SELECTION OF STRAIS PRODUCING FUNGAL CHITOSANASE AND KINETICS
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Holos |
Texto Completo: | http://www2.ifrn.edu.br/ojs/index.php/HOLOS/article/view/605 |
Resumo: | In this work it was performed a selection of fungal strains able to grow in a specific medium containing chitosan (Chitosan Detector Agar) as the sole carbon source at 0.2%, and a kinetic study was performed to evaluate the best conditions for production of chitosanase from the best producing strain. The selection was made from five strains, previously isolated from soil. In the evaluation of kinetics was accompanied by cell concentration, the protein concentration (mg/mL), the enzyme activity (U/mL) for two volumes of fermentation broth (0.05 and 0.10 mL). The results showed a significant increase in all parameters studied in the first 12 hours of culture. Comparing the activity values for the two volumes of fermentation broth was observed that the volume of 0.05 mL broth showed enzymatic activity always greater than the volume of 0.1 mL |
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SELECTION OF STRAIS PRODUCING FUNGAL CHITOSANASE AND KINETICSIn this work it was performed a selection of fungal strains able to grow in a specific medium containing chitosan (Chitosan Detector Agar) as the sole carbon source at 0.2%, and a kinetic study was performed to evaluate the best conditions for production of chitosanase from the best producing strain. The selection was made from five strains, previously isolated from soil. In the evaluation of kinetics was accompanied by cell concentration, the protein concentration (mg/mL), the enzyme activity (U/mL) for two volumes of fermentation broth (0.05 and 0.10 mL). The results showed a significant increase in all parameters studied in the first 12 hours of culture. Comparing the activity values for the two volumes of fermentation broth was observed that the volume of 0.05 mL broth showed enzymatic activity always greater than the volume of 0.1 mLInstituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte2011-07-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www2.ifrn.edu.br/ojs/index.php/HOLOS/article/view/60510.15628/holos.2011.605HOLOS; v. 3 (2011); 56-631807-1600reponame:Holosinstname:Instituto Federal do Rio Grande do Norte (IFRN)instacron:IFRNporhttp://www2.ifrn.edu.br/ojs/index.php/HOLOS/article/view/605/443Copyright (c) 2016 HOLOSinfo:eu-repo/semantics/openAccessSilva Filho, Raimundo CosmeSantana, Sérgio CarvalhoKhan, Naveed KhanSantos, Everaldo SilvinoMarcedo, Gorete Ribeiro2022-05-01T20:43:52Zoai:holos.ifrn.edu.br:article/605Revistahttp://www2.ifrn.edu.br/ojs/index.php/HOLOSPUBhttp://www2.ifrn.edu.br/ojs/index.php/HOLOS/oaiholos@ifrn.edu.br||jyp.leite@ifrn.edu.br||propi@ifrn.edu.br1807-16001518-1634opendoar:2022-05-01T20:43:52Holos - Instituto Federal do Rio Grande do Norte (IFRN)false |
dc.title.none.fl_str_mv |
SELECTION OF STRAIS PRODUCING FUNGAL CHITOSANASE AND KINETICS |
title |
SELECTION OF STRAIS PRODUCING FUNGAL CHITOSANASE AND KINETICS |
spellingShingle |
SELECTION OF STRAIS PRODUCING FUNGAL CHITOSANASE AND KINETICS Silva Filho, Raimundo Cosme |
title_short |
SELECTION OF STRAIS PRODUCING FUNGAL CHITOSANASE AND KINETICS |
title_full |
SELECTION OF STRAIS PRODUCING FUNGAL CHITOSANASE AND KINETICS |
title_fullStr |
SELECTION OF STRAIS PRODUCING FUNGAL CHITOSANASE AND KINETICS |
title_full_unstemmed |
SELECTION OF STRAIS PRODUCING FUNGAL CHITOSANASE AND KINETICS |
title_sort |
SELECTION OF STRAIS PRODUCING FUNGAL CHITOSANASE AND KINETICS |
author |
Silva Filho, Raimundo Cosme |
author_facet |
Silva Filho, Raimundo Cosme Santana, Sérgio Carvalho Khan, Naveed Khan Santos, Everaldo Silvino Marcedo, Gorete Ribeiro |
author_role |
author |
author2 |
Santana, Sérgio Carvalho Khan, Naveed Khan Santos, Everaldo Silvino Marcedo, Gorete Ribeiro |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva Filho, Raimundo Cosme Santana, Sérgio Carvalho Khan, Naveed Khan Santos, Everaldo Silvino Marcedo, Gorete Ribeiro |
description |
In this work it was performed a selection of fungal strains able to grow in a specific medium containing chitosan (Chitosan Detector Agar) as the sole carbon source at 0.2%, and a kinetic study was performed to evaluate the best conditions for production of chitosanase from the best producing strain. The selection was made from five strains, previously isolated from soil. In the evaluation of kinetics was accompanied by cell concentration, the protein concentration (mg/mL), the enzyme activity (U/mL) for two volumes of fermentation broth (0.05 and 0.10 mL). The results showed a significant increase in all parameters studied in the first 12 hours of culture. Comparing the activity values for the two volumes of fermentation broth was observed that the volume of 0.05 mL broth showed enzymatic activity always greater than the volume of 0.1 mL |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-07-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www2.ifrn.edu.br/ojs/index.php/HOLOS/article/view/605 10.15628/holos.2011.605 |
url |
http://www2.ifrn.edu.br/ojs/index.php/HOLOS/article/view/605 |
identifier_str_mv |
10.15628/holos.2011.605 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www2.ifrn.edu.br/ojs/index.php/HOLOS/article/view/605/443 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2016 HOLOS info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2016 HOLOS |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte |
publisher.none.fl_str_mv |
Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte |
dc.source.none.fl_str_mv |
HOLOS; v. 3 (2011); 56-63 1807-1600 reponame:Holos instname:Instituto Federal do Rio Grande do Norte (IFRN) instacron:IFRN |
instname_str |
Instituto Federal do Rio Grande do Norte (IFRN) |
instacron_str |
IFRN |
institution |
IFRN |
reponame_str |
Holos |
collection |
Holos |
repository.name.fl_str_mv |
Holos - Instituto Federal do Rio Grande do Norte (IFRN) |
repository.mail.fl_str_mv |
holos@ifrn.edu.br||jyp.leite@ifrn.edu.br||propi@ifrn.edu.br |
_version_ |
1798951618471788544 |