Conventional and molecular typing of Salmonella typhi strains from Brazil
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/30652 |
Resumo: | Phenotypic and genotypic characteristics of Salmonella Typhi were studied in 30 strains, isolated in different years, from some areas in Brazil. Conventional typing methods were performed by biochemical tests, Vi phage-typing scheme, and antimicrobial susceptibility test. Molecular typing methods were performed by analysis of plasmid DNA and by random amplified polymorphic DNA (RAPD-PCR). For the latter, an optimization step was performed to ensure the reproducibility of the process in genetic characterization of S. Typhi. The predominance of 76.7% of biotype I (xylose +, arabinose -) was noticed in all studied areas. Three phage types were recognized, with prominence for the phage types A (73.3%) and I+IV (23.3%). All the strains were susceptible to the drugs used. However, 36.7% of the strains contained plasmids, with predominance of the 105 Kb plasmid. RAPD was capable of grouping the strains in 8 genotypic patterns using primer 784, in 6, using primer 787 and in 7, using primer 797. Conventional phenotypic typing methods, as well as the DNA plasmid analysis, presented nonsignificant discriminatory power; however, RAPD-PCR analysis showed discriminatory power, reproducibility, easy interpretation and performance, being considered as a promising alternative typing method for S. Typhi. |
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Conventional and molecular typing of Salmonella typhi strains from Brazil Métodos convencionais e moleculares para tipagem de Salmonella Typhi isoladas no Brasil TypingSalmonella TyphiVi Phage-typingRandom Amplified Polymorphic DNA-Polymerase Chain ReactionBrazil Phenotypic and genotypic characteristics of Salmonella Typhi were studied in 30 strains, isolated in different years, from some areas in Brazil. Conventional typing methods were performed by biochemical tests, Vi phage-typing scheme, and antimicrobial susceptibility test. Molecular typing methods were performed by analysis of plasmid DNA and by random amplified polymorphic DNA (RAPD-PCR). For the latter, an optimization step was performed to ensure the reproducibility of the process in genetic characterization of S. Typhi. The predominance of 76.7% of biotype I (xylose +, arabinose -) was noticed in all studied areas. Three phage types were recognized, with prominence for the phage types A (73.3%) and I+IV (23.3%). All the strains were susceptible to the drugs used. However, 36.7% of the strains contained plasmids, with predominance of the 105 Kb plasmid. RAPD was capable of grouping the strains in 8 genotypic patterns using primer 784, in 6, using primer 787 and in 7, using primer 797. Conventional phenotypic typing methods, as well as the DNA plasmid analysis, presented nonsignificant discriminatory power; however, RAPD-PCR analysis showed discriminatory power, reproducibility, easy interpretation and performance, being considered as a promising alternative typing method for S. Typhi. Características fenotípicas e genotípicas de Salmonella Typhi foram estudadas em 30 amostras originárias de certas regiões do Brasil e isoladas em diferentes anos. Os métodos convencionais foram realizados através da tipagem bioquímica, da fagotipagem Vi e do teste de suscetibilidade aos antimicrobianos. Os métodos moleculares foram realizados através das análises do DNA plasmidial e do DNA polimórfico amplificado aleatoriamente (RAPD-PCR). Para este último, uma etapa de otimização foi promovida para garantir a reprodutibilidade do processo na caracterização genotípica das cepas. Foi observada a predominância de 76,7% do biotipo I (xilose +, arabinose -) em todas as regiões consideradas. Três fagotipos foram reconhecidos, com destaque para os fagotipos A (73,3%) e I+IV (23,3%). Todas as amostras demonstraram sensibilidade às drogas testadas. No entanto, 36,7 % das amostras evidenciaram plasmídios, predominando o de 105Kb. RAPD-PCR agrupou as amostras em 8 perfis genotípicos com o iniciador 784, em 6 perfis com o iniciador 787 e em 7, com o iniciador 797. Os métodos convencionais, bem como a análise do DNA plasmidial, não mostraram poder discriminatório significativo; entretanto, a análise por RAPD-PCR mostrou poder discriminatório, reprodutibilidade, fácil interpretação e execução, sendo considerada uma alternativa promissora na tipagem de S. Typhi. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2002-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30652Revista do Instituto de Medicina Tropical de São Paulo; Vol. 44 No. 6 (2002); 315-319 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 44 Núm. 6 (2002); 315-319 Revista do Instituto de Medicina Tropical de São Paulo; v. 44 n. 6 (2002); 315-319 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30652/32536Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessQUINTAES, Bianca R.LEAL, Nilma C.REIS, Eliane M. F.FONSECA, Érica L.HOFER, Ernesto2012-07-07T17:53:36Zoai:revistas.usp.br:article/30652Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:25.513864Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Conventional and molecular typing of Salmonella typhi strains from Brazil Métodos convencionais e moleculares para tipagem de Salmonella Typhi isoladas no Brasil |
title |
Conventional and molecular typing of Salmonella typhi strains from Brazil |
spellingShingle |
Conventional and molecular typing of Salmonella typhi strains from Brazil QUINTAES, Bianca R. Typing Salmonella Typhi Vi Phage-typing Random Amplified Polymorphic DNA-Polymerase Chain Reaction Brazil |
title_short |
Conventional and molecular typing of Salmonella typhi strains from Brazil |
title_full |
Conventional and molecular typing of Salmonella typhi strains from Brazil |
title_fullStr |
Conventional and molecular typing of Salmonella typhi strains from Brazil |
title_full_unstemmed |
Conventional and molecular typing of Salmonella typhi strains from Brazil |
title_sort |
Conventional and molecular typing of Salmonella typhi strains from Brazil |
author |
QUINTAES, Bianca R. |
author_facet |
QUINTAES, Bianca R. LEAL, Nilma C. REIS, Eliane M. F. FONSECA, Érica L. HOFER, Ernesto |
author_role |
author |
author2 |
LEAL, Nilma C. REIS, Eliane M. F. FONSECA, Érica L. HOFER, Ernesto |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
QUINTAES, Bianca R. LEAL, Nilma C. REIS, Eliane M. F. FONSECA, Érica L. HOFER, Ernesto |
dc.subject.por.fl_str_mv |
Typing Salmonella Typhi Vi Phage-typing Random Amplified Polymorphic DNA-Polymerase Chain Reaction Brazil |
topic |
Typing Salmonella Typhi Vi Phage-typing Random Amplified Polymorphic DNA-Polymerase Chain Reaction Brazil |
description |
Phenotypic and genotypic characteristics of Salmonella Typhi were studied in 30 strains, isolated in different years, from some areas in Brazil. Conventional typing methods were performed by biochemical tests, Vi phage-typing scheme, and antimicrobial susceptibility test. Molecular typing methods were performed by analysis of plasmid DNA and by random amplified polymorphic DNA (RAPD-PCR). For the latter, an optimization step was performed to ensure the reproducibility of the process in genetic characterization of S. Typhi. The predominance of 76.7% of biotype I (xylose +, arabinose -) was noticed in all studied areas. Three phage types were recognized, with prominence for the phage types A (73.3%) and I+IV (23.3%). All the strains were susceptible to the drugs used. However, 36.7% of the strains contained plasmids, with predominance of the 105 Kb plasmid. RAPD was capable of grouping the strains in 8 genotypic patterns using primer 784, in 6, using primer 787 and in 7, using primer 797. Conventional phenotypic typing methods, as well as the DNA plasmid analysis, presented nonsignificant discriminatory power; however, RAPD-PCR analysis showed discriminatory power, reproducibility, easy interpretation and performance, being considered as a promising alternative typing method for S. Typhi. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30652 |
url |
https://www.revistas.usp.br/rimtsp/article/view/30652 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30652/32536 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 44 No. 6 (2002); 315-319 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 44 Núm. 6 (2002); 315-319 Revista do Instituto de Medicina Tropical de São Paulo; v. 44 n. 6 (2002); 315-319 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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