de PosGraduacao (PNPD/CAPES).Federal University of Pará. Institute of Biological Science. Laboratory of Functional and Structural Biology. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasil.Federal University of Pará. Institute of Biological Science. Laboratory of Functional and Structural Biology. Belém, PA, BrazilNational Council for Scientific and Technological Development. National Institute of Science and Technology in Stem Cell and Cell Therapy. Research Support Foundation of the State of São Paulo. Ribeirão Preto, SP, BrazilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, BrasilFederal University of Pará. Institute of Biological Science. Laboratory of Functional and Structural Biology. Belém, PA, BrazilengSpringerAstrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentrationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSistema Nervoso Central / citologiaIntoxicação do Sistema Nervoso por MercúrioCompostos de Metilmercúrio / toxicidadeNeuroglia / citologiainfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALAstrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration.pdfAstrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/8c276492-aa60-4102-9e85-64a491812ef3/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/457c9e1d-eb45-406c-9ba2-6ccc20a4e87b/download11832eea31b16df8613079d742d61793MD52TEXTAstrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration.pdf.txtAstrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/b7f6b6f3-6a92-4dd1-a971-1940176b93d7/downloade1c06d85ae7b8b032bef47e42e4c08f9MD55THUMBNAILAstrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration.pdf.jpgAstrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/85f2e741-79e2-4675-9bf7-98b07e934aea/download71859d578212107f7f8c49a4ce09d9eeMD56iec/45092023-03-08 12:29:35.251oai:patua.iec.gov.br:iec/4509https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-03-08T12:29:35Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv |
Astrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration |
title |
Astrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration |
spellingShingle |
Astrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration Puty, Bruna Sistema Nervoso Central / citologia Intoxicação do Sistema Nervoso por Mercúrio Compostos de Metilmercúrio / toxicidade Neuroglia / citologia |
title_short |
Astrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration |
title_full |
Astrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration |
title_fullStr |
Astrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration |
title_full_unstemmed |
Astrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration |
title_sort |
Astrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration |
author |
Puty, Bruna |
author_facet |
Puty, Bruna Bittencourt, Leonardo Oliveira Plaça Jr, Jéssica Rodrigues Oliveira, Edivaldo Herculano Corrêa de Lima, Rafael Rodrigues |
author_role |
author |
author2 |
Bittencourt, Leonardo Oliveira Plaça Jr, Jéssica Rodrigues Oliveira, Edivaldo Herculano Corrêa de Lima, Rafael Rodrigues |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Puty, Bruna Bittencourt, Leonardo Oliveira Plaça Jr, Jéssica Rodrigues Oliveira, Edivaldo Herculano Corrêa de Lima, Rafael Rodrigues |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Sistema Nervoso Central / citologia Intoxicação do Sistema Nervoso por Mercúrio Compostos de Metilmercúrio / toxicidade Neuroglia / citologia |
topic |
Sistema Nervoso Central / citologia Intoxicação do Sistema Nervoso por Mercúrio Compostos de Metilmercúrio / toxicidade Neuroglia / citologia |
description |
The central nervous system is the main target of MeHg toxicity and glial cells are the first line of defense; however, their true role remains unclear. This study aimed to identify the global map of human glial-like (U87) cells transcriptome after exposure to a non-toxic and non-lethal MeHg concentration and to investigate the related molecular changes. U87 cells were exposed upon 0.1, 0.5, and 1 mu M MeHg for 4 and 24 h. Although no changes were observed in the percentage of viable cells, the metabolic viability was significantly decreased after exposure to 1 mu M MeHg for 24 h; thus, the non-toxic concentration of 0.1 mu M MeHg was chosen to perform microarray analysis. Significant changes in U87 cells transcriptome were observed only after 24 h. The expression of 392 genes was down regulated while 431 genes were up-regulated. Gene ontology showed alterations in biological processes (75%), cellular components (21%), and molecular functions (4%). The main pathways showed by KEGG and Reactome were cell cycle regulation and Rho GTPase signaling. The complex mechanism of U87 cells response against MeHg exposure indicates that even a low and non-toxic concentration is able to alter the gene expression profile. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-05-17T14:31:14Z |
dc.date.available.fl_str_mv |
2022-05-17T14:31:14Z |
dc.date.issued.fl_str_mv |
2023 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PUTY, Bruna et al. Astrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration. Biological Trace Element Research, v. 201, n. 3, p. 1151-1162, 2023. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4509 |
dc.identifier.issn.-.fl_str_mv |
1559-0720 |
dc.identifier.doi.-.fl_str_mv |
10.1007/s12011-022-03225-3 |
identifier_str_mv |
PUTY, Bruna et al. Astrocyte-like cells transcriptome changes after exposure to a low and non-cytotoxic MeHg concentration. Biological Trace Element Research, v. 201, n. 3, p. 1151-1162, 2023. 1559-0720 10.1007/s12011-022-03225-3 |
url |
https://patua.iec.gov.br/handle/iec/4509 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/embargoedAccess |
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embargoedAccess |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
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reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
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Instituto Evandro Chagas (IEC) |
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IEC |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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