Ontogenetic events in androgenesis of Brazilian barley genotypes

Detalhes bibliográficos
Autor(a) principal: SILVA,A. L. S. da
Data de Publicação: 2000
Outros Autores: MORAES-FERNANDES,M. I., FERREIRA,A. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Biologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000200016
Resumo: This paper describes a simple procedure for obtention of barley (Hordeum vulgare L.) doubled haploid plants from Brazilian hybrid genotypes. Anatomical and histological examinations showed the reversion of barley pollen to an sporophytic mode of development. A sequence of mitosis led to the formation of multicellular pollen grains. Regeneration of plants occurred either by direct embryogenesis or callus formation followed by differentiation through direct embryogenesis or organogenesis. Plants were formed in the same medium used for induction dispensing an additional regeneration step. This procedure makes doubled haploid production simpler and faster. Plantlets were transferred to another medium for rooting and after that planted in pots with vermiculite and nutrient solution.
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spelling Ontogenetic events in androgenesis of Brazilian barley genotypesanther culturedoubled haploidsThis paper describes a simple procedure for obtention of barley (Hordeum vulgare L.) doubled haploid plants from Brazilian hybrid genotypes. Anatomical and histological examinations showed the reversion of barley pollen to an sporophytic mode of development. A sequence of mitosis led to the formation of multicellular pollen grains. Regeneration of plants occurred either by direct embryogenesis or callus formation followed by differentiation through direct embryogenesis or organogenesis. Plants were formed in the same medium used for induction dispensing an additional regeneration step. This procedure makes doubled haploid production simpler and faster. Plantlets were transferred to another medium for rooting and after that planted in pots with vermiculite and nutrient solution.Instituto Internacional de Ecologia2000-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000200016Revista Brasileira de Biologia v.60 n.2 2000reponame:Revista Brasileira de Biologia (Online)instname:Instituto Internacional de Ecologia (IIE)instacron:IIE10.1590/S0034-71082000000200016info:eu-repo/semantics/openAccessSILVA,A. L. S. daMORAES-FERNANDES,M. I.FERREIRA,A. G.eng2000-08-21T00:00:00Zoai:scielo:S0034-71082000000200016Revistawww.scielo.br/rbbioONGhttps://old.scielo.br/oai/scielo-oai.php||bjb.iie@terra.com.br1806-96060034-7108opendoar:2000-08-21T00:00Revista Brasileira de Biologia (Online) - Instituto Internacional de Ecologia (IIE)false
dc.title.none.fl_str_mv Ontogenetic events in androgenesis of Brazilian barley genotypes
title Ontogenetic events in androgenesis of Brazilian barley genotypes
spellingShingle Ontogenetic events in androgenesis of Brazilian barley genotypes
SILVA,A. L. S. da
anther culture
doubled haploids
title_short Ontogenetic events in androgenesis of Brazilian barley genotypes
title_full Ontogenetic events in androgenesis of Brazilian barley genotypes
title_fullStr Ontogenetic events in androgenesis of Brazilian barley genotypes
title_full_unstemmed Ontogenetic events in androgenesis of Brazilian barley genotypes
title_sort Ontogenetic events in androgenesis of Brazilian barley genotypes
author SILVA,A. L. S. da
author_facet SILVA,A. L. S. da
MORAES-FERNANDES,M. I.
FERREIRA,A. G.
author_role author
author2 MORAES-FERNANDES,M. I.
FERREIRA,A. G.
author2_role author
author
dc.contributor.author.fl_str_mv SILVA,A. L. S. da
MORAES-FERNANDES,M. I.
FERREIRA,A. G.
dc.subject.por.fl_str_mv anther culture
doubled haploids
topic anther culture
doubled haploids
description This paper describes a simple procedure for obtention of barley (Hordeum vulgare L.) doubled haploid plants from Brazilian hybrid genotypes. Anatomical and histological examinations showed the reversion of barley pollen to an sporophytic mode of development. A sequence of mitosis led to the formation of multicellular pollen grains. Regeneration of plants occurred either by direct embryogenesis or callus formation followed by differentiation through direct embryogenesis or organogenesis. Plants were formed in the same medium used for induction dispensing an additional regeneration step. This procedure makes doubled haploid production simpler and faster. Plantlets were transferred to another medium for rooting and after that planted in pots with vermiculite and nutrient solution.
publishDate 2000
dc.date.none.fl_str_mv 2000-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000200016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000200016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0034-71082000000200016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Internacional de Ecologia
publisher.none.fl_str_mv Instituto Internacional de Ecologia
dc.source.none.fl_str_mv Revista Brasileira de Biologia v.60 n.2 2000
reponame:Revista Brasileira de Biologia (Online)
instname:Instituto Internacional de Ecologia (IIE)
instacron:IIE
instname_str Instituto Internacional de Ecologia (IIE)
instacron_str IIE
institution IIE
reponame_str Revista Brasileira de Biologia (Online)
collection Revista Brasileira de Biologia (Online)
repository.name.fl_str_mv Revista Brasileira de Biologia (Online) - Instituto Internacional de Ecologia (IIE)
repository.mail.fl_str_mv ||bjb.iie@terra.com.br
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